Sterilization and Disinfection Practicals

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Questions and Answers

What is the standard setting temperature for sterilizing materials in a hot air oven?

  • 200 - 220°C
  • 120 - 150°C
  • 160 - 180°C (correct)
  • 140 - 160°C

What is the primary method used for sterilizing materials and media used for culturing microorganisms?

  • Chemical sterilization
  • Heat sterilization
  • Autoclaving (correct)
  • Radiation sterilization

What is the temperature to which materials are heated during autoclaving for efficient sterilization?

  • 121°C (correct)
  • 150°C
  • 200°C
  • 100°C

What is the purpose of using an autoclave in microbiology laboratories?

<p>To sterilize materials and media used for culturing microorganisms (B)</p> Signup and view all the answers

What is the pressure range typically used during autoclaving for efficient sterilization?

<p>~15 PSI (1.5 atm) (A)</p> Signup and view all the answers

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Study Notes

Disinfection and Sterilization

  • Disinfection is a procedure that reduces microorganisms as much as possible, but not including bacterial spores, using physical and chemical means.
  • Sterilization is the complete removal or destruction of all forms of microbial life, including bacterial spores.

Sterilization Methods

  • Sterilization can be achieved by physical, chemical, and mechanical means.
  • Different types of organisms have varying resistance to killing agents.

Physical Methods of Sterilization

Heat Sterilization

  • Dry heat: • Direct flaming: loops, wires, glass slides, and instrument tips are held in a Bunsen flame until red-hot. • Hot air oven:
  • Moist heat: • Radiation Sterilization:
    • Ionized radiation:
    • Non-ionized radiation:

Chemical Methods of Sterilization

  • Alcoholic flaming: for sterilizing forceps, scissors, scalpels, triangular spreaders (metals), etc.
  • Materials can be dipped in a disinfectant before flaming.

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