15 Questions
Where does the first steps in the construction of the N-linked oligosaccharide moiety of a glycoprotein occur?
Cytosolic face of the ER
What blocks the first step of glycosylation by mimicking the structure of UDP-GlcNac?
Tunicamycin
After the core oligosaccharide is transferred to an Asn residue of the protein within the ER lumen, where does further modification of the core oligosaccharide occur?
ER and the Golgi complex
What are dolichol phosphate derivatives involved in during glycoprotein synthesis?
Contributing mannose and glucose residues to the oligosaccharide
What is the role of the signal recognition particle (SRP) in protein targeting?
Binds to the signal sequence of the nascent polypeptide
Which organelle is responsible for sorting proteins for membrane insertion, secretion, or delivery to other organelles?
Golgi apparatus
What is the function of chaperone proteins in the targeting of proteins to mitochondria and chloroplasts?
Assist in protein translocation and folding
Which site initiates post-translational modification in many eukaryotic proteins?
Endoplasmic Reticulum
What type of bond do O-linked oligosaccharides form with the hydroxyl group of Ser or Thr residues?
Glycosidic bond
How are N-linked oligosaccharides bonded to the amide nitrogen of an Asn residue?
N-glycosyl bond
Which type of oligosaccharide has a requirement for specifying the position and stereochemistry of each glycosidic linkage?
N-linked oligosaccharides
What is the main function of the signal sequence in proteins?
Directing the ribosome to the SRP and the ER
Which protein subunit of SRP plays a role in obstructing elongation during protein synthesis?
Protein subunit binding sterically to aminoacyl-tRNAs
What is the function of the SRP receptor in the protein translocation process?
Recognizing and interacting with the SRP-ribosome complex
Which organelle is primarily involved in glycosylation of proteins?
Endoplasmic reticulum (ER)
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