Spectrophotometry Essentials Quiz
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Questions and Answers

What are spectrophotometers used to measure?

  • Viscosity of a liquid
  • Electrical conductivity of a substance
  • Intensity of a light beam at different wavelengths (correct)
  • Density of a material
  • What is spectrophotometry concerned with?

  • Qualitative analysis of colored compounds
  • Measurement of radioactivity in materials
  • Analysis of molecular structures
  • Quantitative measurement of reflection or transmission properties of a material as a function of wavelength (correct)
  • Which electromagnetic radiations are commonly applied in spectrophotometry?

  • Infrared, microwave, and radio wave radiation
  • Ultraviolet, visible, and infrared radiation (correct)
  • Ultraviolet, gamma, and microwave radiation
  • X-ray, gamma, and microwave radiation
  • What are important features of spectrophotometers?

    <p>Spectral bandwidth, percentage of sample-transmission, logarithmic range of sample-absorption</p> Signup and view all the answers

    What does spectrophotometry depend on for the quantitative analysis of molecules?

    <p>How much light is absorbed by colored compounds</p> Signup and view all the answers

    Study Notes

    Spectrophotometry Basics

    • Spectrophotometers are used to measure the amount of light absorbed or transmitted by a sample.
    • Spectrophotometry is concerned with the interaction between matter and electromagnetic radiation.

    Applied Electromagnetic Radiations

    • Commonly applied electromagnetic radiations in spectrophotometry include ultraviolet (UV), visible (Vis), and infrared (IR) light.

    Spectrophotometer Features

    • Important features of spectrophotometers include accuracy, precision, and sensitivity.
    • They also have a monochromator to select specific wavelengths of light.
    • A detector is used to measure the intensity of light transmitted through or absorbed by the sample.

    Quantitative Analysis

    • Spectrophotometry depends on the Beer-Lambert law for the quantitative analysis of molecules.
    • The Beer-Lambert law relates the absorbance of light to the concentration of the sample.
    • It is a linear relationship, making it possible to calculate the concentration of a sample based on its absorbance.

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    Description

    Test your knowledge of spectrophotometry with this quiz! From the principles of electromagnetic spectroscopy to the use of spectrophotometers, this quiz covers the essentials of quantitative measurement of light reflection and transmission properties.

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