18 Questions
Which of the following is the primary purpose of using antibiotic resistance to select cells during cloning?
To ensure that only the transformed cells containing the plasmid are able to grow and multiply
In the context of cloning, what is the purpose of blue-white screening?
To visually identify the colonies that contain the cloned gene of interest
Which of the following is the primary function of restriction enzymes in the context of cloning?
To cut the donor DNA into smaller fragments for insertion into the vector
How does bacterial protection from foreign DNA, such as through the use of restriction enzymes, help in the cloning process?
It allows for the selective growth of only the transformed cells
Which of the following techniques is most commonly used to transfer recombinant DNA (rDNA) into host cells during the cloning process?
Electroporation
Which of the following is the primary purpose of using PCR in the context of cloning?
To amplify the cloned DNA fragment to increase the quantity for analysis
Which of the following is the primary purpose of using restriction enzymes in recombinant DNA technology?
To cleave the DNA fragment at specific sequences
What is the primary mechanism by which bacteria protect themselves from the effects of their own recombinant DNA?
Methylation of DNA sequences recognized by restriction enzymes
Which of the following is the primary method used to transfer recombinant DNA into a host cell during the cloning process?
Chemical transformation
Which of the following is the most common selection method used to identify bacterial cells that have successfully incorporated recombinant DNA?
Blue-white screening
Which of the following enzymes is primarily responsible for ligating the DNA fragment into the vector during the construction of recombinant DNA?
DNA ligase
Which of the following is the primary purpose of using the polymerase chain reaction (PCR) in recombinant DNA technology?
To amplify the desired DNA fragment for cloning
Which statement about restriction enzymes and the generation of sticky or cohesive ends is correct?
Sticky ends are produced when restriction enzymes cleave DNA in a staggered manner, leaving short single-stranded overhangs.
How do bacteria protect their own DNA from cleavage by restriction enzymes like EcoRI?
Bacteria modify their DNA by methylating specific nucleotides within the restriction enzyme recognition sequence, preventing cleavage.
Which of the following statements about the transfer of recombinant DNA (rDNA) to cells is correct?
Electroporation and microinjection are two techniques used to introduce rDNA into eukaryotic cells, while transformation is used for prokaryotic cells.
In the context of cloning, what is the purpose of selection during the process of transferring rDNA to cells?
Selection identifies cells that have successfully taken up the rDNA construct, allowing for their isolation and further propagation.
Which of the following statements about the use of enzymes in biotechnology is correct?
All of the above statements about the use of enzymes in biotechnology are correct.
Which of the following statements regarding bacterial transformation is correct?
A bacterial cell that is capable of undergoing transformation is referred to as 'competent', meaning it can take up exogenous DNA.
Explore how restriction enzymes function in cutting DNA to create fragments with different types of ends. Learn about cohesive and blunt ends and how bacteria protect their DNA from these enzymes.
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