Serum Albumin Binding
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Questions and Answers

How was solid-phase microextraction used to analyze fatty acid binding to serum albumin?

  • By measuring the concentration of bound ligands only
  • By measuring the concentration of free ligands only
  • By measuring the amount of free and bound ligands via desorption and adsorption principles (correct)
  • By using a complex chemical reaction to determine ligand binding
  • Why was the stoichiometric binding approach chosen as the more practical and effective approach in the study?

  • It provides a more accurate measurement of free ligands
  • It allows for individual analysis of each binding site
  • It is more practical in cases where the cooperation among the binding sites is difficult to interpret (correct)
  • It simplifies the process of ligand binding analysis
  • With what atom in the PVP-AgNPs do mercury ions bind to?

  • Silver atoms (correct)
  • Peroxidases
  • O-phenylenediamine
  • 2,3-diaminophenazine
  • What kind of enzymes do the PVP-AgNPs mimic?

    <p>Peroxidases</p> Signup and view all the answers

    What compound corresponds to the strong fluorescence peak observed at 510 nm in the spectral characterization analysis of frozen pork by Zhuang et al., 2023?

    <p>Riboflavin</p> Signup and view all the answers

    In Zhuang et al., 2023, which statement accurately reflects the relationship between TVB-N value and pork freshness?

    <p>The lower the TVB-N value, the fresher the meat.</p> Signup and view all the answers

    In the study probing menstrual bloodstain aging with fluorescence spectroscopy, which compounds were detected?

    <p>Tryptophan and NADH</p> Signup and view all the answers

    Based on the main findings of the paper, what model is followed by the in vivo and in vitro relationships of bound and free insulin?

    <p>in vivo: two-site model, in vitro: one-site model</p> Signup and view all the answers

    To evaluate the Ka and Bmax of the anti-insulin antibodies more accurately in the one-site model, the researchers analyzed the saturation binding curve in vivo by ______.

    <p>non-linear least squares fitting</p> Signup and view all the answers

    At what point in the in vitro binding-site model of insulin is the Scatchard plot similar to the linear expression in vivo?

    <p>As B approaches 0</p> Signup and view all the answers

    According to the paper, what happens when the insulin concentrations exceed the binding capacity of the high affinity site in vivo?

    <p>The B/F vs B plots becomes curvilinear</p> Signup and view all the answers

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