12 Questions
How long should the petri dish be allowed to solidify?
30 minutes
What is the purpose of plating cells on the petri dish?
To culture and grow the cells
In biosafety level 2, what is a characteristic of the pathogens handled?
Pathogens that pose minimal risk to human health
How is the accuracy of pipetting determined in Project 1?
By calculating the standard deviation and coefficient of variation
What is the spectrophotometer used for in the experiments described?
To read the absorbance of the samples
What is the purpose of using Grubb's test in the experiments?
To identify any outlier data points
What is the main advantage of autoclave sterilization over oven drying?
More effective in killing all organisms
What is the purpose of filter sterilization with a pore size of 0.22 or less?
To retain amino acids and antibodies
What is the correct protocol for disposing of Agar plates containing microbial cultures?
Discard in an orange plastic biohazard bag
Why are plates incubated upside down during the streaking technique?
To prevent condensation from dripping onto colonies
When estimating the cell number using optical density, what does an OD600 of 1 indicate?
5E8 cells/mL
What is the purpose of performing serial dilution for estimating viable cells?
To obtain a countable range of colonies
Learn about different sterilization techniques that eliminate all living organisms, including autoclave, filter sterilization, and gamma ray sterilization. Understand how each method works and its applications in various industries.
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