14 Questions
What is the role of restriction enzymes in DNA manipulation?
Recognize and cut double-stranded DNA at specific locations
Why do restriction enzymes predictably cut both strands of DNA?
Because they recognize palindromic sequences
What is the function of DNA ligase enzymes in DNA manipulation?
Stick cut DNA pieces back together
Why is it important for the vector and source DNA to be cut with the same restriction enzyme?
To ensure they have the same sticky ends
What technique is used to separate DNA fragments after they have been cut?
Gel electrophoresis
Why do DNA fragments move during gel electrophoresis?
Due to an electric field and agarose gel sieving effect
What do restriction enzymes recognize in order to cut double-stranded DNA?
6 base pair recognition sequences
Why do restriction enzymes predictably cut both strands of DNA?
They recognize unique sequences on both strands
How do DNA ligase enzymes contribute to DNA manipulation?
They stick the cut DNA pieces back together
Why is it important for source DNA and vector to be cut with the same restriction enzyme to create a recombinant vector molecule?
To create complementary sticky ends
What is the purpose of using agarose gel in gel electrophoresis?
To separate DNA fragments by size
Which property of DNA fragments allows them to be separated by gel electrophoresis?
Negative charge
Why are restriction enzymes necessary in creating recombinant vector molecules?
To recognize specific recognition sequences
What is the role of DNA ligase enzymes in sticking DNA pieces back together?
"To seal nicks or breaks in the sugar-phosphate backbone"
Explore how restriction enzymes recognize specific sequences to cut DNA at predictable locations. Learn how DNA ligase enzymes can be used to stick cut DNA pieces back together or to new DNA sequences. Understand the palindromic nature of recognition sequences and their role in cutting both DNA strands.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
