24 Questions
What is one way that RF testing can provide doctors with information about RA?
By determining the severity of the disease
What are the types of samples that can be used for RF testing?
Either serum or plasma
What is the recommended storage temperature for deep frozen samples?
–25 C or below
What should be avoided when handling frozen samples?
Repeating thawing and freezing
What is the purpose of centrifugation in preparing serum samples?
To remove particles and traces of fibrin
What is the recommended time frame for using deep frozen samples?
Within 3 months
What is the primary purpose of the Rheumatoid Factor (RF) test?
To diagnose rheumatoid arthritis
What is the normal function of rheumatoid factor in the immune system?
To form a complex that the immune system can remove
What is the result of a positive RF test in healthy people?
No significance, as it is a normal occurrence
What is the relationship between RF and autoantibodies?
RF is a type of autoantibody
What is a possible outcome of a positive RF test in addition to RA?
Sjögren's syndrome
What percentage of healthy people can have a positive RF test?
4%
What is the initial step in the qualitative determination on the test plate?
Bring the patient specimens and reagents to room temperature
What is the approximate volume of the patient specimen applied to the test plate?
40 μl
What is the purpose of agitating the test plate with a rotating action?
To mix the patient specimen with the latex particles
What is the indication of distinct agglutination in the qualitative RF test?
The presence of rheumatoid factors at a concentration of 20 I.U./ml
What is the purpose of preparing serial dilutions of the patient specimen in the semi-quantitative test?
To determine the concentration of rheumatoid factors
What is the sensitivity of the semi-quantitative tube test?
Approximately 1 I.U./ml
What is the purpose of coating latex particles with human gamma-globulin (IgG)?
To agglutinate when mixed with samples containing rheumatoid factors
What type of immune complexes are formed in serum and joint fluid by rheumatoid factors?
Both self-association and reaction with native IgG
In which tissues are complex deposition most common?
Tissues with restricted blood flow
What is the significance of a seronegative rheumatoid arthritis?
It has a more favourable prognosis than seropositive
What does an elevated RF titer often indicate?
A severe course and generalized pathological process
What does the term 'seronegative RF' mean?
Rheumatoid factors are detectable neither in the latex agglutination nor in the Waaler-Rose test
Learn about the Rheumatoid Factor (RF) test, which detects a protein in the blood that forms in response to foreign entities like bacteria or viruses. Understand how it relates to rheumatoid arthritis (RA) and autoantibodies.
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