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What is the purpose of injecting ES cells that have undergone homologous recombination into a 4-day-old mouse embryo?
What is the purpose of injecting ES cells that have undergone homologous recombination into a 4-day-old mouse embryo?
What is the purpose of 'knock-out mice'?
What is the purpose of 'knock-out mice'?
What is the process of cloning by manipulation of Embryonic Stem Cells?
What is the process of cloning by manipulation of Embryonic Stem Cells?
What is the purpose of chimeric mice in this context?
What is the purpose of chimeric mice in this context?
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What is the technique used in reproductive cloning mentioned in the text?
What is the technique used in reproductive cloning mentioned in the text?
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'Unconditional KO' refers to:
'Unconditional KO' refers to:
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What is the purpose of stem cell plasticity mentioned in the text?
What is the purpose of stem cell plasticity mentioned in the text?
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What is the purpose of post-implantation genetic diagnosis mentioned in the text?
What is the purpose of post-implantation genetic diagnosis mentioned in the text?
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What does SCNT stand for?
What does SCNT stand for?
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'Chimeric Mice' are used for:
'Chimeric Mice' are used for:
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What is the purpose of isolating ES cells from 'inner cell mass' of embryos?
What is the purpose of isolating ES cells from 'inner cell mass' of embryos?
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Which type of stem cell can form any cell of the embryo?
Which type of stem cell can form any cell of the embryo?
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Which stem cell type can differentiate into cells derived from the three germ cell layers?
Which stem cell type can differentiate into cells derived from the three germ cell layers?
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Which type of stem cell can produce cells of a closely related family of cells, such as T & B cells, NK cells?
Which type of stem cell can produce cells of a closely related family of cells, such as T & B cells, NK cells?
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Which type of stem cell only produces one cell type but has the property of self-renewal?
Which type of stem cell only produces one cell type but has the property of self-renewal?
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What is the advantage of embryonic stem cells over adult stem cells?
What is the advantage of embryonic stem cells over adult stem cells?
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What is the advantage of adult stem cells over embryonic stem cells?
What is the advantage of adult stem cells over embryonic stem cells?
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What are the main components of the haemopoietic 'niche' as mentioned in the text?
What are the main components of the haemopoietic 'niche' as mentioned in the text?
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Which factors are required for the maintenance of stem cells within their niche in the bone marrow?
Which factors are required for the maintenance of stem cells within their niche in the bone marrow?
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What is the function of the haemopoietic 'niche' mentioned in the text?
What is the function of the haemopoietic 'niche' mentioned in the text?
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What is the main property of stem cells mentioned in the text?
What is the main property of stem cells mentioned in the text?
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Which of the following factors is required for maintenance and retention of stem cells within their niche in the bone marrow?
Which of the following factors is required for maintenance and retention of stem cells within their niche in the bone marrow?
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What are the end products of Hematopoiesis as mentioned in the text?
What are the end products of Hematopoiesis as mentioned in the text?
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Which type of cells compose the haemopoietic 'niche'?
Which type of cells compose the haemopoietic 'niche'?
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Which factors are required for maintenance and platelet production within the stem cell niche in bone marrow?
Which factors are required for maintenance and platelet production within the stem cell niche in bone marrow?
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What is one function of the haemopoietic 'niche' as mentioned in the text?
What is one function of the haemopoietic 'niche' as mentioned in the text?
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Which property best describes stem cells according to the text?
Which property best describes stem cells according to the text?
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Totipotent stem cells can form any cell of the ______
Totipotent stem cells can form any cell of the ______
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Pluripotent stem cells can differentiate into cells derived from the three ______ cell layers
Pluripotent stem cells can differentiate into cells derived from the three ______ cell layers
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Multipotent stem cells can produce cells of a closely related family of ______
Multipotent stem cells can produce cells of a closely related family of ______
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Unipotent stem cells only produce one ______ type
Unipotent stem cells only produce one ______ type
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Blood is collected from umbilical cord immediately after ______
Blood is collected from umbilical cord immediately after ______
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The number of cells in 1 ml of cord blood is ______
The number of cells in 1 ml of cord blood is ______
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Embryonic stem cells are 'Pluripotent' and can become any ______
Embryonic stem cells are 'Pluripotent' and can become any ______
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Adult stem cells are 'Multipotent' and can become many but not any ______
Adult stem cells are 'Multipotent' and can become many but not any ______
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Stem cells have been found in the blood, bone marrow, liver, kidney, cornea, dental pulp, umbilical cord, ______, skin, muscle, salivary gland
Stem cells have been found in the blood, bone marrow, liver, kidney, cornea, dental pulp, umbilical cord, ______, skin, muscle, salivary gland
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Transgenic animals carry a foreign gene (DNA) that has been deliberately inserted into its genome ______
Transgenic animals carry a foreign gene (DNA) that has been deliberately inserted into its genome ______
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Transgenic mice are useful for characterizing the ability of a promoter to direct ______ gene expression
Transgenic mice are useful for characterizing the ability of a promoter to direct ______ gene expression
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Eggs harvested from 0.5 dpc can be transferred into pseudopregnant female oviducts for transgenic sheep ______
Eggs harvested from 0.5 dpc can be transferred into pseudopregnant female oviducts for transgenic sheep ______
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Stem cells have the ability to go through numerous cycles of cell division while maintaining the undifferentiated state, which is known as ____________
Stem cells have the ability to go through numerous cycles of cell division while maintaining the undifferentiated state, which is known as ____________
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The haemopoietic 'niche' is composed of microenvironmental cells that nurture stem cells, protect from apoptosis, prevent excessive differentiation, and prevent excessive proliferation, such as mesenchymal stromal cells, nerve cells, osteoclasts, osteoblasts, and immune cells. These cells are the main haemopoietic ____________
The haemopoietic 'niche' is composed of microenvironmental cells that nurture stem cells, protect from apoptosis, prevent excessive differentiation, and prevent excessive proliferation, such as mesenchymal stromal cells, nerve cells, osteoclasts, osteoblasts, and immune cells. These cells are the main haemopoietic ____________
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The function of the haemopoietic 'niche' includes nurturing stem cells, protecting from apoptosis, preventing excessive differentiation, and preventing excessive proliferation, ultimately maintaining the ____________
The function of the haemopoietic 'niche' includes nurturing stem cells, protecting from apoptosis, preventing excessive differentiation, and preventing excessive proliferation, ultimately maintaining the ____________
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The main components of the haemopoietic 'niche' are seeds (stem cells), soil (bone marrow 'niche'), and fertilizer (growth factors and water). These components work together to support and regulate the function of ____________
The main components of the haemopoietic 'niche' are seeds (stem cells), soil (bone marrow 'niche'), and fertilizer (growth factors and water). These components work together to support and regulate the function of ____________
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The growth factor required for maintenance and retention of stem cells within their niche in the bone marrow is known as ____________
The growth factor required for maintenance and retention of stem cells within their niche in the bone marrow is known as ____________
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The factors required for maintenance and platelet production within the stem cell niche in the bone marrow include SCF (Stem cell factor), CXCL12, thrombopoietin, TGF-Transforming growth factor beta, and other factors such as angiogenin, fibroblast growth factor (FGF), interleukin 6, and stromal cells LEPR. These factors collectively support the function of ____________
The factors required for maintenance and platelet production within the stem cell niche in the bone marrow include SCF (Stem cell factor), CXCL12, thrombopoietin, TGF-Transforming growth factor beta, and other factors such as angiogenin, fibroblast growth factor (FGF), interleukin 6, and stromal cells LEPR. These factors collectively support the function of ____________
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The ability of stem cells to differentiate into cells derived from the three germ cell layers, which are endoderm, mesoderm, and ectoderm, is known as ____________
The ability of stem cells to differentiate into cells derived from the three germ cell layers, which are endoderm, mesoderm, and ectoderm, is known as ____________
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The factors required for the maintenance of stem cells within their niche in the bone marrow include SCF (Stem cell factor), CXCL12, thrombopoietin, TGF-Transforming growth factor beta, and other factors. These factors collectively support the function of ____________
The factors required for the maintenance of stem cells within their niche in the bone marrow include SCF (Stem cell factor), CXCL12, thrombopoietin, TGF-Transforming growth factor beta, and other factors. These factors collectively support the function of ____________
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The property of stem cells that allows them to differentiate into a closely related family of cells, such as T and B cells, NK cells, is known as ____________
The property of stem cells that allows them to differentiate into a closely related family of cells, such as T and B cells, NK cells, is known as ____________
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The function of the haemopoietic 'niche' cells is to nurture stem cells, protect from apoptosis, prevent excessive differentiation, and prevent excessive proliferation. These cells collectively support the function and maintenance of ____________
The function of the haemopoietic 'niche' cells is to nurture stem cells, protect from apoptosis, prevent excessive differentiation, and prevent excessive proliferation. These cells collectively support the function and maintenance of ____________
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The main purpose of the haemopoietic 'niche' is to support and regulate the function of ____________
The main purpose of the haemopoietic 'niche' is to support and regulate the function of ____________
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The main factors required for maintenance and retention of stem cells within their niche in the bone marrow include SCF (Stem cell factor), CXCL12, thrombopoietin, TGF-Transforming growth factor beta, and other factors such as angiogenin, fibroblast growth factor (FGF), interleukin 6, and stromal cells LEPR. These factors collectively support the function of ____________
The main factors required for maintenance and retention of stem cells within their niche in the bone marrow include SCF (Stem cell factor), CXCL12, thrombopoietin, TGF-Transforming growth factor beta, and other factors such as angiogenin, fibroblast growth factor (FGF), interleukin 6, and stromal cells LEPR. These factors collectively support the function of ____________
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ES cells that have undergone homologous recombination are identified and injected into a 4 day old mouse embryo - a blastocyst – targeted insertion Medicine 1 Lecture 3 P100 - P101 Monday, December 11, 2023 Presentation title 18 Integration is by homologous recombination Medicine 1 Lecture 3 P100 - P101 Monday, December 11, 2023 Knock-out mice Used to study the physiological role of a protein of interest in growth and development by deleting it (“knocking- out”) in embryos ‘Unconditional KO’: Gene deleted in all tissues ‘Targeted KO’: gene deleted only in specific tissues (eg only heart, or brain, rather than all organs) and at different time- points (eg at birth, only after a certain age) Medicine 1 Lecture 3 P100 - P101 Monday, December 11, 2023 Presentation title 26 Cloning by manipulation of ______ Stem Cells Isolate ES cells from ‘inner cell mass’ of embryos Culture cells in vitro (these are available as cell lines for mice strains) Introduce new genes or alter existing genes in the cultures ES cells (homologous recombination) Select cells which have gene alteration – Positive selection (antibiotic) – Negative selection (gancyclovir to kill-off ‘Tk) Transfer them back into other embryos Medicine 1 Lecture 3 P100 - P101 Monday, December 11, 2023 Presentation title 28 Chimeric Mice Medicine 1 Lecture 3 P100 - P101 Monday, December 11, 2023 Presentation title 30 Reproductive Cloning: Somatic Cell Nuclear Transfer (SCNT) Pre-implantation Genetic Diagnosis Screening for gene expression and imprinting defects Enucleation Infertile Male Clhild Post-implantation Genetic Diagnosis screening for gene integrity and imprinting defects Medicine 1 Lecture 3 P100 - P101 Monday, December 11, 2023 Ethical issues Are we trying to play GOD. Is embryo a person. Will stem cell research encourage embryo destruction and abortions. Medicine 1 Lecture 3 P100 - P101 Monday, December 11, 2023 http://www.biotechnology.gov.au/assets/documents/bainte rnet/StemCells2006820060502132032.pdf Medicine 1 Lecture 3 P100 - P101 Monday, December 11, 2023 Presentation title 33 Graf T, Blood, 1 May 2002, Vol. 99, No. 9, pp. 3089-31 Summary of hematopoietic-to-nonhematopoietic cell conversions The scheme summarizes experiments in which bone marrow cells were transplanted into mice, which were then analysed several months later hematopoietic and nonhaemopoietic donor type cells. Medicine 1 Lecture 3 P100 - P101 Monday, December 11, 2023 Presentation title 34 Stem cell plasticity ASC; Adipose-derived stromal/stem cells NSC; Neural stem cells Transdetermination - a switch in lineage commitment in a stem or progenitor cell Immunological Rev 187:22-39 Transdetermination Medicine 1 Lecture 3 P100 - P101 Monday, December 11, 2023 35 Medicine 1 Lecture 3 P100 - P101 Monday, December 11, 2023 35.
ES cells that have undergone homologous recombination are identified and injected into a 4 day old mouse embryo - a blastocyst – targeted insertion Medicine 1 Lecture 3 P100 - P101 Monday, December 11, 2023 Presentation title 18 Integration is by homologous recombination Medicine 1 Lecture 3 P100 - P101 Monday, December 11, 2023 Knock-out mice Used to study the physiological role of a protein of interest in growth and development by deleting it (“knocking- out”) in embryos ‘Unconditional KO’: Gene deleted in all tissues ‘Targeted KO’: gene deleted only in specific tissues (eg only heart, or brain, rather than all organs) and at different time- points (eg at birth, only after a certain age) Medicine 1 Lecture 3 P100 - P101 Monday, December 11, 2023 Presentation title 26 Cloning by manipulation of ______ Stem Cells Isolate ES cells from ‘inner cell mass’ of embryos Culture cells in vitro (these are available as cell lines for mice strains) Introduce new genes or alter existing genes in the cultures ES cells (homologous recombination) Select cells which have gene alteration – Positive selection (antibiotic) – Negative selection (gancyclovir to kill-off ‘Tk) Transfer them back into other embryos Medicine 1 Lecture 3 P100 - P101 Monday, December 11, 2023 Presentation title 28 Chimeric Mice Medicine 1 Lecture 3 P100 - P101 Monday, December 11, 2023 Presentation title 30 Reproductive Cloning: Somatic Cell Nuclear Transfer (SCNT) Pre-implantation Genetic Diagnosis Screening for gene expression and imprinting defects Enucleation Infertile Male Clhild Post-implantation Genetic Diagnosis screening for gene integrity and imprinting defects Medicine 1 Lecture 3 P100 - P101 Monday, December 11, 2023 Ethical issues Are we trying to play GOD. Is embryo a person. Will stem cell research encourage embryo destruction and abortions. Medicine 1 Lecture 3 P100 - P101 Monday, December 11, 2023 http://www.biotechnology.gov.au/assets/documents/bainte rnet/StemCells2006820060502132032.pdf Medicine 1 Lecture 3 P100 - P101 Monday, December 11, 2023 Presentation title 33 Graf T, Blood, 1 May 2002, Vol. 99, No. 9, pp. 3089-31 Summary of hematopoietic-to-nonhematopoietic cell conversions The scheme summarizes experiments in which bone marrow cells were transplanted into mice, which were then analysed several months later hematopoietic and nonhaemopoietic donor type cells. Medicine 1 Lecture 3 P100 - P101 Monday, December 11, 2023 Presentation title 34 Stem cell plasticity ASC; Adipose-derived stromal/stem cells NSC; Neural stem cells Transdetermination - a switch in lineage commitment in a stem or progenitor cell Immunological Rev 187:22-39 Transdetermination Medicine 1 Lecture 3 P100 - P101 Monday, December 11, 2023 35 Medicine 1 Lecture 3 P100 - P101 Monday, December 11, 2023 35.
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