SAP Enzyme in Molecular Biology

BAP hydrolyzes 3’ and 5’ phosphate from nucleic acid (DNA/RNA), removing phosphate groups before end labeling and from vectors prior to insert ligation.

CIP is used to remove phosphate groups from the 5’ end of DNA and RNA, preventing self-ligation and recircularization of vectors.

Inorganic phosphates reduce BAP activity.

<p>SAP is highly specific and heat-labile.</p> Signup and view all the answers

<p>65°C</p> Signup and view all the answers

<p>Dephosphorylation prevents self-ligation and recircularization of vectors, improving the efficiency of vector constructs containing desired inserts.</p> Signup and view all the answers

<p>Phosphomonoester</p> Signup and view all the answers

<p>Calf intestine</p> Signup and view all the answers

<p>To prevent self-ligation and recircularization of vectors.</p> Signup and view all the answers

<p>BAP is a thermostable enzyme that can hydrolyze 3’ and 5’ phosphate from nucleic acid (DNA/RNA).</p> Signup and view all the answers