Spirochetes L1

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Questions and Answers

Key facts regarding Spirochetes: morphologically they are ____ or _____ bacteria

Spiral or helical

Spirochetes are gram + bacteria

False (B)

Spirochetes stain poorly despite being gram -

True (A)

What type of stain is used to identify spirochetes?

<p>Silver stains</p> Signup and view all the answers

What environment to spirochetes grow and survive?

<p>Moist conditions</p> Signup and view all the answers

The motility of spirochetes are related to their capacity of ______ their host, cause _____ or ______ of the host.

<p>Infecting, lesions or pathogenesis</p> Signup and view all the answers

What is one of the key characteristic of spirochetes?

<p>Some are commensals, but many cause zoonotic infections (B)</p> Signup and view all the answers

What types of infections can spirochetes cause?

<p>Systemic or intestinal infections (C)</p> Signup and view all the answers

What method is commonly used for epidemiological investigations and clinical diagnosis of spirochetes?

<p>Serological methods (C)</p> Signup and view all the answers

How are spirochetes classified?

<p>Based on genetic relatedness (C)</p> Signup and view all the answers

What are the three genuses of veterinary importance ?

<p>Leptospirosis, borrelia and brachyspira</p> Signup and view all the answers

Borrelia grow very quickly in specialized media

<p>False (B)</p> Signup and view all the answers

Which of the following is Borrelia?

<p>C (C)</p> Signup and view all the answers

Which one is Leptospira?

<p>A (A)</p> Signup and view all the answers

Which of the following is Brachyspira?

<p>B (B)</p> Signup and view all the answers

How is borrelia transmitted?

<p>Transmitted by arthropod vectors</p> Signup and view all the answers

Borrelia is capable of surviving well in the environment.

<p>False (B)</p> Signup and view all the answers

Where can Borrelia be found if it is incapable of surviving in the environment?

<p>Either inside the vector or a host. (An animal or human host)</p> Signup and view all the answers

How is Borrelia maintained in nature?

<p>In a two-year enzootic life cycle involving ticks and mammals (D)</p> Signup and view all the answers

Which type of tick is primarily responsible for transmitting Borrelia in North America?

<p>Ixodid ticks (B)</p> Signup and view all the answers

What role does the white-footed mouse play in the Borrelia life cycle?

<p>It serves as a reservoir host, allowing ticks to acquire the bacteria (A)</p> Signup and view all the answers

What is the role of deer in the Borrelia life cycle?

<p>They do not get infected but help in tick reproduction and dispersion (C)</p> Signup and view all the answers

Which of the following statements is TRUE about Borrelia transmission?

<p>The bacteria are passed to ticks when they feed on an infected white-footed mouse (D)</p> Signup and view all the answers

Borrelia can cause disease in which of the following groups?

<p>Birds, mammals, and humans (C)</p> Signup and view all the answers

Which of the following are the two species of Borrelia that cause disease? More than one may apply

<p>relapsing fever borreliae (A), Lyme borreliosis (D)</p> Signup and view all the answers

Lyme borreliosis will be caused by the species: ________

<p>B. Burgodorferi sense lato</p> Signup and view all the answers

B. Burgodorferi sense lato is a group of 13 species. The one we are focusing on the agent of Lyme in North America, and this species is:

<p>B. Burgdorferi sensu stricto (C)</p> Signup and view all the answers

B. Burgdorferi will be infecting the reservoir hosts that were transmitted by ticks. Which of the following are the reservoir hosts?

<p>All the above (D)</p> Signup and view all the answers

What are the clinical conditions that B. Burgdoferi cause?

<p>Arthritis, neurological and cardiac disease</p> Signup and view all the answers

Which mammals will be normally see arthritic, neurological and cardiac disease in?

<p>Humans and dogs (A)</p> Signup and view all the answers

Which mammals will we occasionally see arthritic, neurological and cardiac disease in?

<p>All the above (D)</p> Signup and view all the answers

What species of ticks is responsible for the Lyme borrelia ( B. Burgdorferi sensu stricto)

<p>Ixodes scapularis (A)</p> Signup and view all the answers

In which species has Lyme Borreliosis been reported?

<p>Humans, dogs, horses, cattle, and sheep (C)</p> Signup and view all the answers

Where and when was Lyme disease first identified?

<p>1975 in Old Lyme, Connecticut (B)</p> Signup and view all the answers

What is the initial location of Borrelia in an unfed tick?

<p>Midgut (B)</p> Signup and view all the answers

How long does a tick need to be attached for Borrelia transmission to occur?

<p>24-48 hours (C)</p> Signup and view all the answers

After entering the host, where does Borrelia burgdorferi first multiply?

<p>Skin (C)</p> Signup and view all the answers

What is the typical incubation period for Lyme disease?

<p>2-6 months (C)</p> Signup and view all the answers

What is the function of OspF in Borrelia burgdorferi?

<p>Protects bacteria inside the host (B)</p> Signup and view all the answers

What is the function of OspA ?

<p>This surface protein aids in the adherence to the gut of the tick (A)</p> Signup and view all the answers

What is the function of OspC?

<p>Protects the borreliae in tick saliva and in early stages of disease on a host (D)</p> Signup and view all the answers

What are the common clinical signs of Lyme borreliosis in humans?

<p>Flu-like illness and skin rash (B)</p> Signup and view all the answers

What serious complications can Lyme disease cause in humans?

<p>Arthritis and permanent nerve damage (A)</p> Signup and view all the answers

Which of the following statements about Lyme disease in dogs is true?

<p>Clinical signs include arthritis, lameness, fever, and lethargy (C)</p> Signup and view all the answers

In horses, Lyme disease can cause all of the following EXCEPT:

<p>Severe gastrointestinal ulcers (C)</p> Signup and view all the answers

What is required for a clinical diagnosis of Lyme disease?

<p>Exposure to ticks in endemic areas + clinical signs (A)</p> Signup and view all the answers

Which diagnostic test is considered confirmatory for Lyme disease?

<p>Culture of Borrelia from affected animals (C)</p> Signup and view all the answers

Why is PCR not useful for Lyme disease diagnosis in animals?

<p>There are usually low loads of DNA (C)</p> Signup and view all the answers

What is the purpose of the multiplex ELISA for Lyme disease in dogs and horses?

<p>Detects multiple Borrelia outer surface proteins (OspA, OspC, OspF) (A)</p> Signup and view all the answers

Why is Multiplex a good test for dogs and horses?

<p>You can say if the animal is infected, if there’s only vaccination and the stage of the disease if they are infected.</p> Signup and view all the answers

Match the test results of the Multiplex Elisa to it’s best description

<p>If animal is OSPA + = Indicates only vaccination If only OSPC + = It is early infection because this is when the tick is feeding If have OSPC + and OSPF + = Indicates an intermediate infection Only OSPF + (so OSPC and OSPA -) = Indicates a late infection</p> Signup and view all the answers

Which antibiotics are recommended for treating acute Lyme disease in dogs?

<p>Amoxicillin and oxytetracycline (B)</p> Signup and view all the answers

How is chronic Lyme disease treated in dogs?

<p>Prolonged or repeated courses of antibiotics</p> Signup and view all the answers

Flashcards

Spirochetes morphology

Spiral or helical bacteria

Spirochetes are gram + bacteria

False, spirochetes are gram-negative bacteria.

Spirochetes stain poorly despite gram -

True, spirochetes are gram - but stain poorly.

Stain to identify spirochetes

Silver stains.

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Environment for spirochetes to grow

Moist conditions.

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Motility of spirochetes related to

Infecting their host, cause lesions or pathogenesis of the host.

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Key characteristic of spirochetes

Some are commensals, but many cause zoonotic infections.

Signup and view all the flashcards

Types of infections spirochetes cause

Systemic or intestinal infections.

Signup and view all the flashcards

Common method for spirochetes investigations

Serological methods.

Signup and view all the flashcards

How spirochetes are classified

Based on genetic relatedness.

Signup and view all the flashcards

Three genuses of veterinary importance

Leptospirosis, Borrelia, and Brachyspira.

Signup and view all the flashcards

Borrelia grow very quickly in specialized media

False.

Signup and view all the flashcards

How is Borrelia transmitted?

Transmitted by arthropod vectors.

Signup and view all the flashcards

Borrelia is capable of surviving well in the environment

False.

Signup and view all the flashcards

Where can Borrelia be found?

Either inside the vector or a host (an animal or human host).

Signup and view all the flashcards

How is Borrelia maintained in nature?

In a two-year enzootic life cycle involving ticks and mammals.

Signup and view all the flashcards

Tick primarily for transmitting Borrelia

Ixodid ticks.

Signup and view all the flashcards

Role of the white-footed mouse

It serves as a reservoir host, allowing ticks to acquire the bacteria.

Signup and view all the flashcards

Role of deer

They do not get infected but help in tick reproduction and dispersion.

Signup and view all the flashcards

Which statement is TRUE about Borrelia transmission?

The bacteria are passed to ticks when they feed on an infected white-footed mouse.

Signup and view all the flashcards

Borrelia can cause disease in which?

Birds, mammals, and humans.

Signup and view all the flashcards

Two Borrelia species that cause disease

Relapsing fever borreliae and Lyme borreliosis borreliae

Signup and view all the flashcards

Lyme borreliosis will be caused by the species: .

B. Burgdorferi sense lato.

Signup and view all the flashcards

Agent of Lyme in North America

B. Burgdorferi sensu stricto.

Signup and view all the flashcards

Reservoir hosts for B. Burgdorferi

White footed mice

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Clinical conditions that B. Burgdoferi cause

Arthritis, neurological and cardiac disease.

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Mammals normally see disease in

Humans and dogs.

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Tick species responsible for Lyme borrelia

Ixodes scapularis.

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When and where was Lyme disease first identified?

1975 in Old Lyme, Connecticut.

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Initial location of Borrelia

Midgut.

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