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Questions and Answers
Questions and Answers
What is the standard setting temperature for a hot air oven used to sterilize petri dishes and test tubes?
What is the standard setting temperature for a hot air oven used to sterilize petri dishes and test tubes?
- 140 - 160°C
- 160 - 180°C (correct)
- 120 - 150°C
- 200 - 220°C
What is the most common device used for sterilization via autoclaving?
What is the most common device used for sterilization via autoclaving?
- Microscope
- Autoclave (correct)
- Incubator
- Centrifuge
What temperature is used in autoclaves to sterilize materials?
What temperature is used in autoclaves to sterilize materials?
- 100°C
- 200°C
- 121°C (correct)
- 150°C
How long must materials be heated in an autoclave for efficient sterilization?
How long must materials be heated in an autoclave for efficient sterilization?
What type of radiation is used for sterilization?
What type of radiation is used for sterilization?
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Flashcards
Flashcards
Hot air oven sterilization temperature
Hot air oven sterilization temperature
160-180°C
Autoclave device
Autoclave device
A device used for sterilization by steam under pressure
Autoclave sterilization temperature
Autoclave sterilization temperature
121°C
Autoclave sterilization time
Autoclave sterilization time
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Radiation sterilization type
Radiation sterilization type
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Flashcards
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Study Notes
Study Notes
Disinfection and Sterilization Overview
- Disinfection: Procedure aimed at reducing microorganisms significantly but does not eliminate bacterial spores; employs physical and chemical methods.
- Sterilization: Complete removal or destruction of all forms of microbial life, including bacterial spores, with no absolute guarantee of zero microorganisms remaining.
Methods of Sterilization
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Physical Methods:
- Heat: Effective in killing microorganisms; includes dry heat and moist heat.
- Radiation: Uses ionized and non-ionized radiation to achieve sterilization.
-
Chemical Methods: Involves the use of various chemical agents to destroy microorganisms.
Physical Methods of Sterilization
- Heat Sterilization:
- Dry Heat: Utilizes high temperatures to kill microorganisms.
- Direct Flaming: Involves holding metal instruments (loops, wires, glass slides) in a Bunsen flame until red-hot; can be pre-treated with disinfectants.
- Alcoholic Flaming: Sterilizes metal tools (forceps, scissors, scalpels) effectively using flames from alcohol-soaked materials.
- Moist Heat: Involves steam under pressure (e.g., autoclaving) to achieve higher efficiency in killing microorganisms.
- Dry Heat: Utilizes high temperatures to kill microorganisms.
Importance of Understanding Resistance
- Different microorganisms exhibit varying levels of resistance to sterilization methods, making it crucial to select appropriate techniques for effective sterilization.
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