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Scanning Electron Microscopy (SEM) Quiz

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Questions and Answers

What does the instrumentation of SEM consist of?

Anode with a negative charge and a positively charged electrode.

A mechanism for penetrating the sample at different micron levels.

Source, objective lens, and an electrode that deflects electrons. (correct)

A mechanism for capturing differential images and software for compiling images.

What is the primary advantage of SEM over TEM?

SEM is better suited for sub-cellular structures.

SEM provides a 3D view of the specimen's surface. (correct)

SEM has a higher resolution than TEM.

SEM uses a similar instrumentation to the light microscope.

What is the principle of SEM based on?

Capturing differential images using a CMOS sensor.

Rotating the specimen to cover all its surfaces.

Using a positively charged electrode to deflect electrons toward a specific area.

Scattering electrons and scanning the specimen based on its different depth of field. (correct)

How are electrons deflected in SEM?

<p>A negatively charged anode deflects electrons to the objective lens.</p> Signup and view all the answers

What is the advantage of SEM in understanding materials like drugs and salt crystals?

<p>SEM provides a crisp and detailed image with higher resolution.</p> Signup and view all the answers

Study Notes

Scanning Electron Microscopy (SEM)

SEM instrumentation consists of an electron gun, scanning coils, detectors, and a vacuum system.

Advantage of SEM over TEM

The primary advantage of SEM over TEM is its ability to image surfaces of samples, whereas TEM is limited to imaging thin sections.

Principle of SEM

The principle of SEM is based on the scanning of a focused beam of electrons over a sample, which interacts with the sample, producing signals that contain information about the sample's surface topography.

Electron Deflection in SEM

In SEM, electrons are deflected by electrostatic or magnetic fields to scan the sample in a raster pattern.

Advantage of SEM in Understanding Materials

The advantage of SEM in understanding materials like drugs and salt crystals is that it allows for the study of their surface morphology, which is crucial in understanding their properties and behavior.

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Description

Test your knowledge of scanning electron microscopy (SEM) with this quiz. Explore the instrumentation and applications of SEM, and learn about its capabilities in visualizing 3D specimens at high resolution.