T.11 Promotores para la RNA Pol II

What is the primary function of the initiator in the core promoter?

To enhance the specificity of transcription
To bind the RNA polymerase II
To locate the nucleotide where transcription begins (correct)
To recognize the TFIIB factor

What is the main difference between the promoter proximal and distal elements?

Their function in enhancing transcription
Their interaction with RNA polymerase II
Their location in the genome (correct)
Their evolutionary conservation

Which of the following is NOT a characteristic of the core promoter?

Presence of an initiator sequence
Presence of downstream promoter elements
Presence of a TATA box
Presence of enhancer elements (correct)

What is the primary function of the BRE element?

To recognize the TFIIB factor (B) Signup and view all the answers

Which of the following is a characteristic of promoter distal elements?

They act as enhancers or silencers at a distance (D) Signup and view all the answers

What is the difference between archaeal and eukaryotic promoters compared to bacterial promoters?

They are more similar in sequence and function (C) Signup and view all the answers

What is the factor that recognizes promoters in bacteria?

σ factor (C) Signup and view all the answers

What is the function of the TBP in transcription?

To bind to the TATA box (B) Signup and view all the answers

What is the percentage of promoters with a canonical TATA box?

20% (A) Signup and view all the answers

What is the function of histones in eukaryotes?

To compact DNA (A) Signup and view all the answers

What is the purpose of the Inr sequence?

To signal the start of transcription (D) Signup and view all the answers

What is the difference between the TSS and the Inr?

The TSS is the site of transcription initiation, while the Inr is the sequence that surrounds it (A) Signup and view all the answers

What is the purpose of the DPE element?

To position RNA Pol II correctly (B) Signup and view all the answers

What is the characteristic of TATA-less promoters?

They require TAFs for recognition (C) Signup and view all the answers

What is the similarity between the transcription systems of archaea and eukaryotes?

They are more similar than bacteria (C) Signup and view all the answers

What is the purpose of the TFIIB protein?

To bind to the BRE sequence (B) Signup and view all the answers

What percentage of sequences are TATA-like?

80% (D) Signup and view all the answers

What is the main difference between TATA and TATA-like sequences?

Their deformability (B) Signup and view all the answers

What is the characteristic of Sharp-Promoters (SP)?

They have fixed transcription start sites (C) Signup and view all the answers

What is the function of TAFs in promoters?

To help TBP recognize promoters (C) Signup and view all the answers

What is the characteristic of Broad-Promoters?

They have variable transcription start sites (B) Signup and view all the answers

What is the role of TBP in transcription?

To recognize TATA sequences (D) Signup and view all the answers

What is the difference between SP and BP?

SP has fixed TSS, BP has variable TSS (C) Signup and view all the answers

What is the function of the initiator (Inr) element?

To initiate transcription (A) Signup and view all the answers

What is the characteristic of promoters without TATA sequences?

They have variable transcription start sites (A) Signup and view all the answers

What is the function of the downstream core promoter element (DPE)?

To initiate transcription (D) Signup and view all the answers

What is the primary function of the TATA box in eukaryotic gene expression?

To activate transcription initiation (B) Signup and view all the answers

What is the main difference between a proximal promoter and a distal promoter?

Proximal promoters bind general transcription factors, while distal promoters bind specific transcription factors (D) Signup and view all the answers

What is the effect of deleting the TATA box on gene expression?

It decreases gene expression (A) Signup and view all the answers

What is the function of enhancers in gene regulation?

To increase transcription initiation (C) Signup and view all the answers

What is the characteristic of superenhancers?

They have a high affinity for transcription factors (C) Signup and view all the answers

What is the effect of deleting an enhancer on gene expression?

It decreases gene expression (D) Signup and view all the answers

What is the function of silencers in gene regulation?

To inhibit transcription initiation (D) Signup and view all the answers

What is the characteristic of promoters with respect to their distance from the gene?

They can be either upstream or downstream of the gene (B) Signup and view all the answers

What is the effect of deleting a silencer on gene expression?

It increases gene expression (D) Signup and view all the answers

What is the function of the BRE (TFIIB Recognition Element) in gene regulation?

To bind general transcription factors (A) Signup and view all the answers