Spectrophotometer Basics
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Questions and Answers

What is the purpose of accreditation?

  • Provide reliable test results
  • To issue licenses to individuals
  • To certify private organizations
  • To evaluate and recognize a program of study or an institution as meeting certain standards (correct)

    • What type of specimen is collected within 30-60 minutes after the dose of a therapeutic drug?

  • Random sample
  • Trough sample
  • Peak sample (correct)
  • Fasting sample

    • Why is it important to collect blood samples in a specific order?

  • To ensure proper labeling of samples
  • To ensure proper mixing of anticoagulants
  • To prevent contamination of blood culture tubes (correct)
  • To prevent clotting

    • What is the term for the evaluation of an individual's competence by a government agency?

    <p>Licensure</p> Signup and view all the answers

    What is the reason for rejecting a specimen that is not collected in a fasting state when required?

    <p>Non-fasting specimen</p> Signup and view all the answers

    What is the term for the evaluation of an individual's competence by a private organization?

    <p>Certification</p> Signup and view all the answers

    What is the relationship between absorbance and concentration according to Beer's Law?

    <p>Absorbance is directly proportional to the concentration</p> Signup and view all the answers

    What is the purpose of the light source in a spectrophotometer?

    <p>To provide a source of light for the reaction</p> Signup and view all the answers

    Which of the following is an exception to the rule that cuvettes must be transparent?

    <p>Reflectance photometry</p> Signup and view all the answers

    What is the role of the monochromator in a spectrophotometer?

    <p>To isolate the specific light of interest</p> Signup and view all the answers

    What is the formula that represents Beer's Law?

    <p>A = abc</p> Signup and view all the answers

    Which of the following light sources cannot provide UV light?

    <p>Tungsten-halogen lamp</p> Signup and view all the answers

    What is the mass in milligrams of 3.5 grams?

    <p>35000</p> Signup and view all the answers

    What percentage of alcohol is needed to make 1L of 70% alcohol if 95% alcohol is used?

    <p>73.7%</p> Signup and view all the answers

    What is the molar mass of anhydrous Na2SO4?

    <p>142</p> Signup and view all the answers

    How much of the hydrated salt Na2SO4·10H2O is needed to replace 8.0g of the anhydrous salt?

    <p>18.14g</p> Signup and view all the answers

    What is the dilution ratio if 3mL of sample is mixed with 12mL of diluent?

    <p>1:5</p> Signup and view all the answers

    What is the anion gap if the sodium level is 140, chloride level is 87, and bicarbonate level is 35?

    <p>13</p> Signup and view all the answers

    What is the purpose of the photodetector in a spectrophotometer?

    <p>To convert transmitted light energy into electrical energy</p> Signup and view all the answers

    What type of cuvettes are used in reflectance photometry?

    <p>Opaque cuvettes</p> Signup and view all the answers

    What is the order of draw for venipuncture and vacutainer tube?

    <p>Citrate Heparin EDTA Fluoride</p> Signup and view all the answers

    What is the purpose of wiping/cleaning the exterior portion of the cuvette?

    <p>To prevent the light from being bounced off</p> Signup and view all the answers

    Why must LDH specimens be stored at room temperature?

    <p>It is a cold-labile enzyme</p> Signup and view all the answers

    What is the unit of measurement produced by the readout device?

    <p>Percent transmittance</p> Signup and view all the answers

    What is the median in a set of numbers?

    <p>The middle value when arranged in order</p> Signup and view all the answers

    Why is it impossible for the spectrophotometer to measure the light attached to the analyte?

    <p>Because the light is absorbed by the analyte</p> Signup and view all the answers

    What is the relationship between percent transmittance and absorbance?

    <p>Percent transmittance is inversely proportional to absorbance</p> Signup and view all the answers

    What is the purpose of measures of central tendency?

    <p>To reflect the accuracy of the data</p> Signup and view all the answers

    What is the function of standard deviation?

    <p>To reflect the variation of data values around the mean</p> Signup and view all the answers

    What is the coefficient of variation?

    <p>A relative indicator of precision</p> Signup and view all the answers

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