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Questions and Answers
What is the basic principle involved in the Molisch's test for carbohydrates?
What is the basic principle involved in the Molisch's test for carbohydrates?
Which reagent is used in the Iodine test for detecting carbohydrates?
Which reagent is used in the Iodine test for detecting carbohydrates?
What color change indicates a positive result in the Molisch's test for carbohydrates?
What color change indicates a positive result in the Molisch's test for carbohydrates?
Why is potassium iodide used in making the IKI solution for the Iodine test?
Why is potassium iodide used in making the IKI solution for the Iodine test?
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In the Iodine test, what type of ions combine to form polyiodide ions?
In the Iodine test, what type of ions combine to form polyiodide ions?
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What do polyiodide ions in the Iodine test form a complex with?
What do polyiodide ions in the Iodine test form a complex with?
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Why does molecular iodine need potassium iodide to form the IKI solution?
Why does molecular iodine need potassium iodide to form the IKI solution?
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What happens when an aldehyde formed in Molisch's test condenses with two phenol-type molecules?
What happens when an aldehyde formed in Molisch's test condenses with two phenol-type molecules?
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Study Notes
Molisch's Test for Carbohydrates
- The basic principle involved in Molisch's test is that aldehydes are formed when carbohydrates are dehydrated in the presence of sulfuric acid.
- The test is used to detect the presence of carbohydrates.
Iodine Test for Carbohydrates
- α-Iodine (IKI) solution is used as the reagent in the Iodine test for detecting carbohydrates.
- A blue or purple color change indicates a positive result in the Iodine test, indicating the presence of carbohydrates.
IKI Solution
- Potassium iodide (KI) is used in making the IKI solution because it stabilizes the iodine and allows it to form polyiodide ions.
- In the Iodine test, iodide ions (I-) combine with iodine molecules (I2) to form polyiodide ions.
- Polyiodide ions in the Iodine test form a complex with starch, which is a type of carbohydrate.
- Molecular iodine (I2) needs potassium iodide (KI) to form the IKI solution because it is not soluble in water.
Molisch's Test Reaction
- When an aldehyde formed in Molisch's test condenses with two phenol-type molecules, it forms a colored compound, indicating a positive result for the presence of carbohydrates.
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Description
Learn about the principle behind Molisch's test, where carbohydrates undergo dehydration in the presence of concentrated sulphuric acid to form a purple or violet colored ring. Understand the procedure and interpretation of results.
