SCCG 3673 Microbial Growth Requirements
21 Questions
0 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to Lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

Which of the following environments can be a source of thermophiles?

  • Desert sand
  • Snowy mountains
  • Tropical rainforests
  • Lake sediments (correct)
  • What temperature is mentioned as suitable for the growth of thermophilic organisms in Crater Lake, White Island?

  • 50°C (correct)
  • 100°C
  • 25°C
  • 10°C
  • What is a characteristic of the orange cyanobacteria found around the geyser at Tokaanu?

  • They appear only in deep water.
  • They require acidic conditions to grow.
  • They thrive in cooler temperatures.
  • They flourish in the warmer splash zone. (correct)
  • What shape is primarily described in relation to the sinter at Opaheke?

    <p>Unusual and formed by thermal water (B)</p> Signup and view all the answers

    Which source of thermophiles has a recorded temperature of 350°C?

    <p>Hydrothermal vents (D)</p> Signup and view all the answers

    Which group is classified as hyperthermophiles that oxidize H2 or reduced sulfur compounds?

    <p>Aquifex-Hydrogenobacter (C)</p> Signup and view all the answers

    Which type of organism is Thermatoga classified as?

    <p>Chemoorganotroph (A)</p> Signup and view all the answers

    Which organism is known for thriving in extremely acidic conditions?

    <p>Picrophilus torridus (D)</p> Signup and view all the answers

    Where can Thermophiles be found in unheated substrates?

    <p>Bird's nest (B)</p> Signup and view all the answers

    Which temperature range is considered for substrates spontaneously heated?

    <p>60 to 70ºC (C)</p> Signup and view all the answers

    What type of extremophiles are most kingdoms in Archae classified as?

    <p>Thermophiles or hyperthermophiles (A)</p> Signup and view all the answers

    What is the optimum temperature range for thermophilic microorganisms?

    <p>45 - 80°C (C)</p> Signup and view all the answers

    Which of the following is NOT classified as a thermophilic microorganism?

    <p>Escherichia coli (D)</p> Signup and view all the answers

    Which of the following organisms is NOT associated with geothermal heating environments?

    <p>Lactobacillus (D)</p> Signup and view all the answers

    How do hyperthermophiles survive at extremely high temperatures?

    <p>By utilizing antioxidants to stabilize proteins (A)</p> Signup and view all the answers

    Which region is known for having a wide range of thermophilic environments?

    <p>United States (D)</p> Signup and view all the answers

    The classification of organisms that grow optimally between 55°C and 80°C is known as?

    <p>Thermophiles (A)</p> Signup and view all the answers

    Which of the following groups contains microorganisms that can be classified as thermophiles?

    <p>Gram-negative aerobes (A)</p> Signup and view all the answers

    What distinguishes extreme thermophiles from regular thermophiles?

    <p>Their optimum growth temperature is above 80°C (A)</p> Signup and view all the answers

    Where are thermophilic microorganisms most commonly found?

    <p>In hot springs and volcanic areas (D)</p> Signup and view all the answers

    What characteristic is primarily used to classify thermophilic microorganisms?

    <p>Their requirement for high temperature growth (A)</p> Signup and view all the answers

    Flashcards

    Thermophiles

    Microorganisms that thrive at high temperatures, 45-80°C.

    Psychrophilic

    Microbes that grow at 0 to 20°C, optimal at 10°C.

    Mesophilic

    Moderate-temperature-loving microbes, growing at 20 to 40°C, optimal around 37°C.

    Extreme thermophiles

    Also known as hyperthermophiles, survive above 80°C, often found in hot springs.

    Signup and view all the flashcards

    Thermophile classification

    Groups based on temperature preference: psychrophilic, mesophilic, thermophilic, extreme thermophiles.

    Signup and view all the flashcards

    Substrate treatments

    Methods used to isolate thermophiles from their environment.

    Signup and view all the flashcards

    Isolation techniques

    Various methods employed to isolate thermophilic microorganisms.

    Signup and view all the flashcards

    Role of thermophiles

    Significant for industrial and environmental biotechnology applications.

    Signup and view all the flashcards

    Sungai Klah Hot Springs

    A location in Perak known for its hot springs and thermophilic organisms.

    Signup and view all the flashcards

    Hydrothermal Vents

    Locations on the ocean floor where heated water and minerals seep out, fostering extremophilic life.

    Signup and view all the flashcards

    Cyanobacteria

    Photosynthetic bacteria that can thrive in extreme environments like hot springs.

    Signup and view all the flashcards

    Opaheke Sinter

    A sinter formation shaped by thermal water and thermophilic bacteria at Opaheke, characterized by unusual shapes.

    Signup and view all the flashcards

    Hottest Thermophile

    A thermophile that thrives at extreme temperatures, currently holding the record for the highest heat tolerance.

    Signup and view all the flashcards

    Kingdoms of Thermophiles

    Thermophiles are classified into three kingdoms: Eubacteria, Archaebacteria, and Eukarya.

    Signup and view all the flashcards

    Hyperthermophiles

    Organisms, like Aquifex or Thermotoga, that thrive in extremely high temperatures, often over 90°C.

    Signup and view all the flashcards

    Sources of Thermophiles

    Thermophiles can be found in hot springs, geothermal soils, and even composting materials.

    Signup and view all the flashcards

    Aquifex-Hydrogenobacter

    A hyperthermophilic group of bacteria that oxidizes hydrogen or reduced sulfur compounds at high temperatures.

    Signup and view all the flashcards

    Thermus aquaticus

    A thermophilic bacterium commonly found in hot springs, known for its heat-stable enzymes used in PCR.

    Signup and view all the flashcards

    Sulfolobus

    An extremophilic genus of crenarchaeota that thrives in acidic hot springs and emits sulfur.

    Signup and view all the flashcards

    Geothermal Regions

    Areas such as the USA, Japan, and Iceland known for hosting thermophilic organisms due to heat.

    Signup and view all the flashcards

    Study Notes

    Course Information

    • Course Title: Physiology and Screening of Industrial Microorganisms
    • Course Code: SCCG 3673
    • Academic Year: 2024/2025
    • Semester: 1
    • Instructor: Dr. Praseetha Prabhakaran
    • Email: [email protected]
    • Location: T02, Level 4

    Chapters

      1. Introduction
      1. Microbial Growth Requirements
      1. Methods of Isolating Pure Cultures
      1. Screening of Anaerobic Microorganisms
      1. Screening of Thermophilic Microorganisms
      1. Screening of Alkalophilic Microorganisms
      1. Screening of Acidophilic Microorganisms

    Learning Objectives

    • Explain microbial classification based on temperature requirements
    • Define psychrophilic, mesophilic, and thermophilic microbes
    • Describe major groups of thermophilic microbes and their environmental locations
    • Explain how thermophiles and hyperthermophiles survive at high temperatures
    • Detail substrate treatments and isolation techniques for thermophiles
    • Describe the industrial and environmental applications of thermophiles

    Thermophiles

    • Microorganisms capable of growth at high temperatures
    • Optimal temperature range: 45°C to below 80°C
    • Isolated from various prokaryotic groups (e.g., cyanobacteria, gram-negative aerobes, photosynthetic bacteria, spore formers, lactic acid bacteria, methane producers, sulfur oxidizers/sulfate reducers, mycoplasma, Actinomycetes, Pseudomonas)

    Temperature Optimum for Thermophiles

    • Thermophiles: Optimal temperature (Topt) ≥ 45°C
    • Hyperthermophiles: Optimal temperature (Topt) ≥ 80°C
    • Extreme thermophiles: Optimal temperature (Topt) ≥ 70°C
    • Moderate thermophiles: Optimal temperature (Topt) 45-70°C

    Classification of Thermophiles

    • Psychrophilic: -10°C to 20°C (cold-loving)
    • Psychrotrophs: 0°C to 30°C (opt 20°C)
    • Mesophilic: 20°C to 40°C (moderate-temp-loving)
    • Thermophiles: 55°C to 80°C (heat-loving)
    • Extreme thermophiles/Hyperthermophiles: >80°C, often above 80°C

    Sources of Thermophiles

    • Hot springs and associated soils (near boiling point or above)
    • Geothermally heated soil
    • Submarine thermal vents
    • Spontaneously heated substrate (60-70°C) (e.g., mushroom compost, municipal compost, sewage, composted sewage, bird's nests, power station effluent)
    • Unheated substrate (50°C) (e.g., soil, peat, lake/river sediments, marine sediments, copper mine leach dumps, house dust, air, sugar)
    • Oil wells, Suwa Hotspring (Japan), Green Dragon Spring (Yellowstone National Park), waterlogged soil, Crater Lake, White Island, Sinter, Opaheke, Tokaanu geyser(s).

    Characteristics of Thermophilic Proteins

    • More ion pairs
    • Greater average hydrophobicity of interior residues
    • More compact core containing disulfide bonds
    • More hydrogen bonds
    • Structurally tolerant and highly evolvable (compared to mesophilic proteins)

    Studying That Suits You

    Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

    Quiz Team

    Related Documents

    Thermophiles Part 1 PDF

    Description

    This quiz covers the key concepts in microbial growth requirements as discussed in the Physiology and Screening of Industrial Microorganisms course. You will explore different types of microorganisms, their classifications based on temperature, and the methods for isolating pure cultures. Test your knowledge on the environmental applications of thermophilic, psychrophilic, and other groups of microbes.

    More Like This

    Microbial Growth Factors
    67 questions

    Microbial Growth Factors

    ManeuverableForgetMeNot2590 avatar
    ManeuverableForgetMeNot2590
    Microbial Growth Categories Quiz
    20 questions
    Microbial Growth in Food Environments
    203 questions
    Microbial Growth Phases Quiz
    34 questions

    Microbial Growth Phases Quiz

    ConsummateConnemara7771 avatar
    ConsummateConnemara7771
    Use Quizgecko on...
    Browser
    Browser