8 Questions
What is the primary function of a spectrophotometer?
To measure the concentration of solutes in solution by measuring the amount of light absorbed
What is the principle behind spectrophotometry?
Each compound absorbs or transmits light over a certain range of wavelength
What is the function of a monochromator in a spectrophotometer?
To split light into several component wavelengths
What is the purpose of a wavelength selector in a spectrophotometer?
To transmit only the desired wavelengths
What is the term for the method of measuring how much a chemical substance absorbs light?
Spectrophotometry
What is the application of spectrophotometers in?
Chemistry, physics, biochemistry, material and chemical engineering, and clinical applications
What is the type of spectrophotometer that measures light in the visible range?
Visible spectrophotometer
What is the purpose of plotting a standard curve in spectrophotometry?
To determine the unknown concentration of a sample
Study Notes
What is a Spectrophotometer?
- A spectrophotometer measures the amount of light absorbed by a sample.
- It is used to measure the concentration of solutes in solution by measuring the amount of light absorbed by the solution in a cuvette.
Spectrophotometry
- Spectrophotometry is a method to measure how much a chemical substance absorbs light by measuring the intensity of light as a beam of light passes through a sample solution.
- Each compound absorbs or transmits light over a certain range of wavelength.
- This measurement can also be used to measure the amount of a known chemical substance.
Spectrometer
- A spectrometer produces a desired range of wavelength of light.
- It consists of:
- A collimator (lens) that transmits a straight beam of light (photons).
- A monochromator (prism) that splits the light into several component wavelengths (spectrum).
- A wavelength selector (slit) that transmits only the desired wavelengths.
Applications of Spectrophotometers
- Spectrophotometry is one of the most useful methods of quantitative analysis in various fields, including:
- Chemistry
- Physics
- Biochemistry
- Material and chemical engineering
- Clinical applications
Types of Spectrophotometers
- Visible spectrophotometers
- UV/Visible spectrophotometers
- NIR (Near-Infrared) spectrophotometers
- NMR (Nuclear Magnetic Resonance) spectrophotometers
- Atomic Absorption Spectrophotometers
Using a Spectrophotometer in Lab
- A spectrophotometer is used in lab to measure the concentration of solutes in solution.
- A standard curve is plotted to determine the unknown concentration of a sample.
- A standard curve is a plot of absorbance vs. concentration of a known substance.
Learn about spectrophotometry, an analytical technique used to measure the concentration of solutes in solution. Understand how a spectrophotometer works and its applications.
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