Podcast
Questions and Answers
What is the role of restriction enzymes in DNA manipulation?
What is the role of restriction enzymes in DNA manipulation?
- Create recombinant vector molecules
- Recognize and cut double-stranded DNA at specific locations (correct)
- Separate DNA fragments using gel electrophoresis
- Attach DNA pieces together
Why do restriction enzymes predictably cut both strands of DNA?
Why do restriction enzymes predictably cut both strands of DNA?
- To separate DNA fragments by size
- Because they recognize palindromic sequences (correct)
- To facilitate gel electrophoresis
- To create new DNA sequences
What is the function of DNA ligase enzymes in DNA manipulation?
What is the function of DNA ligase enzymes in DNA manipulation?
- Recognize recognition sequences for cutting
- Create palindromic sequences
- Separate DNA fragments by size
- Stick cut DNA pieces back together (correct)
Why is it important for the vector and source DNA to be cut with the same restriction enzyme?
Why is it important for the vector and source DNA to be cut with the same restriction enzyme?
What technique is used to separate DNA fragments after they have been cut?
What technique is used to separate DNA fragments after they have been cut?
Why do DNA fragments move during gel electrophoresis?
Why do DNA fragments move during gel electrophoresis?
What do restriction enzymes recognize in order to cut double-stranded DNA?
What do restriction enzymes recognize in order to cut double-stranded DNA?
Why do restriction enzymes predictably cut both strands of DNA?
Why do restriction enzymes predictably cut both strands of DNA?
How do DNA ligase enzymes contribute to DNA manipulation?
How do DNA ligase enzymes contribute to DNA manipulation?
Why is it important for source DNA and vector to be cut with the same restriction enzyme to create a recombinant vector molecule?
Why is it important for source DNA and vector to be cut with the same restriction enzyme to create a recombinant vector molecule?
What is the purpose of using agarose gel in gel electrophoresis?
What is the purpose of using agarose gel in gel electrophoresis?
Which property of DNA fragments allows them to be separated by gel electrophoresis?
Which property of DNA fragments allows them to be separated by gel electrophoresis?
Why are restriction enzymes necessary in creating recombinant vector molecules?
Why are restriction enzymes necessary in creating recombinant vector molecules?
What is the role of DNA ligase enzymes in sticking DNA pieces back together?
What is the role of DNA ligase enzymes in sticking DNA pieces back together?
