30 Questions
Which enzyme is responsible for copying RNA into DNA?
Reverse transcriptase
What is the purpose of copying RNA into DNA?
To prevent RNA degradation
What does cDNA stand for?
Complementary DNA
What is the purpose of using an SNP Chip in diagnostics?
To detect single nucleotide polymorphisms
Which component is required for Polymerase Chain Reaction (PCR)?
Thermocycler
What is the purpose of the DNA primer in PCR?
To specify the DNA sequence
What is the purpose of Taq polymerase in PCR?
To copy the DNA
What is the purpose of qPCR (quantitative PCR)?
To calculate the amount of a specific gene or RNA present
Which of the following is NOT a component of a nucleotide?
Hydrogen bond
What does DNA stand for?
Deoxyribonucleic acid
What is the order of the bases in the given DNA sequence: G T G A C T?
Guanine, Adenosine, Guanine, Cytosine, Thymine, Adenosine
Which company makes the HiSeq sequencing instrument?
Illumina
What is a gene?
A specific sequence of DNA
What is the base pair range for the HiSeq and Ion Proton sequencing instruments?
150-200 base pairs
What is the advantage of SNP chips over microarray chips?
SNP chips can detect hundreds of thousands of DNA sequences simultaneously
What is the purpose of whole genome sequencing?
To diagnose genetic differences between normal physiology and a disease state
Which of the following is true about complementary base pairing in DNA?
A binds with C and T binds with G
What is a DNA probe?
A short sequence of chemically produced DNA
What is the purpose of using a DNA probe in diagnostic DNA testing?
To locate and identify specific sequences of DNA
Which technique is commonly used for the detection of specific DNA sequences?
Polymerase chain reaction (PCR)
Which of the following is the purpose of using a SNP chip in DNA assays?
To detect sequence differences in DNA
What is the function of a CCD camera in SNP chip analysis?
To capture the output colors from the chip
What does it mean if a patient's DNA binds in the first grid of an SNP chip?
The patient does not have the e4 allele or gene
What is the purpose of attaching a fluorescent label to the patient's DNA in SNP chip analysis?
To visualize the binding of the patient's DNA to the chip
Which nucleotide is complementary to the first base, T, on the single-stranded DNA?
A
What prevents the addition of additional nucleotides after the incorporation of the first A?
Termination chemical
What happens to the color of the nucleotide after the dye is removed?
It becomes colorless
What is the purpose of the termination chemical during the sequencing process?
To prevent the addition of nucleotides
What is the first nucleotide incorporated into the short probe chain after the T base?
A
What happens to the nucleotide after the termination chemical is removed?
It becomes darker
N/A
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