Respirometer Practical: Temperature Effects

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Questions and Answers

What does the following reaction represent? $C_6H_{12}O_6 \rightarrow C_2H_5OH + CO_2 + 2ATP$

  • Aerobic respiration in animals
  • Lactic acid fermentation in muscles
  • Photosynthesis in plants
  • Anaerobic respiration in yeast (correct)

What is being described by the statement: 'The volume of oxygen needed to break down lactic acid produced by anaerobic respiration'?

  • The oxygen debt (correct)
  • The tidal volume
  • The rate of oxygen consumption
  • The anaerobic threshold

Why does the temperature of flasks containing live seeds and dead seeds increase during a respiration experiment?

  • The flask absorbs heat from surrounding
  • The dead seeds release heat due to decay
  • Respiration produces heat, releasing energy as ATP (correct)
  • The seeds decompose to produce heat

Which factor is LEAST likely to affect the rate of respiration in a practical setup?

<p>Type of seed (C)</p> Signup and view all the answers

What experimental procedural precaution is mentioned as being used in order to produce reliable data?

<p>Using the same type of seed (B)</p> Signup and view all the answers

Flashcards

Anaerobic Respiration in Yeast

The process where glucose is broken down into ethanol and carbon dioxide, releasing a small amount of energy (ATP).

Oxygen Debt

The amount of oxygen needed to break down lactic acid produced during anaerobic respiration.

Muscle Fatigue

The inability of muscles to contract due to a lack of oxygen. This happens when the body cannot supply enough oxygen during intense physical activity.

Respiration and Heat Production

Respiration releases energy in the form of heat, causing the temperature of the living system to rise.

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Measuring Respiration Rate

The rate of respiration can be measured by the rate at which carbon dioxide is produced or oxygen is consumed.

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Study Notes

Respirometer Practical

  • A respirometer measures the rate of respiration in organisms by measuring the consumption of oxygen.
  • The apparatus shows how the rate changes in relation to factors.
  • The rate of respiration is calculated by dividing the distance travelled by the liquid in the manometer by the time.
  • Controls include similar apparatus with glass beads, dead maggots, or appropriate controls.

Effect of Temperature on Respiration

  • Temperature is an independent variable in the effect of temperature on respiration, while the rate is the dependent variable.
  • Experiment variables include similar seeds, mass of the organism, and the volume of sodium hydroxide.
  • Results will show the distance the liquid in the manometer travels to calculate the rate of respiration.
  • The results should be presented with comparison measurements for the live and dead seeds to show increases over time in both but the temperatures should differ.
  • A control experiment (dead seeds) is used to compare results with live seeds.

Anaerobic Respiration

  • Anaerobic respiration is a process which does not require oxygen. It is the process of creating ATP without oxygen.
  • The reaction in yeast is glucose to ATP, carbon dioxide, and ethanol. Equation: C₆H₁₂O₆ --> C₂H₅OH + CO₂ + 2ATP
  • Oxygen debt is the amount of oxygen needed to break down lactic acid.

Calculating Respiration Rate

  • The rate of respiration is often calculated using figures measured in the respirometer experiment, this could involve the rate in mm/min, and calculations about the volume of oxygen consumed. The formula for the volume of a cylinder is V = π * r² * h, where 'h' is the distance the liquid moves.

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