Sect 9 Quiz Easy

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Questions and Answers

What is the term used to describe the development history of a cell from its birth to its final differentiation?

  • Cell lineage
  • Cell niche
  • Cell asymmetry
  • Cell fate (correct)

Which statement best describes stem cells?

  • Unspecialized cells that can self-renew and differentiate (correct)
  • Cells that only multiply without differentiation
  • Specialized cells that cannot reproduce
  • Differentiated cells from adult tissues

What characterizes adult stem cells?

  • Developed from a stem cell in adult tissues (correct)
  • Found only in embryos
  • Totipotent in nature
  • Exclusively pluripotent

How can cell fate in an embryo be determined?

<p>By cell location experiments (B)</p> Signup and view all the answers

Which of the following is NOT a category of stem cell?

<p>Invasive stem cells (B)</p> Signup and view all the answers

What is the primary role of the apoptosome?

<p>To initiate cell death (D)</p> Signup and view all the answers

What defines asymmetric cell division?

<p>Produces daughter cells with different developmental fates (B)</p> Signup and view all the answers

Which term describes stem cells that can give rise to all cell types of the organism?

<p>Totipotent (A)</p> Signup and view all the answers

What type of cells can multipotent cells develop into?

<p>More than one but not all cell types (B)</p> Signup and view all the answers

What do transiently amplifying cells do?

<p>Divide rapidly and produce lineage-restricted progenitor cells (D)</p> Signup and view all the answers

How frequently does the intestinal epithelium renew itself in adult humans?

<p>Every 5 days (A)</p> Signup and view all the answers

What is the role of the niche in stem cell maintenance?

<p>Maintain a self-renewing population of undifferentiated stem cells (B)</p> Signup and view all the answers

Where do intestinal stem cells reside?

<p>At the base of the crypts in the intestinal wall (C)</p> Signup and view all the answers

What does the Wnt signaling pathway influence in intestinal stem cells?

<p>Differentiation and self-renewal (A)</p> Signup and view all the answers

What do Lgr5+ stem cells differentiate into?

<p>Various differentiated epithelial cells in the villus (B)</p> Signup and view all the answers

Which stem cells replenish needed blood cells?

<p>Hematopoietic stem cells (A)</p> Signup and view all the answers

What type of stem cells are obtained from fetuses?

<p>Fetal stem cells (D)</p> Signup and view all the answers

What is the differentiation potential of embryonic stem cells?

<p>Pluripotent (D)</p> Signup and view all the answers

At which stage of development are totipotent cells found?

<p>8-cell stage (B)</p> Signup and view all the answers

What is the primary characteristic of induced pluripotent stem cells (iPS)?

<p>Reprogrammed adult cells (C)</p> Signup and view all the answers

What are the cells found in the inner cell mass of the blastocyst called?

<p>Embryonic stem cells (D)</p> Signup and view all the answers

Which of the following statements is true about adult stem cells?

<p>They are multipotent, except germ-line stem cells. (A)</p> Signup and view all the answers

Which factors control the pluripotency of embryonic stem cells?

<p>DNA methylation and transcription factors (C)</p> Signup and view all the answers

What unique capability do embryonic stem cells have that adult stem cells do not?

<p>They can reproduce indefinitely in culture. (C)</p> Signup and view all the answers

What do Lgr5+ cells differentiate into when Notch signaling is present?

<p>Absorptive enterocytes (B)</p> Signup and view all the answers

What is the primary function of Paneth cells?

<p>Providing antimicrobial defense (A)</p> Signup and view all the answers

Which cells can restore Lgr5+ stem cells following injury?

<p>Reserve stem cells (C)</p> Signup and view all the answers

What do telocytes primarily provide in the intestinal environment?

<p>Growth factors necessary for stem-cell self-renewal (B)</p> Signup and view all the answers

Which type of cells are specialized in secreting mucus?

<p>Goblet cells (A)</p> Signup and view all the answers

What is the role of iPS cells in medical research?

<p>To understand disease causes and screen drugs (B)</p> Signup and view all the answers

Which type of cells take up intestinal microbial antigens?

<p>M cells (C)</p> Signup and view all the answers

What do symmetric cell divisions produce?

<p>Two identical daughter cells (A)</p> Signup and view all the answers

What is required for asymmetric cell division to occur?

<p>An intrinsic program to generate polarity (C)</p> Signup and view all the answers

What happens to daughter cells if the mitotic spindle is not appropriately oriented?

<p>They may have the same fate determinants (D)</p> Signup and view all the answers

What is one of the steps in generating polarized cells?

<p>Initiating a signal transduction pathway (C)</p> Signup and view all the answers

What are cell polarity determinants required for?

<p>To ensure asymmetric localization for proper cell function (D)</p> Signup and view all the answers

What triggers apoptosis in vertebrate cells?

<p>Activation of caspases (C)</p> Signup and view all the answers

Which of the following is a component of conserved apoptotic pathways?

<p>Cell membrane receptors (C)</p> Signup and view all the answers

What is the primary role of trophic factors in vertebrate cells?

<p>To ensure cell survival (B)</p> Signup and view all the answers

How is cell polarity reinforced after initial establishment?

<p>Via reinforcement of cell polarity determinants (B)</p> Signup and view all the answers

What is the primary role of caspases in cell death?

<p>To cleave specific intracellular substrates (D)</p> Signup and view all the answers

What is the outcome of apoptotic cell fragmentation?

<p>Release of small membrane-bound apoptotic bodies (D)</p> Signup and view all the answers

Which protein family is involved in the regulation of apoptosis?

<p>Bcl-2 family (B)</p> Signup and view all the answers

What triggers the oligomerization of Bax or Bak proteins?

<p>Environmental stress (B)</p> Signup and view all the answers

How do pro-apoptotic BH3-only proteins promote apoptosis?

<p>By binding directly to Bcl-2 (C)</p> Signup and view all the answers

What effect does the binding of extracellular trophic factors have on apoptosis?

<p>Triggers changes leading to cell survival (D)</p> Signup and view all the answers

What happens to Bad protein upon phosphorylation?

<p>It becomes sequestered by 14-3-3 proteins (D)</p> Signup and view all the answers

Which event triggers the synthesis of the Puma gene?

<p>DNA damage (A)</p> Signup and view all the answers

Flashcards

Stem Cell

An unspecialized cell capable of self-renewal and differentiating into specialized cells.

Cell Fate

The developmental path a cell takes, from its birth to differentiation into a specific type.

Cell Lineage

The chronological order of a cell's descent, showing how it relates to other cells in development.

Apoptosis

Programmed cell death.

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Apoptosome

A protein complex involved in initiating apoptosis.

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Asymmetric Cell Division

Cell division that produces two dissimilar daughter cells.

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Symmetric Cell Division

Cell division that produces two identical daughter cells.

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Bcl-2 Family

A family of proteins that regulate apoptosis.

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Caspases

A family of enzymes that carry out apoptosis.

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Multipotent

Stem cells that can differentiate into a limited number of cell types.

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Pluripotent

Stem cells that can differentiate into many cell types.

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Totipotent

Stem cells that can differentiate into any cell type, including extraembryonic tissues.

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Progenitor cells

Cells that are committed to a specific lineage and are capable of differentiating into specific cell types, but don't have ongoing self-renewal ability.

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Embryonic stem cells

Stem cells derived from the inner cell mass of a blastocyst.

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iPS cells

Induced pluripotent stem cells.

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Fetal Stem Cells

Stem cells taken from fetuses, more powerful and versatile than adult stem cells.

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Embryonic Stem Cells

Stem cells from a blastocyst (early embryo), highly potent.

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Induced Pluripotent Stem Cells (iPS)

Adult cells reprogrammed to act like embryonic stem cells.

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Totipotent Cells

Cells that can form all tissues of an organism, including embryonic and extraembryonic tissues.

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Pluripotent Cells

Cells that can form all tissues of the embryo but not extraembryonic tissues.

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Multipotent Cells

Stem cells capable of differentiating into multiple, closely related cell types.

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Unipotent Cells

Stem cells that can only differentiate into one specific cell type.

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8-cell stage

Embryonic stage where each cell has the potential to form all tissue types.

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Blastocyst stage

Embryo stage distinguished by inner cell mass and capable of producing embryonic stem cells.

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Adult Stem Cells

Stem cells in adults, responsible for replacement of cells, such as stomach lining and immune cells, that have limited lifespans.

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Asymmetric Cell Division

A type of cell division where daughter cells inherit different components, like mRNA and proteins, despite receiving identical genes.

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Cell Polarity

The process by which a cell develops an internal directionality, allowing for unequal distribution of cell components during division.

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Cell Polarity Determinants

Molecules (mRNA, proteins, lipids) that help establish and maintain cell polarity by being unequally distributed.

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Mitotic Spindle Orientation

The alignment of the mitotic spindle dictates how cell polarity determinants are segregated into daughter cells.

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Trophic Factors

Survival signals that vertebrate cells need to stay alive.

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Apoptosis

Programmed cell death, a controlled process of cell suicide essential for development and maintenance.

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Apoptotic Pathway Components

Membrane-bound, cytosolic regulatory proteins, and caspases (proteases) work together to carry out apoptosis.

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Multipotent cells

Stem cells that can differentiate into multiple cell types but are limited compared to pluripotent cells.

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Pluripotent cells

Stem cells that can differentiate into almost any cell type in the body.

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Stem cell population

The number of stem cells of a specific type remains constant or increases during an organism's life.

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Transiently amplifying cells

Daughter cells of stem cells that rapidly divide, eventually forming lineage-restricted progenitor cells.

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Hematopoietic stem cells

Stem cells that replenish all types of blood cells.

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Progenitor and precursor cells

Cells that proliferate (multiply) in response to cytokines (chemical messengers).

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Intestinal stem cells

Stem cells constantly making new intestinal cells.

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Absorptive enterocytes

Specialized intestinal cells that absorb nutrients.

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Intestinal epithelium turnover

The replacement of intestinal cells every few days (e.g., 5 days in humans).

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Intestinal stem cell niche

The environment that supports and regulates intestinal stem cells, keeping them undifferentiated.

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Lgr5 receptor

A receptor found on intestinal stem cells.

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Wnt pathway

A signaling pathway involved in maintaining stem cells without proliferation or differentiation, especially in the intestinal niche.

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Lgr5+ cells

Stem cells in the intestine that differentiate into either absorptive or secretory cells, depending on Notch signaling.

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Paneth cells

Intestinal cells that produce antimicrobial proteins to protect against bacteria.

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Notch signaling

A signaling pathway that influences cell fate, particularly in the intestine, determining if Lgr5+ cells become absorptive or secretory cells.

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Transit amplifying cells

Cells that undergo rapid cell division and contribute to the renewal of intestinal epithelial cells.

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Stem cells (reserve)

Specialized cells capable of self-renewal and differentiation, providing a back-up pool for replacement of injured cells. Located in the crypt.

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Telocytes

Cells with long processes that form a network in the intestine and provide growth factors for stem cells.

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Enteroendocrine cells

Specialized cells in the intestine that produce and release hormones in response to various stimuli.

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Tuft cells

Cells that act as chemosensors, detecting chemicals in the intestines and respiratory tract.

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Goblet cells

Cells that secrete mucus to protect and lubricate the intestinal lining.

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M cells

Specialized immune cells that absorb and process antigens from the gut.

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iPS cells

Induced pluripotent stem cells generated from adult cells by introducing specific transcription factors.

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Symmetric cell division

A cell division process where two identical daughter cells are produced.

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Caspase activation

Caspases are enzymes that initiate cell death. Their activation is triggered by specific cellular signals.

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Apoptotic cell

A cell undergoing programmed cell death (apoptosis).

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Bcl-2 family proteins

A family of proteins that regulate apoptosis by controlling the activity of Bax/Bak or promoting cell life. They affect mitochondrial function.

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Cytochrome c release

The release of cytochrome c from mitochondria into the cytosol is a critical step in triggering apoptosis.

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Apoptosis-inducing signal

A signal that triggers apoptosis, often involving interactions in the Bcl-2 family.

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Apoptotic Body

Small membrane-bound fragments released from an apoptotic cell.

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Necrotic cell

A cell that dies due to cellular damage or injury.

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Trophic Factors

Extracellular signaling molecules that maintain cell survival and promote cell life

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DNA damage

Causes the activation of pro-apoptotic proteins, often triggered due to cellular stress

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Study Notes

Stem Cells, Cell Asymmetry, and Cell Death

  • Learning Objectives: After completing this module, students will be able to define apoptosis, apoptosome, asymmetric/symmetric cell division, Bcl-2 family, caspases, cell fate, cell lineage, embryonic stem cells, iPS cells, multipotent, pluripotent, progenitor cells, stem cells, stem-cell niche survival signals, and totipotent.

  • Differentiate different stem cell categories used in biological research & therapy.

  • Discuss key stem cells in the intestinal stem cell niche.

  • Compare apoptosis to necrosis.

  • Text: Lodish et al. (2021), Chapter 22. Discusses the origin of people from Noah's sons. Encourages kindness and love.

  • Cell Lineage: Developmental history of a cell from birth to final division/differentiation into a specific cell type is called cell fate. A cell lineage tracks birth order of cells as they progressively specialize. Examples include cell lineage in C. elegans and all cells in that organism.

  • Stem Cells: Unspecialized cells that self-renew and differentiate into specialized cells are called stem cells. There are four main types:

    • Adult stem cells: Derived from adult tissue, possessing less differentiation potential compared to fetal cells.
    • Fetal stem cells: Derived from fetuses, capable of greater differentiation (compared to adult stem cells).
    • Embryonic stem cells (ESCs): Derived from blastocysts (4-5 days after fertilization) having the most differentiation potential among stem cells.
    • Induced pluripotent stem cells (iPSCs): Adult cells reprogrammed to have stem cell capabilities.
  • Totipotent cells: Human embryos (8-cell stage) are fully capable of forming all tissues (embryonic and extra-embryonic).

  • ES cells: At the blastocyst stage, a distinct inner cell mass forms. These become embryonic stem cells (ES cells) that can generate all embryonic tissues but not extraembryonic tissues (like placenta). ES cells are pluripotent.

  • Pluripotent cells: Can generate all cell types in the body.

  • Multipotent cells: Can develop into multiple cell types, but their differentiation capacity is more limited compared to pluripotent cells.

Cell Polarity and Asymmetric Cell Division

  • Symmetric cell divisions: Daughter cells are identical to the parent.
  • Asymmetric cell divisions: Daughter cells inherit different components, leading to various shapes or functionalities.

Cell Death

  • Apoptosis: Programmed cell death. Vertebrate cells require trophic factors for survival. Without these, apoptosis occurs. Apoptosis involves membrane-bound and cytosol regulatory proteins, and specific caspases. Activated caspases cleave intracellular substrates, leading to cell demise. Other proteins (CED-4/Apaf-1) are also involved in this process. Apoptotic cells shrink, condense, and fragment, releasing membrane-bound bodies, which are then engulfed by neighboring phagocytes. This differs from necrosis, where cells swell and burst, releasing intracellular contents causing inflammation.

Bcl-2 Family & Apoptosis Regulation

  • The Bcl-2 family includes pro- and anti-apoptotic proteins (mostly transmembrane proteins).
  • Pro-apoptotic members: Bax, Bak, Bok, and others. These trigger apoptosis.
  • Anti-apoptotic members: Bcl-2, Bcl-xL, Bcl-w, and others. These inhibit apoptosis.
  • Binding of extracellular trophic factors can trigger changes in interactions between pro- and anti-apoptotic proteins, resulting in cell survival.
  • DNA Damage triggers Puma gene synthesis, which binds to Bcl-2 and promotes Bax-Bax pore formation, initiating apoptosis.

Fas-Mediated Apoptosis

  • Fas-mediated apoptosis is regulated by extracellular death signals (like tumor necrosis factor and Fas ligand) binding to their receptors. This process oligomerizes associated protein FADD (which then triggers caspase cascade) leading to apoptosis.

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