Podcast
Questions and Answers
Which stain is commonly used for the demonstration of bacterial organisms?
Which stain is commonly used for the demonstration of bacterial organisms?
What does the addition of Gram's iodine do in the staining process?
What does the addition of Gram's iodine do in the staining process?
What color does Gram-positive organism show after acetone treatment in Gram's staining?
What color does Gram-positive organism show after acetone treatment in Gram's staining?
Which stain is commonly used for acid-fast staining of bacteria?
Which stain is commonly used for acid-fast staining of bacteria?
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What is the purpose of using carbol-fuchsin in acid-fast staining?
What is the purpose of using carbol-fuchsin in acid-fast staining?
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Study Notes
Bacterial Staining Techniques
- Common Stain for Bacteria: Gram stain is the primary method used for demonstrating bacterial organisms, allowing differentiation between Gram-positive and Gram-negative bacteria.
- Role of Gram's Iodine: Acts as a mordant in the Gram staining process, enhancing the binding of crystal violet dye to the bacterial cell wall, improving the retention of color during rinsing.
- Color Change in Gram-positive Bacteria: After treatment with acetone, Gram-positive organisms typically retain the crystal violet stain and appear purple, indicating their thick peptidoglycan layer.
- Stain for Acid-Fast Bacteria: Ziehl-Neelsen stain, which is essential for identifying acid-fast bacilli (AFB), most notably Mycobacterium tuberculosis.
- Function of Carbol-Fuchsin: Used as the primary stain in acid-fast staining; it penetrates the waxy cell wall of acid-fast bacteria, allowing them to retain the red color even after decolorization.
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Description
Test your knowledge about the special stains used to identify microbial organisms in tissue or smear samples. Learn about the different staining techniques that help delineate the morphology and characteristic color of bacteria, fungi, protozoa, helminths, and viruses.
