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Restriction Enzymes and DNA Cutting

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Questions and Answers

What is the role of restriction enzymes in DNA manipulation?

Create recombinant vector molecules

Recognize and cut double-stranded DNA at specific locations (correct)

Separate DNA fragments using gel electrophoresis

Attach DNA pieces together

Why do restriction enzymes predictably cut both strands of DNA?

To separate DNA fragments by size

Because they recognize palindromic sequences (correct)

To facilitate gel electrophoresis

To create new DNA sequences

What is the function of DNA ligase enzymes in DNA manipulation?

Recognize recognition sequences for cutting

Create palindromic sequences

Separate DNA fragments by size

Stick cut DNA pieces back together (correct)

Why is it important for the vector and source DNA to be cut with the same restriction enzyme?

To ensure they have the same sticky ends Signup and view all the answers

What technique is used to separate DNA fragments after they have been cut?

Gel electrophoresis Signup and view all the answers

Why do DNA fragments move during gel electrophoresis?

Due to an electric field and agarose gel sieving effect Signup and view all the answers

What do restriction enzymes recognize in order to cut double-stranded DNA?

6 base pair recognition sequences Signup and view all the answers

Why do restriction enzymes predictably cut both strands of DNA?

They recognize unique sequences on both strands Signup and view all the answers

How do DNA ligase enzymes contribute to DNA manipulation?

They stick the cut DNA pieces back together Signup and view all the answers

Why is it important for source DNA and vector to be cut with the same restriction enzyme to create a recombinant vector molecule?

To create complementary sticky ends Signup and view all the answers

What is the purpose of using agarose gel in gel electrophoresis?

To separate DNA fragments by size Signup and view all the answers

Which property of DNA fragments allows them to be separated by gel electrophoresis?

Negative charge Signup and view all the answers

Why are restriction enzymes necessary in creating recombinant vector molecules?

To recognize specific recognition sequences Signup and view all the answers

What is the role of DNA ligase enzymes in sticking DNA pieces back together?

"To seal nicks or breaks in the sugar-phosphate backbone" Signup and view all the answers

Restriction Enzymes and DNA Cutting

Choose a study mode

Podcast

Questions and Answers

What is the role of restriction enzymes in DNA manipulation?

Why do restriction enzymes predictably cut both strands of DNA?

What is the function of DNA ligase enzymes in DNA manipulation?

Why is it important for the vector and source DNA to be cut with the same restriction enzyme?

What technique is used to separate DNA fragments after they have been cut?

Why do DNA fragments move during gel electrophoresis?

What do restriction enzymes recognize in order to cut double-stranded DNA?

Why do restriction enzymes predictably cut both strands of DNA?

How do DNA ligase enzymes contribute to DNA manipulation?

Why is it important for source DNA and vector to be cut with the same restriction enzyme to create a recombinant vector molecule?

What is the purpose of using agarose gel in gel electrophoresis?

Which property of DNA fragments allows them to be separated by gel electrophoresis?

Why are restriction enzymes necessary in creating recombinant vector molecules?

What is the role of DNA ligase enzymes in sticking DNA pieces back together?

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