30 Questions
What is the primary mechanism by which MacConkey Agar suppresses the growth of Gram-positive organisms?
Presence of bile salts
Which type of ELISA allows for the capture of specific antigens?
Sandwich ELISA
Which of the following best describes the function of neutral red dye in MacConkey Agar?
Turns pink in acid conditions
In molecular identification using Genomic PCR, what determines the sequences that are amplified?
Primers
What makes Sabouraud Dextrose Agar selective for certain fungi?
Low pH (5.6)
Which component of Baird-Parker Agar promotes the growth of Staphylococci?
Pyruvate
What type of identification method is used to amplify housekeeping genes and sequence 7 specific genes?
Multi locus sequence typing (MLST)
What is the main purpose of Genomic DNA Sequencing?
To sequence whole genomes or targeted regions
What is the main function of Mannitol Salt Agar in differentiating between bacterial species?
Presence of phenol red
How does Eosin Methylene Blue (EMB) Agar differentiate between Gram-negative and Gram-positive organisms?
Presence of eosin and methylene blue stains
Which technique is specifically used for the identification of antibiotic resistance like mecA?
Genomic PCR
What type of antibodies are required for the Direct ELISA method?
Monoclonal antibodies
Which staining method is commonly used for endospore staining?
Malachite Green Stain
What type of bacteria are mainly targeted by Ziehl–Neelsen stain?
Acid-fast bacteria
Which staining method is commonly used to detect polysaccharides in fungi?
Periodic acid-Schiff stain
In which type of microscopy is Gram Staining the first step in identifying pure bacterial cultures?
Transmission electron microscopy (TEM)
Which biochemical test can differentiate between bacteria that belong to Pseudomonadaceae and Enterobacteriaceae?
Oxidase test
Which technique is commonly used for the definitive identification of bacterial species?
Whole Genome Sequencing
What method is increasingly used for identifying microbial contaminants due to its speed and lower cost compared to conventional methods?
MALDI TOF
Which agar can be used to identify bacterial species from selective media?
MacConkey Agar
What is the need for balancing between turnaround time and sensitivity in microbial identification techniques?
To optimize efficiency
Which method can provide information about resistance in microbial samples?
MALDI TOF
In microbial identification, which of the following databases exist for different bacteria species?
Which chapter of 'Hugo and Russell’s Pharmaceutical Microbiology' discusses the ideal diagnostic characteristics including accuracy, sensitivity, cost-effectiveness, and speed?
Chapter 3 Section 8
What is the main purpose of using selective media such as Mannitol Salt Agar in microbial identification?
To inhibit the growth of unwanted bacteria while allowing the growth of target bacteria
In microbial identification, what is the primary purpose of using MacConkey Agar?
To differentiate between Gram-positive and Gram-negative bacteria based on lactose fermentation
What is the significance of Baird-Parker Agar in microbiology?
It is employed in the isolation and enumeration of Staphylococci, particularly Staphylococcus aureus
Sabouraud Dextrose Agar is primarily used for which type of microorganism identification?
Yeast and molds
Which statement best describes the role of differential media in microbial identification?
Differential media help in distinguishing between different species or types of microorganisms based on specific biochemical reactions.
How do selective and differential media complement each other in microbial identification?
Selective media inhibit unwanted bacteria, while differential media help differentiate between species based on colony morphology.
Test your knowledge on selective and differential media in microbiology, including their characteristics and examples like MacConkey Agar, Sabouraud Dextrose Agar, and Baird-Parker Agar.
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