Questions and Answers
What is the primary purpose of UV-Vis Spectroscopy?
Which type of spectroscopy is used to determine the structure of molecules?
What is the primary purpose of Chromatography?
Which type of Chromatography separates components based on their boiling points and affinity for a stationary phase?
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What is the application of Spectroscopy in industries?
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What is the application of Chromatography in pharmaceuticals?
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Which type of Spectroscopy measures the absorption of infrared radiation by molecules?
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What is the application of Mass Spectroscopy?
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Which type of Chromatography uses high pressure and a stationary phase to separate components?
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What is the application of Thin Layer Chromatography (TLC)?
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Study Notes
Spectroscopy
Definition
Spectroscopy is an analytical technique used to measure the interaction between matter and electromagnetic radiation.
Types of Spectroscopy
- UV-Vis Spectroscopy: measures the absorption of UV and visible light by molecules, used to determine the concentration of a substance.
- IR Spectroscopy: measures the absorption of infrared radiation by molecules, used to identify functional groups in a molecule.
- NMR Spectroscopy: measures the interaction between atomic nuclei and magnetic fields, used to determine the structure of molecules.
- Mass Spectroscopy: measures the mass-to-charge ratio of ions, used to identify and quantify molecules.
Applications
- Identification and quantification of molecules
- Determination of molecular structure
- Analysis of chemical reactions
- Quality control in industries
Chromatography
Definition
Chromatography is a separation technique used to separate, identify, and quantify the components of a mixture.
Types of Chromatography
- Liquid Chromatography (LC): separates components based on their interaction with a stationary phase and a mobile phase.
- Gas Chromatography (GC): separates components based on their boiling points and affinity for a stationary phase.
- Thin Layer Chromatography (TLC): separates components based on their affinity for a stationary phase and a mobile phase.
- High-Performance Liquid Chromatography (HPLC): a type of LC that uses high pressure and a stationary phase to separate components.
Applications
- Separation and purification of mixtures
- Identification and quantification of components
- Analysis of pharmaceuticals and biological samples
- Quality control in industries
Spectroscopy
Definition
- Measures interaction between matter and electromagnetic radiation
Types of Spectroscopy
- UV-Vis Spectroscopy: measures absorption of UV and visible light, determines concentration of a substance
- IR Spectroscopy: measures absorption of infrared radiation, identifies functional groups in a molecule
- NMR Spectroscopy: measures interaction between atomic nuclei and magnetic fields, determines molecular structure
- Mass Spectroscopy: measures mass-to-charge ratio of ions, identifies and quantifies molecules
Applications
- Identifies and quantifies molecules
- Determines molecular structure
- Analyzes chemical reactions
- Ensures quality control in industries
Chromatography
Definition
- Separates, identifies, and quantifies components of a mixture
Types of Chromatography
- Liquid Chromatography (LC): separates components based on interaction with stationary and mobile phases
- Gas Chromatography (GC): separates components based on boiling points and affinity for a stationary phase
- Thin Layer Chromatography (TLC): separates components based on affinity for a stationary phase and a mobile phase
- High-Performance Liquid Chromatography (HPLC): uses high pressure and a stationary phase to separate components
Applications
- Separates and purifies mixtures
- Identifies and quantifies components
- Analyzes pharmaceuticals and biological samples
- Ensures quality control in industries
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Description
Learn about spectroscopy, an analytical technique used to measure the interaction between matter and electromagnetic radiation. Explore types of spectroscopy, including UV-Vis, IR, and NMR.
