Methods to study gene expression
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What is the purpose of using guanidinium thiocyanate (GTC) in RNA isolation?

  • To visualise RNA molecules on a nitrocellulose membrane
  • To remove rRNA from eukaryotic mRNA
  • To amplify mRNA for PCR
  • To inhibit the activity of RNases (correct)
  • What is the primary function of the Poly A+ selection step in eukaryotic mRNA isolation?

  • To amplify the target RNA molecule
  • To synthesise cDNA from mRNA
  • To remove genomic DNA from the RNA sample
  • To separate mRNA from rRNA (correct)
  • What is the purpose of the northern blotting technique?

  • To synthesize cDNA from mRNA
  • To amplify target RNA molecules
  • To isolate mRNA from eukaryotic cells
  • To visualise and quantify RNA molecules (correct)
  • Which of the following is a characteristic of reverse transcription-PCR?

    <p>Involves the synthesis of cDNA from mRNA</p> Signup and view all the answers

    What is the purpose of the exponential phase in PCR?

    <p>To amplify the target DNA sequence exponentially</p> Signup and view all the answers

    What is the function of SYBR Green in quantitative real-time PCR?

    <p>To preferentially bind to double-stranded DNA</p> Signup and view all the answers

    What is the purpose of using a dual-labelled probe in TaqMan PCR?

    <p>To release a fluorescent dye upon probe cleavage</p> Signup and view all the answers

    What is the purpose of making a cDNA library?

    <p>To clone and express eukaryotic genes in bacteria</p> Signup and view all the answers

    What is the purpose of ligating cDNA to a restriction enzyme-digested vector?

    <p>To create a recombinant vector for bacterial transformation</p> Signup and view all the answers

    What is the purpose of selecting for bacteria with a cDNA insert?

    <p>To identify bacteria that have taken up the recombinant vector</p> Signup and view all the answers

    What is the primary purpose of adding guanidinium thiocyanate (GTC) to the RNA isolation process?

    <p>To inhibit RNAses and prevent RNA degradation</p> Signup and view all the answers

    What is the primary benefit of using Northern analysis (blotting) over other RNA detection methods?

    <p>It allows for the visualization of RNA molecules and their size determination</p> Signup and view all the answers

    What is the primary function of the random hexamers in reverse transcription-PCR?

    <p>To act as a primer for the reverse transcription reaction</p> Signup and view all the answers

    What is the primary advantage of using quantitative real-time PCR over traditional PCR?

    <p>It allows for the real-time measurement of PCR product accumulation</p> Signup and view all the answers

    What is the primary purpose of creating a cDNA library?

    <p>To isolate and clone specific genes of interest</p> Signup and view all the answers

    What is the primary benefit of using oligo dT primers in reverse transcription-PCR?

    <p>They allow for the amplification of RNA molecules with poly A+ tails</p> Signup and view all the answers

    What is the primary purpose of the linear phase in PCR?

    <p>It is the stage where the PCR product accumulates at a constant rate</p> Signup and view all the answers

    What is the primary benefit of using gene-specific primers in reverse transcription-PCR?

    <p>They enhance the specificity of the PCR reaction</p> Signup and view all the answers

    What is the primary purpose of transferring the RNA molecules to a nitrocellulose membrane in Northern analysis?

    <p>To immobilize the RNA molecules for hybridization with a probe</p> Signup and view all the answers

    What is the primary benefit of using reverse transcription-PCR over traditional PCR?

    <p>It allows for the analysis of RNA expression levels</p> Signup and view all the answers

    What is the primary reason why RNA isolation difficulties often arise?

    <p>Presence of RNases in the sample</p> Signup and view all the answers

    What is the primary advantage of using Poly A+ selection in eukaryotic mRNA isolation?

    <p>It helps to remove rRNA from the sample</p> Signup and view all the answers

    What is the primary purpose of transferring the RNA molecules to a nitrocellulose membrane in Northern analysis?

    <p>To immobilize the RNA molecules</p> Signup and view all the answers

    What is the primary function of the quencher in a TaqMan probe?

    <p>To prevent fluorescence when the probe is intact</p> Signup and view all the answers

    What is the primary advantage of using quantitative real-time PCR over traditional PCR?

    <p>It allows for the measurement of PCR products in real-time</p> Signup and view all the answers

    What is the primary purpose of making a cDNA library?

    <p>To study gene expression</p> Signup and view all the answers

    What is the primary function of the random hexamers in reverse transcription-PCR?

    <p>To prime the synthesis of cDNA</p> Signup and view all the answers

    What is the primary advantage of using reverse transcription-PCR over traditional PCR?

    <p>It allows for the analysis of RNA molecules</p> Signup and view all the answers

    What is the primary purpose of ligating cDNA to a restriction enzyme-digested vector?

    <p>To clone the cDNA</p> Signup and view all the answers

    What is the primary function of the exponential phase in PCR?

    <p>To amplify the target DNA sequence</p> Signup and view all the answers

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