Specimen Collection for Microbiological Studies
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Questions and Answers

Match the specimen type with its collection method:

Body Fluids = Collect using a sterile needle and syringe Throat Swab = Use a cotton swab after good visualization Middle Ear Fluid = Collected by tympanocentesis Corneal Scrapings = Inoculate directly onto bacterial agar media

Match the specimen type with its required transport conditions:

Bronchial Wash = Transport immediately at ambient temperature External Ear Cultures = Processed as superficial wounds Purulent Material from Eye = Transport in appropriate media at ambient temperature Intraocular Fluid = Transport cultures at ambient temperature

Match the specimen type with its minimum volume for collection:

Body Fluids (aerobic/anaerobic) = 10 ml in a sterile container Fungal Hair Specimen = Placed in a dry sterile container Nail Specimen = Scrape to collect the outermost layer Corneal Infections = Multiple scrapings collected for culture

Match the microbiological study with its specific collection hint:

<p>Throat Swab = Reach behind the uvula Nail Specimen = Cleanse with 70-95% ALC Hair Specimen = Scrape the scalp with a blunt scalpel Eye Conjunctiva = Swab as described for purulent material</p> Signup and view all the answers

Match the type of specimen with its acceptable alternatives:

<p>Hair = Hair stubs and contents of plugged follicles Skin Scales = Hair plucked from the scalp with forceps Middle Ear Fluid = Diagnosis indicated by tympanocentesis Corneal Scrapings = Use bacterial agar media or Saboroud’s dextrose agar</p> Signup and view all the answers

Match the circumstance with the specific specimen collection recommendation:

<p>Suspected Tuberculosis = Collect a minimum of 10 ml fluid Otitis Media = Indicate specific ear source Fungal Infection = Collect with a blunt scalpel Purulent Conjunctivitis = Collect purulent material with a cotton swab</p> Signup and view all the answers

Match the specimen and the required technique:

<p>Ear Specimens = Indicate specific ear source during collection Bronchial Lavage = Transport immediately in sterile container Culture media for Corneal Infections = Obtain from microbiology laboratory Skin Scales = Acceptable for MYCOLOGY specimens</p> Signup and view all the answers

Match the specimen collection technique with the collector's experience level:

<p>Body Fluid Collection = Performed by experienced physician Tympanocentesis = Performed by experienced physician Intraocular Fluid Collection = Collected by experienced eye surgeon Bronchial Brush Technique = Performed by experienced chest physician</p> Signup and view all the answers

Study Notes

Specimen Collection for Microbiological Studies

  • Body Fluids (excluding urine and cerebrospinal fluid):
    • Prepare skin as for blood cultures.
    • Collect fluid using a sterile needle and syringe.
    • Submit 10 mL of fluid in a sterile container for aerobic and anaerobic organisms.
    • For suspected tuberculosis or fungal infections, collect a minimum of 10 mL into a sterile container.
    • Transport immediately.
    • Do not send sterile body fluids on swabs.

Bronchial Brush/Wash/Lavage

  • Performed by an experienced chest physician.
  • Transport in a sterile container at ambient temperature.

Throat

  • Use a cotton swab.
  • Use a tongue depressor and good light source for visualization.
  • Swab both tonsillar fauces, posterior pharynx, and any ulceration, exudates, lesions, or inflammation areas.
  • Place swab into appropriate transport media and transport at ambient temperature.

Ear

  • External ear cultures are processed as superficial wounds.
  • Middle ear fluid is processed as a miscellaneous body fluid.
  • For otitis media, the specimen of choice is middle ear fluid collected by tympanocentesis (by an experienced physician).
  • Indicate the specific ear source.

Eye

  • Cleanse skin around the eye with a mild antiseptic.
  • For purulent conjunctivitis, collect purulent material with a cotton swab.
  • Place swabs in appropriate transport media and transport at ambient temperature.

Corneal Infections

  • Obtain culture media from the microbiology laboratory.
  • Swab the conjunctiva as described above.
  • Collect multiple corneal scrapings and inoculate directly onto bacterial agar media (chocolate agar, sheep blood agar, BHI broth) and/or Saboroud's dextrose agar media.
  • Transport at ambient temperature.

Intraocular Fluid

  • Collect fluid by surgical needle aspiration (performed by an experienced eye surgeon).
  • Transport cultures at ambient temperature.

Mycology Specimens (Fungal Only) - Hair

  • Scrap the scalp with a blunt scalpel.
  • Place specimen in a dry, sterile container.
  • Transport at ambient temperature.
  • Hair stubs, contents of plugged follicles, skin scales, and hair plucked with forceps are acceptable.

Mycology Specimens (Fungal Only) - Nails

  • Cleanse the nail with 70-95% ALC.
  • Remove the outermost layer by scraping with a scalpel.
  • Place specimen in a dry, sterile container.
  • Transport at ambient temperature.
  • Acceptable specimens include clippings from discolored/brittle parts of the nail, and deeper scrapings/debris beneath the nail edges.

Mycology Specimens (Fungal Only) - Skin

  • Cleanse the skin with 70-95% ALC.
  • Collect epidermal scales with a scalpel, at the active border of the lesion.
  • Place specimen in a dry, sterile container.
  • Do not tape the specimen to a slide.
  • Transport at ambient temperature.

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Description

This quiz covers essential procedures for collecting specimens for microbiological studies, focusing on various body fluids, bronchial procedures, and throat and ear swabbing techniques. It emphasizes the importance of using sterile equipment and proper transport methods to ensure accurate laboratory results. Ideal for medical students and healthcare professionals.

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