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Sterilization Techniques Overview

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Questions and Answers

How long should the petri dish be allowed to solidify?

15 minutes

25 minutes

20 minutes

30 minutes (correct)

What is the purpose of plating cells on the petri dish?

To weigh the cells

To dilute the cells

To sterilize the cells

To culture and grow the cells (correct)

In biosafety level 2, what is a characteristic of the pathogens handled?

Pathogens that pose minimal risk to human health (correct)

Untreatable pathogens with high mortality rates

Pathogens that can infect through air transmission

Highly toxic with no treatments available

How is the accuracy of pipetting determined in Project 1?

By calculating the standard deviation and coefficient of variation Signup and view all the answers

What is the spectrophotometer used for in the experiments described?

To read the absorbance of the samples Signup and view all the answers

What is the purpose of using Grubb's test in the experiments?

To identify any outlier data points Signup and view all the answers

What is the main advantage of autoclave sterilization over oven drying?

More effective in killing all organisms Signup and view all the answers

What is the purpose of filter sterilization with a pore size of 0.22 or less?

To retain amino acids and antibodies Signup and view all the answers

What is the correct protocol for disposing of Agar plates containing microbial cultures?

Discard in an orange plastic biohazard bag Signup and view all the answers

Why are plates incubated upside down during the streaking technique?

To prevent condensation from dripping onto colonies Signup and view all the answers

When estimating the cell number using optical density, what does an OD600 of 1 indicate?

5E8 cells/mL Signup and view all the answers

What is the purpose of performing serial dilution for estimating viable cells?

To obtain a countable range of colonies Signup and view all the answers