30 Questions
What is the primary focus of the branch of science known as spectroscopy?
Studying the interaction of matter with light or electromagnetic radiation
What happens when electromagnetic radiation interacts with matter?
The electric and magnetic dipole moment in the molecule or nuclear spin changes
What is the total energy of a molecule comprised of?
Translational, rotational, vibrational, and electronic energy
What is the result of absorption or emission of electromagnetic radiation in molecular spectroscopy?
A change in any of the energy states of the molecule
Why is there no spectroscopy for translational energy?
Because energy levels are not quantized
What is the primary application of spectroscopy?
Analyzing the structure of atoms and molecules
What type of energy is due to spinning of molecules about the axis passing through the centre of gravity?
Rotational energy
Which type of spectroscopy is used to study vibrational energy?
IR spectroscopy
What is the purpose of a monochromator in UV-Visible spectroscopy?
To resolve polychromatic radiation into its individual wavelengths
Which type of lamp is commonly used for the visible range in UV-Visible spectroscopy?
Tungsten filament lamp
What is the principle of spectroscopy based on?
The measurement of the spectrum of a sample containing atoms/molecules
What type of prism is used for the UV range in UV-Visible spectroscopy?
Fused silica prism
What is the primary consideration for selecting a sample holder in UV-visible spectroscopy?
The sample holder should be transparent to UV-visible radiation
What is the purpose of selecting a specific solvent in UV-visible spectroscopy?
To prevent the solvent from absorbing in the UV-visible range
What is the role of detectors in UV-visible spectroscopy?
To detect the transmitted radiation
What is the relationship between absorbance A and %T?
A = 2-log10(%T)
What is the process of absorbing light at a certain wavelength and emitting at another wavelength?
Fluorescence
What is the typical direction of incident light and emitted light in fluorescence spectroscopy?
Incident light from one direction and emitted light in all directions
What is the primary mechanism of fluorescence?
Molecules raising to an electronic excited state due to photons
What is the purpose of analyzing the emission and excitation spectra in fluorescence spectroscopy?
To see how a sample is changing due to external factors
What is a common application of fluorescence spectroscopy in environmental monitoring?
Analyzing water samples for impurities
What is a characteristic of some fluorophores that can be used in fluorescence spectroscopy?
They are sensitive to solvent environment properties
What is a potential use of fluorescence spectroscopy in the pharmaceutical industry?
Detecting impurities in pharmaceutical products
What is a potential biomedical application of fluorescence spectroscopy?
Cancer diagnostics in human tissues
What is the energy transfer ΔE possible between in a nucleus when an external magnetic field is applied?
Ground state to excited state
What is the purpose of the sweep generator in NMR instrumentation?
To produce an equal amount of magnetic field through the sample
What is the direct proportionality of the emitted radio frequency in NMR spectroscopy?
Strength of the applied field
What is the purpose of the permanent magnet in NMR instrumentation?
To provide a homogeneous magnetic field
What is the plot of in an NMR spectrum?
Intensity of NMR signals vs magnetic field
What is the role of the radio receiver coil in NMR instrumentation?
To detect the radio frequencies emitted as nuclei relax
Test your understanding of spectroscopy, a branch of science that deals with the interaction of matter with light or electromagnetic radiation. This quiz covers the techniques and applications of spectroscopy, including the measurement of signals as a function of wavelength.
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