22 Questions
Which type of radiation is spectrophotometry most commonly applied to?
Visible
What are some important features of spectrophotometers?
All of the above
What can a spectrophotometer measure?
All of the above
Which company was responsible for creating the first commercially available diode-array spectrophotometer?
Hewlett-Packard
What is the purpose of using spectrophotometry in the determination of the equilibrium constant of a solution?
To measure the concentration of reactants and products at the equilibrium point
Which model of spectrophotometer was the most popular and widely recognized?
Model D
What is the role of a spectrophotometer in astronomy?
To measure the spectrum of a celestial object
Which type of spectrophotometer measures the relative light intensity of the beam before and after a test sample is inserted?
Single-beam spectrophotometer
Which type of spectrophotometer compares the light intensity between two light paths, one path containing a reference sample and the other the test sample?
Double-beam spectrophotometer
Which type of spectrophotometer uses a monochromator containing a diffraction grating to produce the analytical spectrum?
Scanning spectrophotometer
Which type of spectrophotometer uses a Fourier transform technique to acquire the spectral information?
Array spectrophotometer
Which type of electromagnetic radiation is spectrophotometry commonly applied to?
Visible
What is the range of wavelengths that spectrophotometers can measure?
200-2500 nm
What are some important features of spectrophotometers?
Spectral bandwidth, percentage of sample-transmission, logarithmic range of sample-absorption
Which company was responsible for creating the first commercially available spectrophotometer?
Hewlett-Packard
What is the role of a spectrophotometer in the determination of the equilibrium constant of a solution?
To measure the concentration of reactants and products at the equilibrium point
Which type of spectrophotometer uses a monochromator containing a diffraction grating to produce the analytical spectrum?
Fourier transform spectrophotometer
What is the most important instrument ever developed towards the advancement of bioscience?
Model D spectrophotometer
Which type of spectrophotometer measures the relative light intensity of the beam before and after a test sample is inserted?
Single-beam spectrophotometer
What is the advantage of using a single-beam spectrophotometer?
All of the above
What type of spectrophotometer is commonly used in specialized instruments like microscopes or telescopes?
Single-beam spectrophotometer
What technique is commonly used in modern mid-infrared spectrophotometers to acquire spectral information?
Fourier transform technique
Study Notes
Spectrophotometry Basics
- Spectrophotometry is most commonly applied to visible and ultraviolet radiation.
- The range of wavelengths that spectrophotometers can measure is from 190 nm to 1100 nm.
Spectrophotometer Features
- Important features of spectrophotometers include high accuracy, high sensitivity, and ability to measure small sample volumes.
- Spectrophotometers can measure absorbance, transmittance, and reflectance of a sample.
Types of Spectrophotometers
- Single-beam spectrophotometers measure the relative light intensity of the beam before and after a test sample is inserted.
- Double-beam spectrophotometers compare the light intensity between two light paths, one path containing a reference sample and the other the test sample.
- Diode-array spectrophotometers use a monochromator containing a diffraction grating to produce the analytical spectrum.
- Fourier transform spectrophotometers use a Fourier transform technique to acquire the spectral information.
History of Spectrophotometers
- The first commercially available spectrophotometer was created by Hilger and Watts.
- The first commercially available diode-array spectrophotometer was created by Hewlett-Packard.
Applications of Spectrophotometers
- Spectrophotometry is used to determine the equilibrium constant of a solution by measuring the absorbance of a sample at different wavelengths.
- Spectrophotometers play a crucial role in astronomy, enabling the analysis of the composition of celestial objects.
- The most important instrument ever developed towards the advancement of bioscience is the spectrophotometer.
Advantages and Techniques
- The advantage of using a single-beam spectrophotometer is its simplicity and lower cost.
- Fourier transform technique is commonly used in modern mid-infrared spectrophotometers to acquire spectral information.
- Spectrophotometers are commonly used in specialized instruments like microscopes or telescopes.
Test your knowledge of spectrophotometry with this quiz! Learn about the principles, techniques, and applications of this branch of electromagnetic spectroscopy. Gauge your understanding of spectrophotometers and their ability to measure light intensity at different wavelengths. Challenge yourself with questions on ultraviolet and other aspects of spectrophotometry.
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