RNA Polymerases and Alternative Splicing
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Questions and Answers

Which RNA polymerase is responsible for transcribing protein-encoding genes in eukaryotes?

  • RNA polymerase III
  • RNA polymerase II (correct)
  • RNA polymerase I
  • RNA polymerase IV
  • How many RNA polymerases do eukaryotes possess?

  • Two
  • Four
  • Three (correct)
  • One
  • What type of genes does RNA polymerase I transcribe?

  • 5S rRNA genes
  • rRNA genes (correct)
  • tRNA genes
  • mRNA genes
  • Which of the following RNA polymerases is NOT found in prokaryotes?

    <p>RNA polymerase I</p> Signup and view all the answers

    Which function does RNA polymerase III perform?

    <p>Transcribes tRNA genes and 5S rRNA genes</p> Signup and view all the answers

    What is the primary outcome of alternative splicing in RNA molecules?

    <p>Creation of multiple proteins from the same RNA</p> Signup and view all the answers

    Which statement best describes the role of alternative splicing in biological processes?

    <p>It enhances the complexity of gene expression.</p> Signup and view all the answers

    How does alternative splicing contribute to diagnostics and therapeutics?

    <p>It helps identify novel diagnostic and prognostic biomarkers.</p> Signup and view all the answers

    What does the diagram of alternative splicing mainly illustrate?

    <p>Different proteins arise from varied exon combinations.</p> Signup and view all the answers

    In the context of alternative splicing, which describes its significance for organism development?

    <p>It plays a vital role in cellular differentiation.</p> Signup and view all the answers

    What enzyme is primarily responsible for the synthesis of pre-mRNA during transcription?

    <p>RNA polymerase II</p> Signup and view all the answers

    During transcription, what is the relationship between the newly formed mRNA and the original DNA sequence?

    <p>They have complementary sequences.</p> Signup and view all the answers

    What is the process called that modifies pre-mRNA into mature mRNA?

    <p>RNA splicing</p> Signup and view all the answers

    In the context of gene expression, what role does mRNA play after its formation?

    <p>It carries information for protein synthesis.</p> Signup and view all the answers

    What represents the primary outcome of the transcription process?

    <p>Production of pre-mRNA</p> Signup and view all the answers

    What does the codon AUG specify in the process of translation?

    <p>Amino acid that signals the start of translation</p> Signup and view all the answers

    Which of the following codons is considered a termination codon?

    <p>UAA</p> Signup and view all the answers

    How are codons structured in mRNA?

    <p>Group of three nucleotides</p> Signup and view all the answers

    What is the primary function of RNA polymerase during the elongation stage of transcription?

    <p>To synthesize a complementary RNA strand using a DNA template</p> Signup and view all the answers

    In the codon table, what amino acid is encoded by the codon UUU?

    <p>Phenylalanine</p> Signup and view all the answers

    What role does the genetic code play in translation?

    <p>It determines the sequence of amino acids in a protein</p> Signup and view all the answers

    Which base pairing occurs between the DNA template and the RNA during transcription?

    <p>A-U and C-G pairing</p> Signup and view all the answers

    In which step does RNA polymerase bind to the promoter region of the gene?

    <p>Initiation</p> Signup and view all the answers

    What happens to the DNA double helix after transcription is complete?

    <p>It reforms into its original double helix structure</p> Signup and view all the answers

    What signals the termination of transcription in the presence of RNA polymerase?

    <p>The encounter with a codon that acts as a stop signal</p> Signup and view all the answers

    What role do snRNPs play in the process of mRNA splicing?

    <p>They bind to the intron sequence and facilitate its removal.</p> Signup and view all the answers

    What structural formation is created from the folding of the intron during splicing?

    <p>A lariat loop.</p> Signup and view all the answers

    What happens to the exons during mRNA splicing?

    <p>They move closer together and join to form the mature mRNA.</p> Signup and view all the answers

    What is released as a byproduct of the splicing process?

    <p>The intron.</p> Signup and view all the answers

    After performing its function in splicing, what can snRNPs do?

    <p>Detach and be reused for further splicing events.</p> Signup and view all the answers

    What is the primary function of the 5' cap in pre-mRNA processing?

    <p>Protects mRNA from degradation</p> Signup and view all the answers

    Which of the following accurately describes the role of the 3' poly-A tail?

    <p>It protects mRNA from degradation</p> Signup and view all the answers

    During pre-mRNA splicing, what is primarily removed from the transcript?

    <p>Introns</p> Signup and view all the answers

    How do end modifications affect the stability of mRNA?

    <p>They increase the stability of the mRNA</p> Signup and view all the answers

    What essential role does splicing play in pre-mRNA processing?

    <p>It ensures the correct sequence by removing non-coding regions</p> Signup and view all the answers

    What direction does transcription proceed in?

    <p>5' – 3'</p> Signup and view all the answers

    In which direction is the antisense (template) strand read during transcription?

    <p>3' – 5'</p> Signup and view all the answers

    Which of the following statements is true about the newly generated RNA strand?

    <p>It is complementary to the template strand.</p> Signup and view all the answers

    Which end of the RNA strand is extended by RNA polymerase during transcription?

    <p>3' end</p> Signup and view all the answers

    Which of the following statements accurately describes the relationship between mRNA and the template strand?

    <p>mRNA is complementary to the template strand.</p> Signup and view all the answers

    Which type of RNA is primarily responsible for carrying information from DNA to ribosomes?

    <p>Messenger RNA (mRNA)</p> Signup and view all the answers

    What is the main role of transfer RNA (tRNA) in protein synthesis?

    <p>Transporting amino acids to the mRNA strand</p> Signup and view all the answers

    Which statement accurately describes ribosomal RNA (rRNA)?

    <p>It is part of the ribosome and aids in assembling amino acids.</p> Signup and view all the answers

    Which of the following describes the structure of transfer RNA (tRNA)?

    <p>A three-legged structure with colored attachments</p> Signup and view all the answers

    What role does messenger RNA (mRNA) play in the process of protein synthesis?

    <p>It acts as a template for assembling a sequence of amino acids.</p> Signup and view all the answers

    Which of the following accurately describes a key difference between DNA and RNA?

    <p>The sugar in DNA is deoxyribose, whereas RNA contains ribose.</p> Signup and view all the answers

    Which nitrogenous base is unique to RNA as compared to DNA?

    <p>Uracil</p> Signup and view all the answers

    What is the primary structural characteristic of RNA that distinguishes it from DNA?

    <p>RNA is usually single-stranded.</p> Signup and view all the answers

    Which statement best summarizes the functional relationship between DNA and RNA?

    <p>RNA synthesizes proteins using the information encoded in DNA.</p> Signup and view all the answers

    How do the sugars of RNA and DNA differ structurally?

    <p>RNA has a carbon atom at position 2 that is absent in DNA.</p> Signup and view all the answers

    Study Notes

    RNA Polymerases: Eukaryotes vs. Prokaryotes

    • Prokaryotes: only one RNA polymerase
    • Eukaryotes: three RNA polymerases
      • RNA polymerase I transcribes rRNA genes
      • RNA polymerase II transcribes protein-encoding genes
      • RNA polymerase III transcribes tRNA genes and 5S rRNA genes

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    Description

    This quiz explores the functions and characteristics of RNA polymerases in eukaryotes and their role in transcription. Additionally, it delves into the process of alternative splicing and its significance in gene expression and organism development. Test your knowledge on these fundamental concepts in molecular biology.

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