30 Questions
Where should all resuscitation work be carried out?
Biosafety cabinet
Which tool should be used to open the culture vial?
Sterile glass cutter
How many agar plates and how much broth are required for the culture?
5 agar plates and 5mL of broth
What should be done if the glass vial shatters and bits of glass fall inside?
Leave it as is and proceed as normal
What should be done with the freeze dried vials upon arrival?
Store them at 2-8°C until use
What should be done if the powder escapes from the vial?
Set the emergency fan on the cabinet, spray with Virkon, let soak and wipe down
Where can the culture details be found?
Culture Collection spreadsheet
How long should the culture be left to rehydrate after adding broth?
5-10 minutes
What should be done with the plastic container and protective cotton inside the glass ampoule?
Discard them
How many agar plates should one drop of the culture suspension be transferred to?
5
What should be used to clean the outside of the ampoule?
Ethanol-soaked cloth or pouring ethanol on it and cleaning with blue roll
How long should the plates and bijou be incubated until growth is visible?
Until growth is visible on plates
Why should the outside ampoule be cleaned?
To ensure that the culture is not contaminated if the glass breaks
How long should working cultures (broth) be stored?
7 days
Where can the cleaning protocol for the biosafety cabinet be found?
UKSOP00012 Cleaning Protocol REV A 2022
What should be done if there is no growth visible after 24 hours?
Leave to incubate for 48 hours
Which of the following steps should be taken when opening a vial?
Set the lid right side up
What should be done after streaking one bead from the master vial onto an agar plate?
Set up working vials from the plates
When should the viability of a new culture be checked?
Whenever a new culture has been resuscitated
How many plates should be prepared for each vial when checking culture viability?
Five plates
What should be done with the biosafety cabinet before using it?
Clean it with Klericide or Virkon
What should be done to sterilize gloves before handling the vials?
Spray them with Klericide
What should be done if there is no growth on the plates after incubation?
Contact Public Health England
What should be done if there is growth on the plates after incubation?
Change the status of the culture to 'beads viable'
What can be used to generate useful data on inclusivity and exclusivity for each test?
MicroSnap range
Which color cap should the master vial have?
Red cap
What should be written on the working vials?
Name of the organism and NCTC/ATCC number
What should be written on the master vial?
Name of the organism, NCTC/ATCC number, and 'M'
How many working vials should be labeled for each organism?
Four
What should be done with the excess liquid from the vials?
Discard into a yellow bin
Test your knowledge on resuscitation techniques and protocols in this quiz. Learn about the importance of carrying out resuscitation work in a biosafety cabinet and the materials required for culturing. See if you can answer questions on agar plates, broth, and incubator settings.
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