Podcast
Questions and Answers
What is the purpose of using guanidinium thiocyanate (GTC) in RNA isolation?
What is the purpose of using guanidinium thiocyanate (GTC) in RNA isolation?
- To visualise RNA molecules on a nitrocellulose membrane
- To remove rRNA from eukaryotic mRNA
- To amplify mRNA for PCR
- To inhibit the activity of RNases (correct)
What is the primary function of the Poly A+ selection step in eukaryotic mRNA isolation?
What is the primary function of the Poly A+ selection step in eukaryotic mRNA isolation?
- To amplify the target RNA molecule
- To synthesise cDNA from mRNA
- To remove genomic DNA from the RNA sample
- To separate mRNA from rRNA (correct)
What is the purpose of the northern blotting technique?
What is the purpose of the northern blotting technique?
- To synthesize cDNA from mRNA
- To amplify target RNA molecules
- To isolate mRNA from eukaryotic cells
- To visualise and quantify RNA molecules (correct)
Which of the following is a characteristic of reverse transcription-PCR?
Which of the following is a characteristic of reverse transcription-PCR?
What is the purpose of the exponential phase in PCR?
What is the purpose of the exponential phase in PCR?
What is the function of SYBR Green in quantitative real-time PCR?
What is the function of SYBR Green in quantitative real-time PCR?
What is the purpose of using a dual-labelled probe in TaqMan PCR?
What is the purpose of using a dual-labelled probe in TaqMan PCR?
What is the purpose of making a cDNA library?
What is the purpose of making a cDNA library?
What is the purpose of ligating cDNA to a restriction enzyme-digested vector?
What is the purpose of ligating cDNA to a restriction enzyme-digested vector?
What is the purpose of selecting for bacteria with a cDNA insert?
What is the purpose of selecting for bacteria with a cDNA insert?
What is the primary purpose of adding guanidinium thiocyanate (GTC) to the RNA isolation process?
What is the primary purpose of adding guanidinium thiocyanate (GTC) to the RNA isolation process?
What is the primary benefit of using Northern analysis (blotting) over other RNA detection methods?
What is the primary benefit of using Northern analysis (blotting) over other RNA detection methods?
What is the primary function of the random hexamers in reverse transcription-PCR?
What is the primary function of the random hexamers in reverse transcription-PCR?
What is the primary advantage of using quantitative real-time PCR over traditional PCR?
What is the primary advantage of using quantitative real-time PCR over traditional PCR?
What is the primary purpose of creating a cDNA library?
What is the primary purpose of creating a cDNA library?
What is the primary benefit of using oligo dT primers in reverse transcription-PCR?
What is the primary benefit of using oligo dT primers in reverse transcription-PCR?
What is the primary purpose of the linear phase in PCR?
What is the primary purpose of the linear phase in PCR?
What is the primary benefit of using gene-specific primers in reverse transcription-PCR?
What is the primary benefit of using gene-specific primers in reverse transcription-PCR?
What is the primary purpose of transferring the RNA molecules to a nitrocellulose membrane in Northern analysis?
What is the primary purpose of transferring the RNA molecules to a nitrocellulose membrane in Northern analysis?
What is the primary benefit of using reverse transcription-PCR over traditional PCR?
What is the primary benefit of using reverse transcription-PCR over traditional PCR?
What is the primary reason why RNA isolation difficulties often arise?
What is the primary reason why RNA isolation difficulties often arise?
What is the primary advantage of using Poly A+ selection in eukaryotic mRNA isolation?
What is the primary advantage of using Poly A+ selection in eukaryotic mRNA isolation?
What is the primary purpose of transferring the RNA molecules to a nitrocellulose membrane in Northern analysis?
What is the primary purpose of transferring the RNA molecules to a nitrocellulose membrane in Northern analysis?
What is the primary function of the quencher in a TaqMan probe?
What is the primary function of the quencher in a TaqMan probe?
What is the primary advantage of using quantitative real-time PCR over traditional PCR?
What is the primary advantage of using quantitative real-time PCR over traditional PCR?
What is the primary purpose of making a cDNA library?
What is the primary purpose of making a cDNA library?
What is the primary function of the random hexamers in reverse transcription-PCR?
What is the primary function of the random hexamers in reverse transcription-PCR?
What is the primary advantage of using reverse transcription-PCR over traditional PCR?
What is the primary advantage of using reverse transcription-PCR over traditional PCR?
What is the primary purpose of ligating cDNA to a restriction enzyme-digested vector?
What is the primary purpose of ligating cDNA to a restriction enzyme-digested vector?
What is the primary function of the exponential phase in PCR?
What is the primary function of the exponential phase in PCR?
