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Questions and Answers
55.2% of tissue culture trials were published between 2006 and 2010.
True
Tissue culture research was more focused on woody plants than herbaceous plants.
False
The recovery of pathogen-free clones from infected plants is based on the premise that pathogen concentration is uniform throughout the infected plant.
False
Phloem-limited viruses are unable to invade callus derived from anther and ovary cultures.
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Thermotherapy is the most frequent technique for sanitation over the last 20 years.
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Nicotiana benthamiana plants infected by Cassava mosaic disease showed a close relationship between temperature and VIGS.
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Qu et al. (2005) focused on the involvement of RNA-dependent RNA polymerase (RdRp) in N. benthamiana plants infected by Potato virus X.
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Wang et al. (2008) conducted combined thermotherapy and cryotherapy treatments on raspberry plants infected by Raspberry dwarf virus.
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The development of research in the field of chemotherapy has been more lively than the work conducted on thermotherapy.
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The discovery of ribavirin represented a defining moment in the research of new chemical synthesis analogues of nucleoside or precursors of RNA bases.
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Chemotherapy trials for woody plants were more frequently conducted on herbaceous plants.
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Oseltamivir showed positive results when applied to Nicotiana tabacum infected with CMV.
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More than 50 antiviral molecules are available in the market for clinical application.
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Experiments on phytoviruses have not highlighted the effectiveness of antiviral drugs in the botanical field.
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IMPDH inhibitors target the inosine monophosphate dehydrogenase enzyme to inhibit the production of viral nucleic acid.
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Thermotherapy involves using low temperatures to eliminate viruses in plants.
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SAH hydrolase inhibitors prevent viral RNA maturation by preventing the conversion of S-adenosylhomocysteine to homocysteine and adenosine.
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NA inhibitors block viral spread by preventing the release of newly-generated virion progeny from infected cells.
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Plant tissue culture is a technique used to grow large plant parts in artificial media.
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The success of virus elimination using tissue culture and somatic embryogenesis depends on the genetic variations in the virus and the plant tissue.
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Study Notes
- Text discusses various antiviral strategies for eliminating viruses in plants, focusing on thermotherapy, SAH hydrolase inhibitors, and NA inhibitors.
- Thermotherapy involves using high temperatures to eliminate viruses in plants such as Artichoke, Begonia, Gladiolus, Onion, Peanut, Sweet Potato, Ulluco, and Cymbidium by reducing the intracellular pool of guanosine and inhibiting viral RNA synthesis.
- SAH hydrolase inhibitors prevent viral RNA maturation by preventing the conversion of S-adenosylhomocysteine to homocysteine and adenosine, making it impossible for viral RNA to form.
- NA inhibitors block viral spread by preventing the release of newly-generated virion progeny from infected cells, and two commercially available NA inhibitors are zanamivir and oseltamivir.
- Plant tissue culture is a technique used to grow small plant parts in artificial media and can be used to produce virus-free plants by eliminating viruses from the explant material.
- The size and type of tissue used in plant tissue culture affect the success of virus elimination, with smaller tissue sizes requiring longer culture times and higher virus concentrations.
- Somatic embryogenesis, a technique used to regenerate plantlets from callus tissue, has been used to eliminate viruses from plants, with the success rate depending on the length of callus induction and the characteristics of the callus.
- The efficiency of virus elimination using tissue culture and somatic embryogenesis depends on various factors, including the virus distribution in the plant tissue, the mechanisms of virus movement, and the genetic variations in the virus and the plant tissue.
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Description
Test your knowledge of scientific research references with this quiz. Identify the authors and publication years of well-known studies and papers.
