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Questions and Answers
What are spectrophotometers used to measure?
What is spectrophotometry concerned with?
Which electromagnetic radiations are commonly applied in spectrophotometry?
What are important features of spectrophotometers?
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What does spectrophotometry depend on for the quantitative analysis of molecules?
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Study Notes
Spectrophotometry Basics
- Spectrophotometers are used to measure the amount of light absorbed or transmitted by a sample.
- Spectrophotometry is concerned with the interaction between matter and electromagnetic radiation.
Applied Electromagnetic Radiations
- Commonly applied electromagnetic radiations in spectrophotometry include ultraviolet (UV), visible (Vis), and infrared (IR) light.
Spectrophotometer Features
- Important features of spectrophotometers include accuracy, precision, and sensitivity.
- They also have a monochromator to select specific wavelengths of light.
- A detector is used to measure the intensity of light transmitted through or absorbed by the sample.
Quantitative Analysis
- Spectrophotometry depends on the Beer-Lambert law for the quantitative analysis of molecules.
- The Beer-Lambert law relates the absorbance of light to the concentration of the sample.
- It is a linear relationship, making it possible to calculate the concentration of a sample based on its absorbance.
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Description
Test your knowledge of spectrophotometry with this quiz! From the principles of electromagnetic spectroscopy to the use of spectrophotometers, this quiz covers the essentials of quantitative measurement of light reflection and transmission properties.
