6 Questions
What is spectroscopy?
Spectroscopy is the use of electromagnetic radiation to investigate a substance qualitatively or quantitatively, or to examine physical processes.
What is a spectrophotometer?
A spectrophotometer is a device that counts the number of photons absorbed by a sample solution after passing through it, and estimates the quantities of a given chemical compound by measuring the intensity of light detected.
What is the difference between absorption and emission spectroscopy?
In absorption spectroscopy, light energy is used to excite matter from ground state to excited energy state, while in emission spectroscopy, matter in an excited energy state emits light radiation and returns to the ground level energy state. Non-radiative decay occurs if no radiation is emitted during the transition from higher to lower energy levels.
Which of the following is the principle of spectroscopy?
Investigating a substance with electromagnetic radiation
What is the difference between absorption and emission spectroscopy?
Absorption measures the amount of radiation absorbed, while emission measures the amount of radiation emitted
What instruments are used to measure the magnitude of light radiations absorbed or emitted by matter in spectroscopic techniques?
Spectrophotometer and fluorometer
Study Notes
Spectroscopy Basics
- Spectroscopy is the study of the interaction between matter and electromagnetic radiation, which involves the absorption, emission, or scattering of radiation by atoms or molecules.
Spectrophotometer
- A spectrophotometer is an instrument used to measure the amount of light absorbed or transmitted by a sample, typically in the ultraviolet, visible, or infrared regions of the electromagnetic spectrum.
Absorption and Emission Spectroscopy
- Absorption spectroscopy involves the measurement of the amount of radiation absorbed by a sample at different wavelengths, resulting in the absorption spectrum.
- Emission spectroscopy involves the measurement of the radiation emitted by a sample at different wavelengths, resulting in the emission spectrum.
- The main difference between absorption and emission spectroscopy lies in the direction of energy flow: absorption spectroscopy involves the absorption of radiation by a sample, while emission spectroscopy involves the emission of radiation by a sample.
Principle of Spectroscopy
- The principle of spectroscopy is based on the idea that atoms and molecules absorb and emit radiation at specific wavelengths, which can be used to identify and quantify the presence of a particular substance.
Instruments Used in Spectroscopy
- Spectrophotometers, spectrographs, and spectrometers are instruments used to measure the magnitude of light radiations absorbed or emitted by matter in spectroscopic techniques.
Test your knowledge of spectroscopy and spectrophotometry with this quiz! Learn about the basic definitions, principles, and applications of these analytical techniques. Challenge yourself to identify the differences between the two and understand how they are used in scientific research. Keywords: spectroscopy, spectrophotometer, electromagnetic radiation, photons, sample solution.
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