How Diagnostic Tests Work: DNA-Based Diagnostics
30 Questions
1 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

Which enzyme is responsible for copying RNA into DNA?

  • Taq polymerase
  • Reverse transcriptase (correct)
  • Express SYBER Green
  • Polymerase

    • What is the purpose of copying RNA into DNA?

  • To prevent RNA degradation (correct)
  • To amplify RNA
  • To visualize RNA
  • To stabilize RNA

    • What does cDNA stand for?

  • Chemically stable DNA
  • Copied DNA
  • Complementary DNA (correct)
  • Conserved DNA

    • What is the purpose of using an SNP Chip in diagnostics?

    <p>To detect single nucleotide polymorphisms</p> Signup and view all the answers

    Which component is required for Polymerase Chain Reaction (PCR)?

    <p>Thermocycler</p> Signup and view all the answers

    What is the purpose of the DNA primer in PCR?

    <p>To specify the DNA sequence</p> Signup and view all the answers

    What is the purpose of Taq polymerase in PCR?

    <p>To copy the DNA</p> Signup and view all the answers

    What is the purpose of qPCR (quantitative PCR)?

    <p>To calculate the amount of a specific gene or RNA present</p> Signup and view all the answers

    Which of the following is NOT a component of a nucleotide?

    <p>Hydrogen bond</p> Signup and view all the answers

    What does DNA stand for?

    <p>Deoxyribonucleic acid</p> Signup and view all the answers

    What is the order of the bases in the given DNA sequence: G T G A C T?

    <p>Guanine, Adenosine, Guanine, Cytosine, Thymine, Adenosine</p> Signup and view all the answers

    Which company makes the HiSeq sequencing instrument?

    <p>Illumina</p> Signup and view all the answers

    What is a gene?

    <p>A specific sequence of DNA</p> Signup and view all the answers

    What is the base pair range for the HiSeq and Ion Proton sequencing instruments?

    <p>150-200 base pairs</p> Signup and view all the answers

    What is the advantage of SNP chips over microarray chips?

    <p>SNP chips can detect hundreds of thousands of DNA sequences simultaneously</p> Signup and view all the answers

    What is the purpose of whole genome sequencing?

    <p>To diagnose genetic differences between normal physiology and a disease state</p> Signup and view all the answers

    Which of the following is true about complementary base pairing in DNA?

    <p>A binds with C and T binds with G</p> Signup and view all the answers

    What is a DNA probe?

    <p>A short sequence of chemically produced DNA</p> Signup and view all the answers

    What is the purpose of using a DNA probe in diagnostic DNA testing?

    <p>To locate and identify specific sequences of DNA</p> Signup and view all the answers

    Which technique is commonly used for the detection of specific DNA sequences?

    <p>Polymerase chain reaction (PCR)</p> Signup and view all the answers

    Which of the following is the purpose of using a SNP chip in DNA assays?

    <p>To detect sequence differences in DNA</p> Signup and view all the answers

    What is the function of a CCD camera in SNP chip analysis?

    <p>To capture the output colors from the chip</p> Signup and view all the answers

    What does it mean if a patient's DNA binds in the first grid of an SNP chip?

    <p>The patient does not have the e4 allele or gene</p> Signup and view all the answers

    What is the purpose of attaching a fluorescent label to the patient's DNA in SNP chip analysis?

    <p>To visualize the binding of the patient's DNA to the chip</p> Signup and view all the answers

    Which nucleotide is complementary to the first base, T, on the single-stranded DNA?

    <p>A</p> Signup and view all the answers

    What prevents the addition of additional nucleotides after the incorporation of the first A?

    <p>Termination chemical</p> Signup and view all the answers

    What happens to the color of the nucleotide after the dye is removed?

    <p>It becomes colorless</p> Signup and view all the answers

    What is the purpose of the termination chemical during the sequencing process?

    <p>To prevent the addition of nucleotides</p> Signup and view all the answers

    What is the first nucleotide incorporated into the short probe chain after the T base?

    <p>A</p> Signup and view all the answers

    What happens to the nucleotide after the termination chemical is removed?

    <p>It becomes darker</p> Signup and view all the answers

    More Like This

    Use Quizgecko on...
    Browser
    Open
    Browser
    Browser