5 Questions
What is the primary reason for the emergence of reversed-phase high-performance liquid chromatography (RP-HPLC) as an important tool for the evaluation of protein molecules and peptides?
Outstanding resolutions are feasible for closely related or structurally distinct molecules of interest.
What percentage of analytical chromatographic techniques use reversed-phase substances for separations today?
Over 80%
Which type of substances have been effectively used in various applications, including the quantitative detachment of drugs and retrieval of pollutants from samples?
Reversed-phase substances
Why has RP-HPLC appeared as the technique of choice for treating HPLC-based physical separation?
It allows outstanding resolutions for molecules of interest.
What aspect of RP-HPLC facilitates separations and is particularly beneficial for both quantitative and specific applications?
Gradient phase separation
Learn about the applications of reversed-phase high-performance liquid chromatography (RP-HPLC) in protein and peptide evaluation. Understand its benefits in terms of stability testing, reproducibility, resolution, recoveries, and efficiency.
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