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Questions and Answers
What is the primary function of restriction endonucleases in recombinant DNA technology?
What is the primary function of restriction endonucleases in recombinant DNA technology?
- To cut DNA at specific sequences (correct)
- To amplify DNA sequences
- To transport DNA into host cells
- To add methyl groups to DNA
What defines the recognition sequence of a restriction enzyme?
What defines the recognition sequence of a restriction enzyme?
- A random location on the DNA strand
- A sequence of four base pairs
- Any nucleotide sequence in the DNA
- A specific sequence of six base pairs (correct)
How are most restriction enzymes named?
How are most restriction enzymes named?
- Based on the genus and species of the bacterial strain from which they were isolated (correct)
- Using the full scientific name of the bacteria
- After the enzyme's mode of action
- By the order of their discovery
What role do vectors play in recombinant DNA technology?
What role do vectors play in recombinant DNA technology?
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Which statement about restriction enzymes is true?
Which statement about restriction enzymes is true?
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Which of the following is NOT a tool of recombinant DNA technology?
Which of the following is NOT a tool of recombinant DNA technology?
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What type of sequences do restriction enzymes typically recognize?
What type of sequences do restriction enzymes typically recognize?
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Study Notes
Restriction Enzymes
- Discovered in 1963, restriction enzymes were the first tools used in genetic engineering
- Restriction enzymes cut DNA at specific sequences called recognition sequences
- Restriction endonuclease Hind II was the first enzyme to be identified
- Hind II recognizes six base pairs in a DNA sequence
- There are over 900 known restriction enzymes, isolated from over 230 bacteria strains
- The naming convention of restriction enzymes names them after their genus and species of origin - for example, EcoRI was isolated from Escherichia coli RY13
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