Resuscitation Techniques and Culturing
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Questions and Answers

Where should all resuscitation work be carried out?

  • Empty bijou
  • Incubator
  • Ethanol-soaked cloth
  • Biosafety cabinet (correct)
  • Which tool should be used to open the culture vial?

  • Sterile loop
  • Sterile glass cutter (correct)
  • Sterile forceps
  • Pasteur pipette
  • How many agar plates and how much broth are required for the culture?

  • 3 agar plates and 3mL of broth
  • 5 agar plates and 5mL of broth (correct)
  • 10 agar plates and 10mL of broth
  • 2 agar plates and 2mL of broth
  • What should be done if the glass vial shatters and bits of glass fall inside?

    <p>Leave it as is and proceed as normal (B)</p> Signup and view all the answers

    What should be done with the freeze dried vials upon arrival?

    <p>Store them at 2-8°C until use (A)</p> Signup and view all the answers

    What should be done if the powder escapes from the vial?

    <p>Set the emergency fan on the cabinet, spray with Virkon, let soak and wipe down (C)</p> Signup and view all the answers

    Where can the culture details be found?

    <p>Culture Collection spreadsheet (B)</p> Signup and view all the answers

    How long should the culture be left to rehydrate after adding broth?

    <p>5-10 minutes (C)</p> Signup and view all the answers

    What should be done with the plastic container and protective cotton inside the glass ampoule?

    <p>Discard them (A)</p> Signup and view all the answers

    How many agar plates should one drop of the culture suspension be transferred to?

    <p>5 (D)</p> Signup and view all the answers

    What should be used to clean the outside of the ampoule?

    <p>Ethanol-soaked cloth or pouring ethanol on it and cleaning with blue roll (C)</p> Signup and view all the answers

    How long should the plates and bijou be incubated until growth is visible?

    <p>Until growth is visible on plates (D)</p> Signup and view all the answers

    Why should the outside ampoule be cleaned?

    <p>To ensure that the culture is not contaminated if the glass breaks (B)</p> Signup and view all the answers

    How long should working cultures (broth) be stored?

    <p>7 days (B)</p> Signup and view all the answers

    Where can the cleaning protocol for the biosafety cabinet be found?

    <p>UKSOP00012 Cleaning Protocol REV A 2022 (A)</p> Signup and view all the answers

    What should be done if there is no growth visible after 24 hours?

    <p>Leave to incubate for 48 hours (A)</p> Signup and view all the answers

    Which of the following steps should be taken when opening a vial?

    <p>Set the lid right side up (D)</p> Signup and view all the answers

    What should be done after streaking one bead from the master vial onto an agar plate?

    <p>Set up working vials from the plates (C)</p> Signup and view all the answers

    When should the viability of a new culture be checked?

    <p>Whenever a new culture has been resuscitated (A)</p> Signup and view all the answers

    How many plates should be prepared for each vial when checking culture viability?

    <p>Five plates (C)</p> Signup and view all the answers

    What should be done with the biosafety cabinet before using it?

    <p>Clean it with Klericide or Virkon (B)</p> Signup and view all the answers

    What should be done to sterilize gloves before handling the vials?

    <p>Spray them with Klericide (B)</p> Signup and view all the answers

    What should be done if there is no growth on the plates after incubation?

    <p>Contact Public Health England (C)</p> Signup and view all the answers

    What should be done if there is growth on the plates after incubation?

    <p>Change the status of the culture to 'beads viable' (C)</p> Signup and view all the answers

    What can be used to generate useful data on inclusivity and exclusivity for each test?

    <p>MicroSnap range (B)</p> Signup and view all the answers

    Which color cap should the master vial have?

    <p>Red cap (D)</p> Signup and view all the answers

    What should be written on the working vials?

    <p>Name of the organism and NCTC/ATCC number (B)</p> Signup and view all the answers

    What should be written on the master vial?

    <p>Name of the organism, NCTC/ATCC number, and 'M' (C)</p> Signup and view all the answers

    How many working vials should be labeled for each organism?

    <p>Four (B)</p> Signup and view all the answers

    What should be done with the excess liquid from the vials?

    <p>Discard into a yellow bin (C)</p> Signup and view all the answers

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