SNP Chip Technology

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Questions and Answers

Which of the following is NOT a component of a nucleotide?

Hydrogen bond (correct)
Phosphate group
Sugar
Base

What is the purpose of PCR in diagnostic applications?

To detect DNA mutations
To create DNA probes
To amplify DNA (correct)
To sequence DNA

What is the order of the bases in the given DNA sequence?

G T G A C T (correct)
T G T A C A
C T A G T G
A C G T G A

What is a gene?

A specific sequence of DNA (C) Signup and view all the answers

Which base always binds with T in DNA?

A (C) Signup and view all the answers

What is a probe in DNA diagnostics?

A few nucleotides of DNA (B) Signup and view all the answers

What is the purpose of a probe in DNA diagnostics?

To identify specific sequences of DNA (C) Signup and view all the answers

What is the most sensitive means of diagnosing a viral infection or detecting contamination?

DNA sequencing (C) Signup and view all the answers

Which enzyme is used in the PCR reaction to amplify DNA?

Taq polymerase (D) Signup and view all the answers

What is the purpose of qPCR?

To calculate the amount of a specific gene or RNA present (C) Signup and view all the answers

What is the function of the thermocycler in PCR?

To heat up and cool down the DNA sample (A) Signup and view all the answers

What does the straight line on the qPCR graph indicate?

Increasing concentration of DNA or RNA (B) Signup and view all the answers

Which technology allows for data on the sequence of many genes at once?

SNP chips (B) Signup and view all the answers

Which company makes the HiSeq?

Illumina (A) Signup and view all the answers

What is the base pair range for the HiSeq and ion proton?

150-200 base pairs (A) Signup and view all the answers

How much does it cost to sequence one human being's genome currently?

$1000 (C) Signup and view all the answers

Which enzyme is responsible for copying a single strand of RNA into a piece of DNA?

Reverse transcriptase (D) Signup and view all the answers

What is the purpose of copying RNA into DNA using reverse transcriptase?

To prevent degradation of the RNA sample (A) Signup and view all the answers

What does cDNA stand for?

Copied DNA (C) Signup and view all the answers

What is the function of an SNP Chip in detecting single nucleotide polymorphisms?

To identify single base changes in DNA sequences (C) Signup and view all the answers

Which of the following is true about SNP chips?

SNP chips are used to detect single nucleotide polymorphisms (SNPs) in DNA sequences. (A) Signup and view all the answers

What is the purpose of attaching a fluorescent label to a patient's DNA in an SNP chip?

To detect sequence differences in the patient's DNA. (A) Signup and view all the answers

What does it mean if a patient's DNA binds in the first grid of an SNP chip?

The patient has a low risk of developing Alzheimer's disease. (A) Signup and view all the answers

What is the purpose of comparing the DNA sequences of normal patients to those with the disease?

To detect sequence differences in the DNA sequences. (C) Signup and view all the answers

Which nucleotide is complimentary to the first base on the single-stranded DNA, which is a T?

A (D) Signup and view all the answers

What prevents any additional nucleotides from being added on to the free nucleotide?

The fluorescent dye (A) Signup and view all the answers

What happens to the nucleotide after the termination chemical is removed?

It becomes a normal nucleotide (B) Signup and view all the answers

What is the purpose of adding a chemical that removes the terminator?

To block the incorporation of the next nucleotide (A) Signup and view all the answers

What happens to the nucleotide after the dye is removed?

It becomes colorless (B) Signup and view all the answers

What is the purpose of the termination chemical on the A nucleotide?

To block the incorporation of the next nucleotide (C) Signup and view all the answers

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