Specimen Collection, Transport and Storage in Medical Microbiology

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24 Questions

What is the recommended storage condition for throat swab specimens?

2-8°C

What is the purpose of the transport of CSF specimens?

Isolation and serology

What is the recommended handling and transportation procedure for water samples if testing is delayed?

Pack the sample on ice

What type of specimen requires storage at -20°C?

NPA/swab in VTM

How should water for bacteriology be handled after collection from a water course or reservoir?

Allow to flow for at least one minute before collection

What is the recommended storage condition for whole blood specimens?

-20°C

What should be added to chlorinated water before collection as a part of the preparation for bacteriology testing?

$0.5$ml of 10% sodium thiosulphate solution

What is the recommended volume for collecting water samples from the source?

At least 200 ml

What is the purpose of quality assurance in clinical diagnostic laboratories?

To maintain the quality of specimens submitted to the laboratory

What determines the clinical utility of the results generated by a Clinical Microbiology laboratory?

The quality of the specimens submitted

Which of the following is part of the pre-analytical process in clinical diagnostic laboratories?

Specimen collection and handling

What type of laboratories require investigations in non-clinical research laboratories?

Non-clinical research laboratories

Which document specifies step-by-step actions needed for each process in a clinical diagnostic laboratory?

Standard operating procedure

What role does transportation play in specimen collection for the Clinical Microbiology laboratory?

It impacts the quality of the specimen submitted to the laboratory

What are the tests conducted on samples in the laboratory called?

Analytical process

What is the primary concern if specimens of poor quality are submitted to a Clinical Microbiology laboratory?

Little or no clinical utility of generated results

Why is it important not to refrigerate blood smears?

Refrigeration alters the cell morphology

What is the appropriate amount of blood to collect for cultures from adults?

5 – 10 ml

What should be done if bacterial endocarditis is suspected when collecting blood for cultures?

Collect three sets of blood culture

What is the purpose of using proper anaerobic collection containers?

To facilitate the survival of organisms

Why is it important to use non-nutritive transport medium for bacteria and viruses?

To prevent organisms from surviving

What should be done if specimens are grossly contaminated or compromised?

They should be rejected

How should blood smears be handled and transported?

They should not be refrigerated

What is the recommended time frame for transporting blood samples for cultures if the laboratory cannot be reached within 24 hours?

Store at 4oC until transportation is possible

Learn about the laboratory investigations carried out in clinical diagnostic laboratories, public health clinical research laboratories, and non-clinical research laboratories. Understand the process, pre-analytical, analytical, and post-analytical stages involved in ensuring quality assurance in clinical diagnostic laboratories.

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