Podcast
Questions and Answers
What is the primary purpose of De-novo assembly in NGS?
What is the primary purpose of De-novo assembly in NGS?
- To annotate bacterial and archaeal genomes
- To detect SNPs and structural variations (correct)
- To identify the genome size
- To assess the quality of assembled data
What determines the number of times sequenced nucleotide bases cover the target genome?
What determines the number of times sequenced nucleotide bases cover the target genome?
- Genome size
- Sequencing depth (correct)
- Contig length
- Sequencing coverage
What is the definition of N50 score?
What is the definition of N50 score?
- The number of contigs in the assembly
- The sequence length of the shortest contig at 50% of the assembly length (correct)
- The longest contig in the assembly
- The total length of all contigs
What is the purpose of RAST annotation?
What is the purpose of RAST annotation?
What is the minimum sequencing coverage required for De-novo assembly?
What is the minimum sequencing coverage required for De-novo assembly?
What is the purpose of FastQC?
What is the purpose of FastQC?
What is the purpose of sequence assembly?
What is the purpose of sequence assembly?
What is the term for the smallest number of contigs whose length sum makes up half of the assembly size?
What is the term for the smallest number of contigs whose length sum makes up half of the assembly size?
What is a characteristic of re-sequencing assembly?
What is a characteristic of re-sequencing assembly?
What is the relationship between Phred Quality Scores (Q scores) and base calling error probabilities (P)?
What is the relationship between Phred Quality Scores (Q scores) and base calling error probabilities (P)?
What is the advantage of re-sequencing assembly over de novo assembly?
What is the advantage of re-sequencing assembly over de novo assembly?
What is the name of a commonly used tool for sequence assembly?
What is the name of a commonly used tool for sequence assembly?
What was the first fully sequenced amino acid sequence in 1953?
What was the first fully sequenced amino acid sequence in 1953?
What is a limitation of the chain-termination method in DNA sequencing?
What is a limitation of the chain-termination method in DNA sequencing?
What is the purpose of shotgun sequencing?
What is the purpose of shotgun sequencing?
What is the difference between clone-by-clone and whole-genome shotgun sequencing?
What is the difference between clone-by-clone and whole-genome shotgun sequencing?
What is the first step in DNA sequencing?
What is the first step in DNA sequencing?
What is the advantage of whole-genome sequencing?
What is the advantage of whole-genome sequencing?
