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Questions and Answers
Encapsulated shoot tips showed superior growth compared to non-encapsulated shoot tips.
True
Alginate-rich MSL encapsulation supported growth due to improved nutrient availability.
True
Non-encapsulated shoot tips produced roots and exhibited increased shoot initiation.
False
There are previous reports on simultaneous shoot and root development in stevia with multiple shoot proliferation in the same media.
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Filter paper in MSL medium was not effective for enhancing rooting response in in vitro shoots from synthetic seeds of R. serentina.
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MSL medium positively influenced efficient germination and growth of synthetic seeds in R. serpentina and T. indica.
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Efficient multiple shoot proliferation protocol with simultaneous shoot and root development from synthetic seeds of stevia cultured on MSL medium supported with filter paper has been previously reported.
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In vitro regenerated plantlets from synthetic seeds were successfully acclimatized with a 90% survival rate in cocopeat.
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Cocopeat provides optimal water retention, enhanced aeration, and ideal nutrient supply, but increases soil-borne root and fungal diseases.
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There are many reports available on acclimatization of in vitro synthetic seed-derived plantlets of stevia.
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Study Notes
Enhanced Regeneration of Stevia Plants from Synthetic Seeds
- Encapsulated shoot tips showed superior growth and development compared to non-encapsulated shoot tips
- Alginate-rich MSL encapsulation and MSL medium with filter paper supported growth due to improved nutrient availability
- Non-encapsulated shoot tips did not produce roots and exhibited reduced shoot initiation due to lack of nutrients
- No previous reports on simultaneous shoot and root development in stevia with multiple shoot proliferation in the same media
- Filter paper (M-bridge) in MSL medium was effective for enhancing rooting response in in vitro shoots from synthetic seeds of R. serentina
- MSL medium positively influenced efficient germination and growth of synthetic seeds in R. serpentina and T. indica
- First report of an efficient multiple shoot proliferation protocol with simultaneous shoot and root development from synthetic seeds of stevia cultured on MSL medium supported with filter paper
- In vitro regenerated plantlets from synthetic seeds were successfully acclimatized with a 90% survival rate in cocopeat
- Cocopeat provides optimal water retention, enhanced aeration, and ideal nutrient supply, minimizing soil-borne root and fungal diseases
- Few reports available on acclimatization of in vitro synthetic seed-derived plantlets of stevia
- Genetic integrity of in vitro regenerants produced from encapsulated shoot tips and mother plant was confirmed using 10 ISSR primers
- 100% genetic integrity without deviation in the banding pattern was observed in the regenerants, affirming the clonal fidelity of the regenerants
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Description
Test your knowledge about the enhanced regeneration of stevia plants from synthetic seeds with this quiz. Explore topics such as encapsulation, shoot and root development, media influence, acclimatization, and genetic integrity of in vitro regenerants.
