micro 7
30 Questions
1 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the primary mechanism by which MacConkey Agar suppresses the growth of Gram-positive organisms?

  • Presence of bile salts (correct)
  • Neutral red dye
  • High glucose concentration
  • Crystal violet

    • Which type of ELISA allows for the capture of specific antigens?

  • Indirect ELISA
  • Direct ELISA
  • Sandwich ELISA (correct)
  • Competitive ELISA

    • Which of the following best describes the function of neutral red dye in MacConkey Agar?

  • Inhibits the growth of Gram-negative organisms
  • Forms black colonies in the presence of tellurite
  • Promotes the growth of Staphylococci
  • Turns pink in acid conditions (correct)

    • In molecular identification using Genomic PCR, what determines the sequences that are amplified?

    <p>Primers</p> Signup and view all the answers

    What makes Sabouraud Dextrose Agar selective for certain fungi?

    <p>Low pH (5.6)</p> Signup and view all the answers

    Which component of Baird-Parker Agar promotes the growth of Staphylococci?

    <p>Pyruvate</p> Signup and view all the answers

    What type of identification method is used to amplify housekeeping genes and sequence 7 specific genes?

    <p>Multi locus sequence typing (MLST)</p> Signup and view all the answers

    What is the main purpose of Genomic DNA Sequencing?

    <p>To sequence whole genomes or targeted regions</p> Signup and view all the answers

    What is the main function of Mannitol Salt Agar in differentiating between bacterial species?

    <p>Presence of phenol red</p> Signup and view all the answers

    How does Eosin Methylene Blue (EMB) Agar differentiate between Gram-negative and Gram-positive organisms?

    <p>Presence of eosin and methylene blue stains</p> Signup and view all the answers

    Which technique is specifically used for the identification of antibiotic resistance like mecA?

    <p>Genomic PCR</p> Signup and view all the answers

    What type of antibodies are required for the Direct ELISA method?

    <p>Monoclonal antibodies</p> Signup and view all the answers

    Which staining method is commonly used for endospore staining?

    <p>Malachite Green Stain</p> Signup and view all the answers

    What type of bacteria are mainly targeted by Ziehl–Neelsen stain?

    <p>Acid-fast bacteria</p> Signup and view all the answers

    Which staining method is commonly used to detect polysaccharides in fungi?

    <p>Periodic acid-Schiff stain</p> Signup and view all the answers

    In which type of microscopy is Gram Staining the first step in identifying pure bacterial cultures?

    <p>Transmission electron microscopy (TEM)</p> Signup and view all the answers

    Which biochemical test can differentiate between bacteria that belong to Pseudomonadaceae and Enterobacteriaceae?

    <p>Oxidase test</p> Signup and view all the answers

    Which technique is commonly used for the definitive identification of bacterial species?

    <p>Whole Genome Sequencing</p> Signup and view all the answers

    What method is increasingly used for identifying microbial contaminants due to its speed and lower cost compared to conventional methods?

    <p>MALDI TOF</p> Signup and view all the answers

    Which agar can be used to identify bacterial species from selective media?

    <p>MacConkey Agar</p> Signup and view all the answers

    What is the need for balancing between turnaround time and sensitivity in microbial identification techniques?

    <p>To optimize efficiency</p> Signup and view all the answers

    Which method can provide information about resistance in microbial samples?

    <p>MALDI TOF</p> Signup and view all the answers

    In microbial identification, which of the following databases exist for different bacteria species?

    <p><a href="https://pubmlst.org/">https://pubmlst.org/</a></p> Signup and view all the answers

    Which chapter of 'Hugo and Russell’s Pharmaceutical Microbiology' discusses the ideal diagnostic characteristics including accuracy, sensitivity, cost-effectiveness, and speed?

    <p>Chapter 3 Section 8</p> Signup and view all the answers

    What is the main purpose of using selective media such as Mannitol Salt Agar in microbial identification?

    <p>To inhibit the growth of unwanted bacteria while allowing the growth of target bacteria</p> Signup and view all the answers

    In microbial identification, what is the primary purpose of using MacConkey Agar?

    <p>To differentiate between Gram-positive and Gram-negative bacteria based on lactose fermentation</p> Signup and view all the answers

    What is the significance of Baird-Parker Agar in microbiology?

    <p>It is employed in the isolation and enumeration of Staphylococci, particularly Staphylococcus aureus</p> Signup and view all the answers

    Sabouraud Dextrose Agar is primarily used for which type of microorganism identification?

    <p>Yeast and molds</p> Signup and view all the answers

    Which statement best describes the role of differential media in microbial identification?

    <p>Differential media help in distinguishing between different species or types of microorganisms based on specific biochemical reactions.</p> Signup and view all the answers

    How do selective and differential media complement each other in microbial identification?

    <p>Selective media inhibit unwanted bacteria, while differential media help differentiate between species based on colony morphology.</p> Signup and view all the answers

    More Like This

    Use Quizgecko on...
    Browser
    Open
    Browser
    Browser