LAT Chapter 12 Rs Methodology

Which of the following best describes the role of laboratory animal technicians in research studies?

What is the goal of toxicology studies in animal research?

What are the areas of expertise required for toxicologists?

Which type of radiation exposure is accomplished using lead shields to protect certain parts of the body?

What is the most common source of radiation used in gamma irradiators for experimental purposes?

What has enabled researchers to create mice with specific immune system genes either turned off, overexpressed, induced, or conditionally expressed?

Which type of immunodeficiency model is an excellent model for investigating cancers?

Which type of mice lack natural killer (NK) cells?

Which method of induced immunodeficiency is faster and more easily controlled compared to other methods?

Which type of mice have a hereditary B cell defect?

Which type of immune cells are used in the Limulus amebocyte lysate (LAL) test?

What is the purpose of using immunodeficient animals in research?

What is the role of T cells in the immune system?

What is the significance of the nude mutation in mice?

What is the purpose of the Draize Test?

What is the difference between subacute and subchronic studies?

What is the typical number of animals per sex in a subacute study group?

What is the preferred non-rodent model for toxicity studies?

What is the controversy surrounding the Draize Test?

What is the purpose of satellite animals in toxicity studies?

How many rodents per sex per group are required for chronic studies?

What is the purpose of reproductive and developmental toxicity studies?

What is the purpose of Segment II studies in reproductive and developmental studies?

What is the Limulus amebocyte lysate (LAL) test used for?

What is the purpose of Segment III studies in reproductive and developmental studies?

What is a pyrogen test used for?

Which of the following is true regarding acute toxicity studies?

Which of the following is NOT a way to categorize toxicity tests?

What is irritancy in toxicology?

What is the purpose of acute toxicity testing?

Animal research is solely focused on finding new cures for human diseases.

Laboratory animal technicians are not involved in the conduct of a study or test.

Toxicologists only need to be familiar with the science of poisons and their effects on living organisms.

Acute toxicity tests involve exposure to a toxicant for a short period, usually less than 24 hours

Subchronic tests involve administration of a single dose of the toxic substance

Carcinogenesis refers to the induction of mutations in the DNA

The OECD guidelines for acute toxicity testing have replaced the LD50 test with methods that use more animals

SCID mice are unable to produce any immune system cells.

SCID mice are unable to mount either a cell-mediated or an antibody response to infection.

Chemotherapy drugs used to suppress immunity interfere with DNA or RNA synthesis.

Induced tolerance is the only method of introducing immunodeficiencies into animal models.

Subchronic studies typically require 10 rodents or 3 non-rodents per sex per group.

Recovery animals are used to assess the reversibility of any changes from toxic effects during dosing.

Blood microsampling techniques can eliminate the use of satellite animals for the purpose of toxicokinetic analyses.

Chronic studies require more animals per sex per group than subchronic studies.

Is a gamma irradiator the most common type of irradiation equipment for experimental purposes?

Is low-dose semi-continuous radiation a continuous exposure to radiation?

Is it necessary to have specially trained operators and a radiation safety program when handling irradiation equipment?

True or false: The Limulus amebocyte lysate (LAL) test is used as an alternative to rabbit assays for detecting bacterial toxins in medical products?

True or false: Monocyte activation tests can detect both nonbacterial pyrogens and a wider variety of bacterial toxins?

True or false: Immunodeficient animals are incapable of rejecting transplants or tissue?

True or false: Nude mice lack a thymus gland and therefore cannot develop T cells, making them T cell-deficient?

Reproductive toxicity studies are only conducted in rodents.

Segment II studies evaluate fetal toxicity in a rodent and a non-rodent species.

Pyrogen tests involve administering the product intravenously to several animals and recording their body temperature.

The Limulus amebocyte lysate test uses the hemolymph of the horseshoe crab to detect bacterial toxins.

True or false: The Draize test is no longer commonly used due to its subjectivity and lack of reproducibility?

True or false: In vitro testing using three-dimensional models of the skin and cornea have been extensively validated and have correlated well with the Draize test?

True or false: Developing models of acute toxicity using ex vivo and in vitro testing is challenging due to the complexity of both the skin and eye?

True or false: Global regulatory testing guidelines generally require studies in one rodent and one nonrodent species, with rats, dogs, or nonhuman primates being the preferred non-rodent models?