Sterilization and Disinfection Practicals

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5 Questions

What is the standard setting temperature for sterilizing materials in a hot air oven?

160 - 180°C

What is the primary method used for sterilizing materials and media used for culturing microorganisms?


What is the temperature to which materials are heated during autoclaving for efficient sterilization?


What is the purpose of using an autoclave in microbiology laboratories?

To sterilize materials and media used for culturing microorganisms

What is the pressure range typically used during autoclaving for efficient sterilization?

~15 PSI (1.5 atm)

Study Notes

Disinfection and Sterilization

  • Disinfection is a procedure that reduces microorganisms as much as possible, but not including bacterial spores, using physical and chemical means.
  • Sterilization is the complete removal or destruction of all forms of microbial life, including bacterial spores.

Sterilization Methods

  • Sterilization can be achieved by physical, chemical, and mechanical means.
  • Different types of organisms have varying resistance to killing agents.

Physical Methods of Sterilization

Heat Sterilization

  • Dry heat: • Direct flaming: loops, wires, glass slides, and instrument tips are held in a Bunsen flame until red-hot. • Hot air oven:
  • Moist heat: • Radiation Sterilization:
    • Ionized radiation:
    • Non-ionized radiation:

Chemical Methods of Sterilization

  • Alcoholic flaming: for sterilizing forceps, scissors, scalpels, triangular spreaders (metals), etc.
  • Materials can be dipped in a disinfectant before flaming.

Test your knowledge on sterilization and disinfection procedures, including the difference between the two processes and their effectiveness in eliminating microorganisms. Explore the significance of sterilization and disinfection in maintaining a clean and safe environment.

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