RNA and Protein Synthesis Overview
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Questions and Answers

What is the function of ribosomes?

Ribosomes are the organelles that assemble amino acids into proteins.

Which of the following is NOT a function of RNA?

  • Aid in protein synthesis
  • Store genetic information (correct)
  • Carry genetic information from DNA to ribosomes
  • Provide structural support for cells
  • Transcription occurs in the cytoplasm of eukaryotic cells.

    False (B)

    What is the name of the enzyme that carries out transcription?

    <p>RNA polymerase (B)</p> Signup and view all the answers

    Which of the following is NOT a step in gene expression?

    <p>Replication (B)</p> Signup and view all the answers

    What is the function of a promoter in DNA?

    <p>A promoter is a specific DNA sequence that marks the start of a gene and provides a binding site for RNA polymerase.</p> Signup and view all the answers

    What is the name of the process by which introns are removed from a newly transcribed RNA molecule?

    <p>Splicing (A)</p> Signup and view all the answers

    What is the function of a poly-A tail added to a mature mRNA?

    <p>A poly-A tail helps protect the mRNA from degradation, promotes its export from the nucleus, and aids in translation efficiency.</p> Signup and view all the answers

    Which type of RNA carries the genetic code from DNA to ribosomes for protein synthesis?

    <p>mRNA (A)</p> Signup and view all the answers

    A set of three consecutive nucleotides in an mRNA molecule that codes for a specific amino acid is called a ______.

    <p>codon</p> Signup and view all the answers

    Match the following types of RNA with their primary function:

    <p>mRNA = Carries amino acids to ribosomes tRNA = Serves as a template for protein synthesis rRNA = Provides structural support for ribosomes snRNA = Involved in splicing pre-mRNA</p> Signup and view all the answers

    Flashcards

    Ricin

    A highly toxic protein from the castor oil plant, lethal in small doses with no antidote.

    Gene

    A segment of DNA that encodes instructions for building RNA or protein products.

    Transcription

    The process of synthesizing RNA from a DNA template.

    mRNA

    Messenger RNA that carries genetic information from DNA to ribosomes for protein synthesis.

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    Translation

    The process where ribosomes decode the information in mRNA to produce proteins.

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    Ribosome

    A cellular structure where protein synthesis occurs, made up of rRNA and proteins.

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    tRNA

    Transfer RNA that carries amino acids to ribosomes during translation, matching anticodons to codons.

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    Codon

    A sequence of three nucleotides in mRNA that specifies a single amino acid or stop signal during translation.

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    Genetic code

    The set of rules by which information encoded in mRNA is translated into proteins, consisting of 64 codons.

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    Start codon

    The specific codon (AUG) that signals the start of translation, also coding for methionine.

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    Stop codons

    Codons that signal the termination of translation, indicating where the protein synthesis ends.

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    Ribosomal RNA (rRNA)

    RNA that forms the core of the ribosome's structure and catalyzes protein synthesis.

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    Introns

    Non-coding sequences within a gene that are removed during RNA processing.

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    Exons

    Coding sequences that are retained in mRNA after splicing and contribute to the final protein product.

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    Alternative splicing

    The process by which different combinations of exons are joined together to produce different proteins from the same gene.

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    Poly-A tail

    A sequence of adenine nucleotides added to the 3' end of mRNA for stability and export from the nucleus.

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    Promoter

    A DNA sequence where RNA polymerase binds to start transcription of a gene.

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    RNA polymerase

    The enzyme that synthesizes RNA by linking RNA nucleotides together based on a DNA template.

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    Peptide bond

    A bond formed between amino acids during protein synthesis, linking them into a polypeptide chain.

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    Mutations

    Permanent changes in a DNA sequence that can affect gene function or protein production.

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    Base-pair substitution

    A type of mutation where one nucleotide is replaced by another in the DNA sequence.

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    Frameshift mutation

    A mutation caused by a deletion or insertion that shifts the entire reading frame of codons.

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    Sickle-cell anemia

    A genetic disorder caused by a mutation in the beta globin gene, leading to abnormal hemoglobin structure.

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    Hemoglobin

    A protein in red blood cells that carries oxygen, composed of four polypeptides.

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    Eukaryotes

    Organisms whose cells have a nucleus and organelles, including RNA processing in the nucleus.

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    Prokaryotes

    Single-celled organisms without a nucleus, where transcription and translation happen simultaneously.

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    Study Notes

    Ribonucleic Acid (RNA) and Protein Synthesis

    • RNA is a crucial molecule in protein synthesis
    • It's similar to DNA but single-stranded
    • RNA nucleotides have ribose sugar, phosphate groups, and four bases (adenine, uracil, cytosine, guanine)
    • Three main types of RNA involved in protein synthesis: mRNA (messenger), tRNA (transfer), and rRNA (ribosomal)

    Ribosome Inactivating Proteins (RIPs)

    • Ricin is a highly toxic naturally occurring protein
    • Kills by inactivating ribosomes, essential for protein synthesis, in cells
    • Effectively deters insects, birds, mammals etc. from eating castor oil plant seeds
    • Ricin production is a concern as a weapon; no antidote
    • Used in past terrorist attacks and plots
    • A ribosome-inactivating protein (RIP) occurs naturally in some bacteria, mushrooms, algae and other plants
    • Includes food crops like tomatoes, barley, spinach

    Gene Expression

    • Genes carry instructions for building proteins
    • DNA holds genetic information
    • Information in a gene is converted to RNA (transcription)
    • Then converts information in mRNA into a protein (translation)
    • DNA is like an encyclopedia
    • RNA is like a disposable copy of a gene
    • Transcription converts DNA info into RNA, through RNA polymerase
    • Translation converts RNA into a polypeptide
    • Polypeptide folds into a protein
    • Proteins perform various life processes

    Transcription (DNA to RNA)

    • RNA polymerase is the main enzyme
    • Requires a promoter sequence on DNA for binding
    • Non-coding DNA strand acts as a template to make RNA
    • RNA is assembled from RNA nucleotides
    • Base-pairing rules govern assembly

    Translation (RNA to Protein)

    • Ribosomes (comprised of rRNA and proteins) assemble polypeptide chains
    • Takes place in cytoplasm
    • Genetic code (codon-amino acid pairings) dictates order of amino acids
    • tRNAs deliver amino acids to ribosomes
    • Ribosomes connect amino acids with peptide bonds
    • Translation ends when a stop codon is reached

    Mutated Genes and Protein Products

    • Mutations can lead to changes in a gene's product
    • Base-pair substitutions
    • Deletion or insertion of base pairs cause frameshift mutations that alter the entire amino acid sequence
    • Examples: Sickle-cell anemia, beta-thalassemia
    • Mutations can result in a variety of diseases, including various cancers

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    Description

    Explore the essential roles of RNA in protein synthesis, including its structure and types. Learn about ribosome-inactivating proteins like ricin and their implications. This quiz covers the fundamentals of gene expression and the significance of RNA in cellular functions.

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