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Questions and Answers
Which of the following is a limitation of using PCR to isolate genes?
Which of the following is a limitation of using PCR to isolate genes?
- PCR can only amplify small DNA fragments (correct)
- PCR cannot amplify DNA from complex samples
- PCR requires a high amount of starting DNA
- PCR is not specific to the gene of interest
What are cloning vectors used for?
What are cloning vectors used for?
- To amplify DNA fragments using PCR
- To propagate recombinant DNA in a host organism (correct)
- To analyze gene expression
- To sequence DNA fragments
What are plasmids?
What are plasmids?
- Proteins that bind to DNA
- Linear DNA molecules found in bacteria and other organisms
- Circular DNA molecules found in bacteria and other organisms (correct)
- Viruses that infect bacteria
What are the different types of plasmids?
What are the different types of plasmids?
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What are phages?
What are phages?
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What are the two main types of phage infection cycles?
What are the two main types of phage infection cycles?
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How is plasmid DNA isolation different from isolation of total bacterial DNA?
How is plasmid DNA isolation different from isolation of total bacterial DNA?
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In what conformation do plasmids exist?
In what conformation do plasmids exist?
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What enzymes are used in DNA manipulation?
What enzymes are used in DNA manipulation?
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What is gene cloning?
What is gene cloning?
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Study Notes
- Gene cloning involves inserting a DNA fragment into a vector and propagating the recombinant DNA in a host organism to produce clones.
- PCR can also be used to isolate genes, but has limitations.
- Cloning vectors, such as plasmids and bacteriophage chromosomes, are used to construct recombinant DNA molecules.
- Plasmids are circular DNA molecules found in bacteria and other organisms, and can carry genes for useful characteristics.
- Different types of plasmids include resistance, fertility, virulence, col, and degradative plasmids.
- Phages are viruses that infect bacteria and have a simple structure consisting of DNA surrounded by a capsid.
- Phages have two main types of infection cycles: lytic and lysogenic.
- Isolation of plasmid DNA involves the same general procedure as isolation of total bacterial DNA.
- Plasmids exist as supercoiled molecules and can be separated based on conformation.
- Enzymes such as nucleases, ligases, polymerases, and DNA modifying enzymes are used in DNA manipulation.
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