Sample Collection Procedures for Veterinary Medicine
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Questions and Answers

What is the recommended volume of formalin to be used for a tissue biopsy when performing fine needle aspiration?

  • Equal volume to the specimen
  • 20 times the volume of the specimen
  • 5 times the volume of the specimen
  • 10 times the volume of the specimen (correct)

Which type of needle is recommended for performing fine needle aspiration?

  • 22-25G needle (correct)
  • 30-32G needle
  • 20-22G needle
  • 16-18G needle

What is the purpose of a vaginal cytology examination?

  • To detect infections
  • To assess the level of hydration
  • To measure hormone levels
  • To identify the estrus stage (correct)

What condition does an enema primarily address?

<p>Impaction (constipation) (B)</p> Signup and view all the answers

What type of sample is collected during bronchoalveolar lavage?

<p>Fluid from the trachea and bronchi (D)</p> Signup and view all the answers

Flashcards

Urine Collection

Collecting urine samples, typically 10cm in length, and placing them in EDTA for analysis.

Bone Marrow Collection

Obtaining bone marrow using a Rosenthal needle in a sterile procedure. New methylene blue is used for analysis.

Vaginal Cytology

Examining cells from the vagina, checking the estrus phase. Different cell types are categorized.

Fine Needle Aspiration

Collecting tissue samples from masses using a thin needle (22-25G) and syringe (3-12 ml).

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Formalin Fixation

Preserving tissue samples by immersing them in formalin (10x volume).

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Abdomen Wall Insertion

Inserting a needle to access the bladder.

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Arthrocentesis

A procedure of collecting fluid from a joint.

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Bronchoalveolar Lavage

Collecting samples from the trachea and bronchi.

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Nasal Flush/Bronchoscopy

Collecting samples from the nasal cavity; bronchoscopy is a technique to examine the bronchi.

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Enema (Constipation)

Treatment for constipation, re-establishing normal bowel function.

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Enema Types

Different types include warm water, soap, mineral oil, lubricating, using a syringe or funnel.

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Study Notes

Sample Collection Procedures

  • General Sample Collection: Collect samples from skin, ears, mouth, nasopharynx, oropharynx, stomach, small intestine, and colon.
  • Tissue Biopsy: Aspirate tissues using a fine needle and a 22-259 gauge needle. Collect 3-12 ml of tissue sample and place in formalin for preservation. (Use 10x specimens.)
  • Urine Collection: Collect urine samples.
  • Bone Marrow Collection: Aspirate bone marrow using a Rosenthal needle. Appropriately sample the marrow.
  • Vaginal Cytology: Examine cells from the vagina to determine estrus stages (parabasal, intermediate, superficial, and atypical).

Centesis Procedures

  • Abdomen Wall Insertion: Insert a needle through the abdomen wall into the bladder to collect urine.
  • Arthrocentesis: Collect synovial fluid from a joint (e.g., knee).
  • Bronchoalveolar Lavage: Use a bronchoscope to lavage the tracheal, bronchial, and nasal cavities, which are common sites for sample collection.
  • Nasal Flush: Flush and collect samples from the nasal cavity.

Enema Types

  • Warm Water Enema: Administer warm water enemas, which are water-based.
  • Soap Enema: Use soap for enemas.
  • Mineral Oil Enema: Employ mineral oil for enemas.
  • Lubricant Enemas: Utilize lubricants or tubing.
  • Medication Enemas: Add medications to enemas for specific needs.

Other Information

  • Specific Procedures: There is information regarding insertion techniques, materials, measurements, time estimations, and types of catheters. Equipment used include, but not limited to, red rubber (French), PVC, Foley catheters, and PTFE materials.
  • Sample Preservation: Samples should be placed in proper solutions (EDTA, formalin).
  • Sample Handling: Important steps regarding sample handling and procedures are noted within the text.
  • Procedure Variations: Information details variations in procedures and collection methods.
  • Associated Conditions: Collect samples relevant to specific disease-associated conditions like peritonitis and obstruction, and their respective procedures.

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Description

This quiz covers various sample collection techniques essential in veterinary practice, including general sample collection from different body parts, tissue biopsies, urine collection, and specialized procedures like bronchoalveolar lavage. Test your knowledge on the correct methods and tools used in these processes.

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