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Questions and Answers
Which type of radiation is spectrophotometry most commonly applied to?
Which type of radiation is spectrophotometry most commonly applied to?
- Visible (correct)
- Infrared
- Microwave
- Ultraviolet
What are some important features of spectrophotometers?
What are some important features of spectrophotometers?
- Spectral bandwidth
- Percentage of sample-transmission
- Logarithmic range of sample-absorption
- All of the above (correct)
What can a spectrophotometer measure?
What can a spectrophotometer measure?
- Transmittance of solutions
- Reflectance of solutions
- Transmittance of gases
- All of the above (correct)
Which company was responsible for creating the first commercially available diode-array spectrophotometer?
Which company was responsible for creating the first commercially available diode-array spectrophotometer?
What is the purpose of using spectrophotometry in the determination of the equilibrium constant of a solution?
What is the purpose of using spectrophotometry in the determination of the equilibrium constant of a solution?
Which model of spectrophotometer was the most popular and widely recognized?
Which model of spectrophotometer was the most popular and widely recognized?
What is the role of a spectrophotometer in astronomy?
What is the role of a spectrophotometer in astronomy?
Which type of spectrophotometer measures the relative light intensity of the beam before and after a test sample is inserted?
Which type of spectrophotometer measures the relative light intensity of the beam before and after a test sample is inserted?
Which type of spectrophotometer compares the light intensity between two light paths, one path containing a reference sample and the other the test sample?
Which type of spectrophotometer compares the light intensity between two light paths, one path containing a reference sample and the other the test sample?
Which type of spectrophotometer uses a monochromator containing a diffraction grating to produce the analytical spectrum?
Which type of spectrophotometer uses a monochromator containing a diffraction grating to produce the analytical spectrum?
Which type of spectrophotometer uses a Fourier transform technique to acquire the spectral information?
Which type of spectrophotometer uses a Fourier transform technique to acquire the spectral information?
Which type of electromagnetic radiation is spectrophotometry commonly applied to?
Which type of electromagnetic radiation is spectrophotometry commonly applied to?
What is the range of wavelengths that spectrophotometers can measure?
What is the range of wavelengths that spectrophotometers can measure?
What are some important features of spectrophotometers?
What are some important features of spectrophotometers?
Which company was responsible for creating the first commercially available spectrophotometer?
Which company was responsible for creating the first commercially available spectrophotometer?
What is the role of a spectrophotometer in the determination of the equilibrium constant of a solution?
What is the role of a spectrophotometer in the determination of the equilibrium constant of a solution?
Which type of spectrophotometer uses a monochromator containing a diffraction grating to produce the analytical spectrum?
Which type of spectrophotometer uses a monochromator containing a diffraction grating to produce the analytical spectrum?
What is the most important instrument ever developed towards the advancement of bioscience?
What is the most important instrument ever developed towards the advancement of bioscience?
Which type of spectrophotometer measures the relative light intensity of the beam before and after a test sample is inserted?
Which type of spectrophotometer measures the relative light intensity of the beam before and after a test sample is inserted?
What is the advantage of using a single-beam spectrophotometer?
What is the advantage of using a single-beam spectrophotometer?
What type of spectrophotometer is commonly used in specialized instruments like microscopes or telescopes?
What type of spectrophotometer is commonly used in specialized instruments like microscopes or telescopes?
What technique is commonly used in modern mid-infrared spectrophotometers to acquire spectral information?
What technique is commonly used in modern mid-infrared spectrophotometers to acquire spectral information?
Flashcards
Visible Radiation
Visible Radiation
Spectrophotometry commonly uses this radiation type.
Spectrophotometer Features
Spectrophotometer Features
Measures spectral bandwidth, sample transmission (%), and absorption range.
Spectrophotometer Measurements
Spectrophotometer Measurements
Measures absorbance, transmittance, and reflectance of a sample.
Hewlett-Packard
Hewlett-Packard
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Spectrophotometry in Equilibrium
Spectrophotometry in Equilibrium
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Model D Spectrophotometer
Model D Spectrophotometer
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Spectrophotometer in Astronomy
Spectrophotometer in Astronomy
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Single-Beam Spectrophotometer
Single-Beam Spectrophotometer
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Double-Beam Spectrophotometer
Double-Beam Spectrophotometer
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Scanning Spectrophotometer
Scanning Spectrophotometer
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Array Spectrophotometer
Array Spectrophotometer
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Spectrophotometer Wavelength Range
Spectrophotometer Wavelength Range
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Fourier Transform Spectrophotometer
Fourier Transform Spectrophotometer
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Model D Spectrophotometer
Model D Spectrophotometer
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Single-beam spectrophotometer
Single-beam spectrophotometer
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Single-Beam Spectrophotometer Advantages
Single-Beam Spectrophotometer Advantages
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Single-Beam Spectrophotometer Use
Single-Beam Spectrophotometer Use
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Fourier Transform Technique
Fourier Transform Technique
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Study Notes
Spectrophotometry Basics
- Spectrophotometry is most commonly applied to visible and ultraviolet radiation.
- The range of wavelengths that spectrophotometers can measure is from 190 nm to 1100 nm.
Spectrophotometer Features
- Important features of spectrophotometers include high accuracy, high sensitivity, and ability to measure small sample volumes.
- Spectrophotometers can measure absorbance, transmittance, and reflectance of a sample.
Types of Spectrophotometers
- Single-beam spectrophotometers measure the relative light intensity of the beam before and after a test sample is inserted.
- Double-beam spectrophotometers compare the light intensity between two light paths, one path containing a reference sample and the other the test sample.
- Diode-array spectrophotometers use a monochromator containing a diffraction grating to produce the analytical spectrum.
- Fourier transform spectrophotometers use a Fourier transform technique to acquire the spectral information.
History of Spectrophotometers
- The first commercially available spectrophotometer was created by Hilger and Watts.
- The first commercially available diode-array spectrophotometer was created by Hewlett-Packard.
Applications of Spectrophotometers
- Spectrophotometry is used to determine the equilibrium constant of a solution by measuring the absorbance of a sample at different wavelengths.
- Spectrophotometers play a crucial role in astronomy, enabling the analysis of the composition of celestial objects.
- The most important instrument ever developed towards the advancement of bioscience is the spectrophotometer.
Advantages and Techniques
- The advantage of using a single-beam spectrophotometer is its simplicity and lower cost.
- Fourier transform technique is commonly used in modern mid-infrared spectrophotometers to acquire spectral information.
- Spectrophotometers are commonly used in specialized instruments like microscopes or telescopes.
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