Sterilization Techniques Overview
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Questions and Answers

How long should the petri dish be allowed to solidify?

  • 15 minutes
  • 25 minutes
  • 20 minutes
  • 30 minutes (correct)

What is the purpose of plating cells on the petri dish?

  • To weigh the cells
  • To dilute the cells
  • To sterilize the cells
  • To culture and grow the cells (correct)

In biosafety level 2, what is a characteristic of the pathogens handled?

  • Pathogens that pose minimal risk to human health (correct)
  • Untreatable pathogens with high mortality rates
  • Pathogens that can infect through air transmission
  • Highly toxic with no treatments available

How is the accuracy of pipetting determined in Project 1?

<p>By calculating the standard deviation and coefficient of variation (D)</p> Signup and view all the answers

What is the spectrophotometer used for in the experiments described?

<p>To read the absorbance of the samples (A)</p> Signup and view all the answers

What is the purpose of using Grubb's test in the experiments?

<p>To identify any outlier data points (B)</p> Signup and view all the answers

What is the main advantage of autoclave sterilization over oven drying?

<p>More effective in killing all organisms (A)</p> Signup and view all the answers

What is the purpose of filter sterilization with a pore size of 0.22 or less?

<p>To retain amino acids and antibodies (D)</p> Signup and view all the answers

What is the correct protocol for disposing of Agar plates containing microbial cultures?

<p>Discard in an orange plastic biohazard bag (C)</p> Signup and view all the answers

Why are plates incubated upside down during the streaking technique?

<p>To prevent condensation from dripping onto colonies (B)</p> Signup and view all the answers

When estimating the cell number using optical density, what does an OD600 of 1 indicate?

<p>5E8 cells/mL (A)</p> Signup and view all the answers

What is the purpose of performing serial dilution for estimating viable cells?

<p>To obtain a countable range of colonies (B)</p> Signup and view all the answers

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