Solution Concentration Dilution and Spectrophotometer Analysis Quiz

The main purpose of this experiment is to prepare and dilute the standard solution of Potassium Permanganate (KMnO4), determine the concentration of the Potassium Permanganate solution, draw the curve of Potassium Permanganate by result of DO and concentrate, determine the concentration of unknown solution by curve, and learn how to use a spectrophotometry machine.

The stock solution concentration of KMnO4 used in this experiment is 2mM.

The stock solution of 2mM KMnO4 is pipetted into six separate 50 mL volumetric flasks, each with varying dilutions (1/20, 1/10, 1/5, 2/5, 1/2, 3/5, 4/5). Then, the solutions in the volumetric flasks are further diluted 10 more times.

<p>The solutions are introduced into cuvettes, the spectrophotometer wavelength is set to 530 nm, and the absorbance is adjusted to zero using a cuvette filled with distilled water as a reference. The DO of each diluted solution is then measured using the spectrophotometer.</p> Signup and view all the answers

<p>By drawing the curve of Potassium Permanganate based on the results of DO and concentration, the concentration of an unknown solution can be determined by comparing its optical density to the curve.</p> Signup and view all the answers

<p>By adding known amounts of analyte to different samples, the concentration of unknown solution can be determined.</p> Signup and view all the answers

<p>The internal standard method involves adding a known amount of a substance different from the analyte to the sample to improve accuracy of measurements.</p> Signup and view all the answers

<p>By plotting the absorbance values of known standard solutions against their concentrations, a linear curve is obtained to determine the analyte concentration in unknown samples.</p> Signup and view all the answers

<p>The curve helps establish a relationship between the concentration of Potassium Permanganate and the optical density, aiding in quantifying unknown concentrations.</p> Signup and view all the answers

<p>By measuring the absorbance of solutions at a specific wavelength, the spectrophotometer provides data to calculate the unknown concentrations.</p> Signup and view all the answers

<p>Diluting the stock solution allows for the preparation of standard solutions with known concentrations for calibration purposes.</p> Signup and view all the answers

<p>Adjusting the absorbance to zero corrects for any background absorbance, ensuring accurate measurements of the samples.</p> Signup and view all the answers

<p>The cuvette serves as a reference to calibrate the spectrophotometer and zero the absorbance before measuring the samples.</p> Signup and view all the answers

<p>Pipettes and volumetric flasks provide precise measurements and allow for the creation of solutions with known concentrations essential for calibration.</p> Signup and view all the answers

<p>Distilled water is added to achieve the desired volume in the volumetric flasks, ensuring accurate dilutions and maintaining the concentration levels.</p> Signup and view all the answers