Podcast
Questions and Answers
Where should all resuscitation work be carried out?
Where should all resuscitation work be carried out?
- Empty bijou
- Incubator
- Ethanol-soaked cloth
- Biosafety cabinet (correct)
Which tool should be used to open the culture vial?
Which tool should be used to open the culture vial?
- Sterile loop
- Sterile glass cutter (correct)
- Sterile forceps
- Pasteur pipette
How many agar plates and how much broth are required for the culture?
How many agar plates and how much broth are required for the culture?
- 3 agar plates and 3mL of broth
- 5 agar plates and 5mL of broth (correct)
- 10 agar plates and 10mL of broth
- 2 agar plates and 2mL of broth
What should be done if the glass vial shatters and bits of glass fall inside?
What should be done if the glass vial shatters and bits of glass fall inside?
What should be done with the freeze dried vials upon arrival?
What should be done with the freeze dried vials upon arrival?
What should be done if the powder escapes from the vial?
What should be done if the powder escapes from the vial?
Where can the culture details be found?
Where can the culture details be found?
How long should the culture be left to rehydrate after adding broth?
How long should the culture be left to rehydrate after adding broth?
What should be done with the plastic container and protective cotton inside the glass ampoule?
What should be done with the plastic container and protective cotton inside the glass ampoule?
How many agar plates should one drop of the culture suspension be transferred to?
How many agar plates should one drop of the culture suspension be transferred to?
What should be used to clean the outside of the ampoule?
What should be used to clean the outside of the ampoule?
How long should the plates and bijou be incubated until growth is visible?
How long should the plates and bijou be incubated until growth is visible?
Why should the outside ampoule be cleaned?
Why should the outside ampoule be cleaned?
How long should working cultures (broth) be stored?
How long should working cultures (broth) be stored?
Where can the cleaning protocol for the biosafety cabinet be found?
Where can the cleaning protocol for the biosafety cabinet be found?
What should be done if there is no growth visible after 24 hours?
What should be done if there is no growth visible after 24 hours?
Which of the following steps should be taken when opening a vial?
Which of the following steps should be taken when opening a vial?
What should be done after streaking one bead from the master vial onto an agar plate?
What should be done after streaking one bead from the master vial onto an agar plate?
When should the viability of a new culture be checked?
When should the viability of a new culture be checked?
How many plates should be prepared for each vial when checking culture viability?
How many plates should be prepared for each vial when checking culture viability?
What should be done with the biosafety cabinet before using it?
What should be done with the biosafety cabinet before using it?
What should be done to sterilize gloves before handling the vials?
What should be done to sterilize gloves before handling the vials?
What should be done if there is no growth on the plates after incubation?
What should be done if there is no growth on the plates after incubation?
What should be done if there is growth on the plates after incubation?
What should be done if there is growth on the plates after incubation?
What can be used to generate useful data on inclusivity and exclusivity for each test?
What can be used to generate useful data on inclusivity and exclusivity for each test?
Which color cap should the master vial have?
Which color cap should the master vial have?
What should be written on the working vials?
What should be written on the working vials?
What should be written on the master vial?
What should be written on the master vial?
How many working vials should be labeled for each organism?
How many working vials should be labeled for each organism?
What should be done with the excess liquid from the vials?
What should be done with the excess liquid from the vials?
