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Questions and Answers
What is the standard setting temperature for sterilizing materials in a hot air oven?
What is the standard setting temperature for sterilizing materials in a hot air oven?
- 200 - 220°C
- 120 - 150°C
- 160 - 180°C (correct)
- 140 - 160°C
What is the primary method used for sterilizing materials and media used for culturing microorganisms?
What is the primary method used for sterilizing materials and media used for culturing microorganisms?
- Chemical sterilization
- Heat sterilization
- Autoclaving (correct)
- Radiation sterilization
What is the temperature to which materials are heated during autoclaving for efficient sterilization?
What is the temperature to which materials are heated during autoclaving for efficient sterilization?
- 121°C (correct)
- 150°C
- 200°C
- 100°C
What is the purpose of using an autoclave in microbiology laboratories?
What is the purpose of using an autoclave in microbiology laboratories?
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What is the pressure range typically used during autoclaving for efficient sterilization?
What is the pressure range typically used during autoclaving for efficient sterilization?
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Study Notes
Disinfection and Sterilization
- Disinfection is a procedure that reduces microorganisms as much as possible, but not including bacterial spores, using physical and chemical means.
- Sterilization is the complete removal or destruction of all forms of microbial life, including bacterial spores.
Sterilization Methods
- Sterilization can be achieved by physical, chemical, and mechanical means.
- Different types of organisms have varying resistance to killing agents.
Physical Methods of Sterilization
Heat Sterilization
- Dry heat: • Direct flaming: loops, wires, glass slides, and instrument tips are held in a Bunsen flame until red-hot. • Hot air oven:
- Moist heat:
• Radiation Sterilization:
- Ionized radiation:
- Non-ionized radiation:
Chemical Methods of Sterilization
- Alcoholic flaming: for sterilizing forceps, scissors, scalpels, triangular spreaders (metals), etc.
- Materials can be dipped in a disinfectant before flaming.
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