Biochem Lecture 13
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Questions and Answers

Where does the first steps in the construction of the N-linked oligosaccharide moiety of a glycoprotein occur?

  • Mitochondria
  • Cytosolic face of the ER (correct)
  • Lumen of the Golgi complex
  • Nucleus
  • What blocks the first step of glycosylation by mimicking the structure of UDP-GlcNac?

  • Streptomycin
  • Vancomycin
  • Tunicamycin (correct)
  • Penicillin
  • After the core oligosaccharide is transferred to an Asn residue of the protein within the ER lumen, where does further modification of the core oligosaccharide occur?

  • Cytoplasm
  • ER and the Golgi complex (correct)
  • Cell membrane
  • Endosome
  • What are dolichol phosphate derivatives involved in during glycoprotein synthesis?

    <p>Contributing mannose and glucose residues to the oligosaccharide</p> Signup and view all the answers

    What is the role of the signal recognition particle (SRP) in protein targeting?

    <p>Binds to the signal sequence of the nascent polypeptide</p> Signup and view all the answers

    Which organelle is responsible for sorting proteins for membrane insertion, secretion, or delivery to other organelles?

    <p>Golgi apparatus</p> Signup and view all the answers

    What is the function of chaperone proteins in the targeting of proteins to mitochondria and chloroplasts?

    <p>Assist in protein translocation and folding</p> Signup and view all the answers

    Which site initiates post-translational modification in many eukaryotic proteins?

    <p>Endoplasmic Reticulum</p> Signup and view all the answers

    What type of bond do O-linked oligosaccharides form with the hydroxyl group of Ser or Thr residues?

    <p>Glycosidic bond</p> Signup and view all the answers

    How are N-linked oligosaccharides bonded to the amide nitrogen of an Asn residue?

    <p>N-glycosyl bond</p> Signup and view all the answers

    Which type of oligosaccharide has a requirement for specifying the position and stereochemistry of each glycosidic linkage?

    <p>N-linked oligosaccharides</p> Signup and view all the answers

    What is the main function of the signal sequence in proteins?

    <p>Directing the ribosome to the SRP and the ER</p> Signup and view all the answers

    Which protein subunit of SRP plays a role in obstructing elongation during protein synthesis?

    <p>Protein subunit binding sterically to aminoacyl-tRNAs</p> Signup and view all the answers

    What is the function of the SRP receptor in the protein translocation process?

    <p>Recognizing and interacting with the SRP-ribosome complex</p> Signup and view all the answers

    Which organelle is primarily involved in glycosylation of proteins?

    <p>Endoplasmic reticulum (ER)</p> Signup and view all the answers

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