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Questions and Answers
What is the primary reason for the emergence of reversed-phase high-performance liquid chromatography (RP-HPLC) as an important tool for the evaluation of protein molecules and peptides?
What is the primary reason for the emergence of reversed-phase high-performance liquid chromatography (RP-HPLC) as an important tool for the evaluation of protein molecules and peptides?
- Matrix's stability was tested under a range of mobile phase scenarios.
- Outstanding resolutions are feasible for closely related or structurally distinct molecules of interest. (correct)
- Reversed-phase substances are more adaptable than normal-phase adsorbents.
- Significant recoveries and efficiency in gradient phase separation.
What percentage of analytical chromatographic techniques use reversed-phase substances for separations today?
What percentage of analytical chromatographic techniques use reversed-phase substances for separations today?
- Around 60%
- Approximately 40%
- Less than 20%
- Over 80% (correct)
Which type of substances have been effectively used in various applications, including the quantitative detachment of drugs and retrieval of pollutants from samples?
Which type of substances have been effectively used in various applications, including the quantitative detachment of drugs and retrieval of pollutants from samples?
- Normal-phase adsorbents
- Biological molecules like proteins and peptides
- Reversed-phase substances (correct)
- Metabolic products and energetic biochemicals
Why has RP-HPLC appeared as the technique of choice for treating HPLC-based physical separation?
Why has RP-HPLC appeared as the technique of choice for treating HPLC-based physical separation?
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What aspect of RP-HPLC facilitates separations and is particularly beneficial for both quantitative and specific applications?
What aspect of RP-HPLC facilitates separations and is particularly beneficial for both quantitative and specific applications?
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