RP-HPLC in Protein Analysis
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Questions and Answers

What is the primary reason for the emergence of reversed-phase high-performance liquid chromatography (RP-HPLC) as an important tool for the evaluation of protein molecules and peptides?

  • Matrix's stability was tested under a range of mobile phase scenarios.
  • Outstanding resolutions are feasible for closely related or structurally distinct molecules of interest. (correct)
  • Reversed-phase substances are more adaptable than normal-phase adsorbents.
  • Significant recoveries and efficiency in gradient phase separation.

    • What percentage of analytical chromatographic techniques use reversed-phase substances for separations today?

  • Around 60%
  • Approximately 40%
  • Less than 20%
  • Over 80% (correct)

    • Which type of substances have been effectively used in various applications, including the quantitative detachment of drugs and retrieval of pollutants from samples?

  • Normal-phase adsorbents
  • Biological molecules like proteins and peptides
  • Reversed-phase substances (correct)
  • Metabolic products and energetic biochemicals

    • Why has RP-HPLC appeared as the technique of choice for treating HPLC-based physical separation?

    <p>It allows outstanding resolutions for molecules of interest.</p> Signup and view all the answers

    What aspect of RP-HPLC facilitates separations and is particularly beneficial for both quantitative and specific applications?

    <p>Gradient phase separation</p> Signup and view all the answers

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