micro 7

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Questions and Answers

What is the primary mechanism by which MacConkey Agar suppresses the growth of Gram-positive organisms?

  • Presence of bile salts (correct)
  • Neutral red dye
  • High glucose concentration
  • Crystal violet

Which type of ELISA allows for the capture of specific antigens?

  • Indirect ELISA
  • Direct ELISA
  • Sandwich ELISA (correct)
  • Competitive ELISA

Which of the following best describes the function of neutral red dye in MacConkey Agar?

  • Inhibits the growth of Gram-negative organisms
  • Forms black colonies in the presence of tellurite
  • Promotes the growth of Staphylococci
  • Turns pink in acid conditions (correct)

In molecular identification using Genomic PCR, what determines the sequences that are amplified?

<p>Primers (A)</p> Signup and view all the answers

What makes Sabouraud Dextrose Agar selective for certain fungi?

<p>Low pH (5.6) (D)</p> Signup and view all the answers

Which component of Baird-Parker Agar promotes the growth of Staphylococci?

<p>Pyruvate (D)</p> Signup and view all the answers

What type of identification method is used to amplify housekeeping genes and sequence 7 specific genes?

<p>Multi locus sequence typing (MLST) (D)</p> Signup and view all the answers

What is the main purpose of Genomic DNA Sequencing?

<p>To sequence whole genomes or targeted regions (D)</p> Signup and view all the answers

What is the main function of Mannitol Salt Agar in differentiating between bacterial species?

<p>Presence of phenol red (B)</p> Signup and view all the answers

How does Eosin Methylene Blue (EMB) Agar differentiate between Gram-negative and Gram-positive organisms?

<p>Presence of eosin and methylene blue stains (D)</p> Signup and view all the answers

Which technique is specifically used for the identification of antibiotic resistance like mecA?

<p>Genomic PCR (A)</p> Signup and view all the answers

What type of antibodies are required for the Direct ELISA method?

<p>Monoclonal antibodies (B)</p> Signup and view all the answers

Which staining method is commonly used for endospore staining?

<p>Malachite Green Stain (D)</p> Signup and view all the answers

What type of bacteria are mainly targeted by Ziehl–Neelsen stain?

<p>Acid-fast bacteria (A)</p> Signup and view all the answers

Which staining method is commonly used to detect polysaccharides in fungi?

<p>Periodic acid-Schiff stain (A)</p> Signup and view all the answers

In which type of microscopy is Gram Staining the first step in identifying pure bacterial cultures?

<p>Transmission electron microscopy (TEM) (A)</p> Signup and view all the answers

Which biochemical test can differentiate between bacteria that belong to Pseudomonadaceae and Enterobacteriaceae?

<p>Oxidase test (B)</p> Signup and view all the answers

Which technique is commonly used for the definitive identification of bacterial species?

<p>Whole Genome Sequencing (C)</p> Signup and view all the answers

What method is increasingly used for identifying microbial contaminants due to its speed and lower cost compared to conventional methods?

<p>MALDI TOF (D)</p> Signup and view all the answers

Which agar can be used to identify bacterial species from selective media?

<p>MacConkey Agar (C)</p> Signup and view all the answers

What is the need for balancing between turnaround time and sensitivity in microbial identification techniques?

<p>To optimize efficiency (B)</p> Signup and view all the answers

Which method can provide information about resistance in microbial samples?

<p>MALDI TOF (D)</p> Signup and view all the answers

In microbial identification, which of the following databases exist for different bacteria species?

<p><a href="https://pubmlst.org/">https://pubmlst.org/</a> (C)</p> Signup and view all the answers

Which chapter of 'Hugo and Russell’s Pharmaceutical Microbiology' discusses the ideal diagnostic characteristics including accuracy, sensitivity, cost-effectiveness, and speed?

<p>Chapter 3 Section 8 (A)</p> Signup and view all the answers

What is the main purpose of using selective media such as Mannitol Salt Agar in microbial identification?

<p>To inhibit the growth of unwanted bacteria while allowing the growth of target bacteria (A)</p> Signup and view all the answers

In microbial identification, what is the primary purpose of using MacConkey Agar?

<p>To differentiate between Gram-positive and Gram-negative bacteria based on lactose fermentation (A)</p> Signup and view all the answers

What is the significance of Baird-Parker Agar in microbiology?

<p>It is employed in the isolation and enumeration of Staphylococci, particularly Staphylococcus aureus (B)</p> Signup and view all the answers

Sabouraud Dextrose Agar is primarily used for which type of microorganism identification?

<p>Yeast and molds (A)</p> Signup and view all the answers

Which statement best describes the role of differential media in microbial identification?

<p>Differential media help in distinguishing between different species or types of microorganisms based on specific biochemical reactions. (C)</p> Signup and view all the answers

How do selective and differential media complement each other in microbial identification?

<p>Selective media inhibit unwanted bacteria, while differential media help differentiate between species based on colony morphology. (A)</p> Signup and view all the answers

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