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Questions and Answers
What is serology?
What is serology?
What does ELISA detect?
What does ELISA detect?
What is the purpose of Western Blotting?
What is the purpose of Western Blotting?
What do Immunofluorescence Assays use to detect specific antigens or antibodies?
What do Immunofluorescence Assays use to detect specific antigens or antibodies?
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What does the Complement Fixation Test measure?
What does the Complement Fixation Test measure?
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Study Notes
Serology
- Serology is the study of blood serum and its components, focusing on the immune response to pathogens or foreign substances.
- It is commonly used to detect antibodies in blood, which indicate previous exposure to infections or immunizations.
ELISA
- Enzyme-Linked Immunosorbent Assay (ELISA) is a common laboratory technique that detects and quantifies proteins, peptides, antibodies, and hormones in a sample.
- It is widely used for diagnosing diseases, such as infections and allergies, by measuring specific antibodies in the serum.
Western Blotting
- Western Blotting is a biochemical technique used to detect specific proteins in a sample through gel electrophoresis followed by transfer to a membrane.
- It serves as a confirmatory test for various infections, including HIV, ensuring the presence of specific antibodies against the pathogen.
Immunofluorescence Assays
- Immunofluorescence Assays utilize fluorescently labeled antibodies to detect specific antigens or antibodies within cells or tissue sections.
- This method allows for visualization and localization of proteins, aiding in the diagnosis of autoimmune diseases and infections.
Complement Fixation Test
- The Complement Fixation Test measures the ability of serum antibodies to fix complement proteins in the presence of a specific antigen.
- It is utilized for the diagnosis of certain infections by indicating whether the immune system has reacted to a particular pathogen.
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Description
Test your knowledge of serology with this quiz! Explore the world of blood serum analysis, immune system responses, and the Enzyme-Linked Immunosorbent Assay (ELISA). Sharpen your understanding of this vital branch of science.