Serology Applied to Plant Virology

The study of viruses that infect plants.

Crops, fruits, and vegetables.

Damage to agricultural crops caused by viruses.

Using blood serum to detect pathogens (like viruses).

Methods for identifying plant viruses.

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Effective plant disease control requires precise knowledge of causal agents, dissemination, and survival strategies of the disease, along with a correct laboratory diagnosis.

Identifying plant virus infections can be challenging due to variable symptoms depending on plant variety, environment, virus strain, and the possibility of multiple infections.

Using a series of indicator plants to detect and identify the presence of plant viruses.

Modern methods used for accurate plant virus disease diagnosis, encompassing various aspects of virus biology (physical, biological, cytological, serological, molecular).

Highly specific and relatively simple laboratory methods for rapid and precise plant virus identification.

A technique used to detect viruses in plant tissues, utilizing antibodies against the virus that bind to the virus's antigen.

A molecular biology technique allowing scientists to amplify specific DNA fragments within a sample.

Methods to identify plant viruses, often using immunological or molecular approaches.

A method to detect plant viruses and subviral pathogens in microtiter plates, combining virus capture using antibodies and subsequent PCR amplification.

Protective protein layers that encase plant virus particles, involved in viral recognition and transmission.

Detection of plant viruses using antibody-antigen reactions, for example, immunodiffusion or ELISA.

Small, multiple-well plates used in various scientific experiments.

A technique to detect antibodies and antigens by observing their reaction and diffusion pattern when combined in a gel.

A strain-specific ELISA method that uses antibodies conjugated to an enzyme (like alkaline phosphatase) to detect virus antigens.

A method that avoids enzyme conjugation by using antibodies from two different species and a universal enzyme conjugate to detect virus particles trapped in wells.

Another name for Direct ELISA, emphasizing the sandwich-like structure with antibodies.

An antibody that has an enzyme attached to it; used in ELISA methods.

A common enzyme used to create a color change in ELISA assays.

A technique used to separate substances of different densities by spinning them at high speed.

Enzyme-linked immunosorbent assay; a technique to detect antigens or antibodies using enzymes.

A serological technique detecting antigens and antibodies by their reaction in agar gel.

Serum containing antibodies produced in response to an antigen.

Immunizing an animal (like rabbit or mouse) through oral intake of a substance (virus).

Serological methods where one reagent (antibody) is bound to a solid surface.

Serological methods where antigen and antibody react in a liquid medium, like agar.

Protein on the surface of a virus particle, often used in serological testing.

Methods using serum (blood) to identify viruses.

Variation of a pathogen with similar but not identical characteristics.

A plant virus that infects various crops, including tropical crops.

A plant virus affecting passion fruit plants.

Antibody solution produced by immunizing an animal (e.g., rabbit) with a virus preparation.

The process of injecting rabbits with purified virus preparations to produce antibodies against it.

The method of isolating the virus from other plant components before immunization.

A process of stimulating the immune system of an animal to produce antibodies against a specific target.

A method used to reduce the concentration of antiserum to a suitable level, measured as a ratio, to ensure optimal results during testing.

Different ways to introduce an antigen into a rabbit's body to stimulate an immune response, including intravenous, intramuscular, and foot pad injections.