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Restriction Endonucleases Quiz

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Questions and Answers

What is the main function of restriction enzymes in bacteria?

Aiding in DNA replication

Regulating gene expression

Defending against viral infection (correct)

Assisting in protein synthesis

How many commonly used restriction enzymes have been identified?

150 (correct)

3500

500

What determines the size of restriction fragments produced by restriction enzymes?

The number of times a given restriction enzyme cuts the DNA (correct)

The temperature of the reaction

The concentration of the DNA sample

The type of bacteria producing the enzyme

How often will the enzyme AluI cut DNA if it recognizes the sequence AGCT?

Every 256 base pairs (A) Signup and view all the answers

What is the specific nucleotide sequence recognized by a restriction enzyme called?

Recognition sequence (C) Signup and view all the answers

What is the function of restriction enzymes in bacteria?

To defend against viral infection by degrading viral DNA (A) Signup and view all the answers

How many restriction enzymes are commonly used by researchers?

About 150 (B) Signup and view all the answers

What does a restriction enzyme recognize on DNA?

A specific nucleotide sequence (B) Signup and view all the answers

How does the size of restriction fragments vary?

Based on the number of times a given restriction enzyme cuts the DNA (B) Signup and view all the answers

What determines the average distance between cuts for a restriction enzyme with a four-base recognition sequence?

The number of base pairs in the recognition sequence (A) Signup and view all the answers