15 Questions
What is the role of Tris-acetate in TAE buffer?
Providing electrical conductivity
Why is EDTA included in TAE buffer?
To chelate divalent cations
Which function does EDTA perform in TAE buffer?
Chelating divalent cations
What is the purpose of adding Tris to TAE buffer?
Providing electrical conductivity
Why is it important to protect DNA from nucleases during electrophoresis?
To prevent DNA degradation
What is the purpose of adding glacial acetic acid to the solution?
To adjust the pH to 8.3
Why is it necessary to adjust the pH to 8.3 in the TAE buffer preparation?
To prevent DNA degradation by nucleases
Which component is used to dissolve Tris base in the preparation of TAE buffer?
Deionized water
What role does EDTA play in the preparation of TAE buffer?
Protect DNA from nucleases
How much EDTA solution is added in the preparation of TAE buffer?
20 ml of 0.5 M EDTA
What is the purpose of adjusting the pH to 8.3 in the preparation of TAE buffer?
To enhance the buffering capacity
Which component is used to dissolve EDTA in the preparation of TAE buffer?
Deionized water
Why is it necessary to mix the solution multiple times during TAE buffer preparation?
To ensure uniform distribution of components
What role does glacial acetic acid play in the TAE buffer preparation?
To regulate pH
Which reagent is added to enhance the stability of DNA during electrophoresis?
EDTA solution
Learn about the components of TAE electrophoresis buffer and their functions in agarose gel analysis of DNA. Explore how Tris, acetic acid, and EDTA work together to protect DNA from degradation during electrophoresis.
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