Biochemistry: Molisch's Test Principle

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8 Questions

What is the basic principle involved in the Molisch's test for carbohydrates?

Formation of aldehyde by dehydration

Which reagent is used in the Iodine test for detecting carbohydrates?

Iodine and potassium iodide

What color change indicates a positive result in the Molisch's test for carbohydrates?

Purple or violet

Why is potassium iodide used in making the IKI solution for the Iodine test?

To dissociate into iodide ions

In the Iodine test, what type of ions combine to form polyiodide ions?

Iodide ions

What do polyiodide ions in the Iodine test form a complex with?

Amylose

Why does molecular iodine need potassium iodide to form the IKI solution?

To make it soluble in water

What happens when an aldehyde formed in Molisch's test condenses with two phenol-type molecules?

Formation of a purple or violet ring

Learn about the principle behind Molisch's test, where carbohydrates undergo dehydration in the presence of concentrated sulphuric acid to form a purple or violet colored ring. Understand the procedure and interpretation of results.

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