4-5: Wnt

Which modification ensures proper Wnt protein secretion?

What role do Wnt proteins play in Wnt signaling?

What is the primary function of Wnt signaling during embryonic development?

Which component directly binds to Wnt proteins to initiate the Wnt signaling pathway?

What is the ultimate effect of Wnt signaling on transcription?

Which of the following must occur for β-catenin to accumulate and enter the nucleus?

What complex does β-catenin form in the nucleus to activate Wnt target genes?

What event directly precedes β-catenin's entry into the nucleus during Wnt signaling?

Which of the following is a characteristic of Wnt proteins?

What is the result of Wnt stimulation on cytoplasmic YAP/TAZ?

Which of the following does the data suggest is a function of YAP/TAZ in Wnt OFF conditions?

Deficiency in which protein is associated with decreased β-TrCP in the destruction complex?

How does the model propose Yap and Taz function in the absence of Wnt signals?

When Axin1 is depleted, where do YAP/TAZ localize?

What key insight was revealed regarding Axin1's binding partners?

How does Wnt stimulation affect the association between YAP/TAZ and Axin1?

According to current research, which factor modulates both the Wnt and Hippo signaling pathways?

Which of the following diseases is NOT directly associated with mutations in Wnt pathway components?

Which process involving GSK3β is integral to Wnt signaling?

How does raising the dose of LRP6 experimentally affect the interaction of Axin1 with YAP/TAZ in Wnt-ON cells?

In the experiment with mice injected with tamoxifen, what was the observed outcome when the Villin-CreERT2;Apcfl/fl mice became moribund?

What does the presence of active Wnt signaling in crypt cells contribute to in the intestinal epithelium model?

How do YAP/TAZ affect tumor suppression?

What has been identified as a key step in the carcinogenesis of colorectal cancer (CRC)?

What method was used to determine that Axin1 binds to endogenous YAP and TAZ?

In which cellular compartment is YAP mostly found in confluent and untreated cells?

How does Axin1 impact Yap and Taz?

Why are YAP and TAZ defined as crucial for cell reprogramming?

How does the first finding for the Wnt pathway lead to its characterization?

What type of mammalian genomes harbor Wnt genes and how many?

What can co-receptors govern in Wnt signaling molecules?

In cell culture, what do you need when using Matrigel as it relates to the experiment?

How does glycosylation contribute to the function of Wnt proteins?

What is the significance of palmitoylation for Wnt proteins?

How does Wnt signaling influence embryonic development?

Which role does the Frizzled family of receptors play in Wnt signaling?

What is the role of Dishevelled (Dsh) in the Wnt signaling pathway?

How does glycogen synthase kinase 3 beta (GSK3β) participate in the Wnt signaling pathway?

What is the consequence of β-catenin entering the nucleus during Wnt signaling?

What describes the relationship between Wnt genes and cancer?

Which of the following is a key insight in Wnt action gained from studying various model organisms and cell lines?

Which of the following is a function of PORCN (Porcupine) enzyme in Wnt signaling?

What is essential for the transport and secretion of Wnt proteins by the Porcupine (Porc) enzyme?

What process involving Evi/Wntless endosomal vesicles is vital for Wnt secretion?

How does Wnt stimulation affect Axin1 regarding YAP/TAZ association?

Which mechanism has been identified to explain how Wnt signaling inactivation can be treated?

According to Figure3A from the content, where are YAP and TAZ expressed in the intestinal environment?

What is correct about how intestinal stem cells replenish the tissue?

How and why can Wnt signaling be beneficial?

What is correct regarding AXIN1?

What can you say regarding a cell in the absence of AXIN1?

In quiescent cells, how does YAP/TAZ effect β-catenin

How do secondary messengers drive their function in Wnt signal trasnduction?

How would the functional role of YAP and TAZ need to adapt if it were in a Wnt stimulation environment?

How can the research that combines genetic techniques determine the function of YAP and TAZ?

What is a common characteristic of tissue that has concerted activation and restriction of distinct gene-expression programs?

How could LRP6 doses play a role in Wnt-ON cells?

What is a direct cause for colorectal cancer?

When can a tissue follow to homeostasis and regeneration?

What were used to show that YAP and TAZ are integral components of the b-catenin destruction complex and Wnt signaling?

In a Wnt-OFF state, where are YAP/TAZ primarily located within the cell, and what is their function?

A cell undergoing Wnt stimulation experiences a shift in YAP/TAZ function. What is the subsequent effect of YAP/TAZ?

What function does the Retromer perform during Wnt secretion?

How does Axin1 contribute to tumor suppression?

A laboratory is investigating the effects of a novel drug on Wnt signaling in colorectal cancer cells. How could they leverage genome editing?

YAP and TAZ nuclear localization in the crypt has been determined to be which of the following?

A recent experiment was investigating the effects of Villin on mouse. What could have been a sign that can be expected to be observed?

Two sets of mice have been injected by tamoxifen, one has been initially sacrificed, what conclusion can you make?

To what extent are the triple mutant mice still alive or do they show signs of aberrant crypt hyperplasia after three weeks?

A scientist is working with Matrigel and is seeking essential conditions for crypt maintenance and growth. What should they do?

If Tamoxifen is introduced in Wnt related experiments, what can then be predicted for the mutant crypts?

What developmental process is influenced by Wnt's control over segment polarity?

How does Wnt protein palmitoylation contribute to its function?

What is the immediate effect of Wnt protein binding to Frizzled receptors?

How do co-receptors influence Wnt signaling pathways?

What is the role of Evi/Wntless in Wnt secretion?

How can Wnt signaling lead to carcinogenesis in colorectal cancer (CRC)?

What is the primary role of beta-catenin in the Wnt signaling pathway?

When Wnt is absent, what is the state of β-catenin?

What causes the functional inactivation of the destruction complex?

What is the consequence of TCF binding to Groucho in the Wnt pathway?

Based on the research paper, what is the relationship between YAP/TAZ and beta-catenin activity?

What is the proposed function of cytoplasmic YAP/TAZ in cells that are not under Wnt stimulation?

According to current understanding, how does Wnt stimulation affect cytoplasmic YAP/TAZ?

Which method was employed to detect the proteins associated with Axin1 in Wnt OFF cells?

In the absence of Axin, what is the localization pattern of YAP/TAZ?

What role do YAP and TAZ play in the context of tissue regeneration?

According to Figure 2F, how does the addition of Wnt3A impact the relationship between Axin1 and YAP/TAZ over time?

In the experiment relating to the intestine, in which specific region are both YAP and TAZ proteins expressed in dissected tissue?

After intestinal-specific knockout of APC in mice, what happens to YAP and TAZ proteins in villi, according to Figure 3A?

When control Villin-CreERT2 mice or those carrying double YAP/TAZ conditional alleles are used to explant Crypt tissue, what happens next?

What processes does YAP/TAZ control, after the Tamoxifen-mediated combined deletion?

According to Figure 4, what is affected by the loss of YAP/TAZ in the Axin1 complex?

What happens during HEK293 transfection in association to TOP-FLASH, according to Figure 4?

According to Figure 5, what can be said about YAP/TAZ and B-cantenin?

According to Figure 5, what do you need to do to perform an effective experiment in luciferase with the TOP-FLASH reporter in HEK293 cells?

According to Figure 6, what is used to propagate Stem Cells?

According to Figure 6, knocking out what is equivalent to having CH present for stem cells?

For stem cells, what can now be maintained by only doing only one direct experiment?

According to Figure 6, when discussing b-cantenin on TCF, what is also required at the same time?

After reviewing the content, which of the methods would help to show that components are integral to the b-cantenin destruction complex?

According to the content here, which of the following options can the Azzolin method show to be the double role of YAP and TAZ?