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Questions and Answers
What type of molecules can reducing sugars give away electrons to?
What type of molecules can reducing sugars give away electrons to?
Which of the following is NOT a type of reducing sugar?
Which of the following is NOT a type of reducing sugar?
What is the purpose of heating the test tube in a boiling water bath during the Benedict's test?
What is the purpose of heating the test tube in a boiling water bath during the Benedict's test?
What is the color of the Benedict's reagent before the reaction?
What is the color of the Benedict's reagent before the reaction?
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What is the purpose of using a calibration card with reagent test strips?
What is the purpose of using a calibration card with reagent test strips?
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What is the primary purpose of using test strips in medical situations?
What is the primary purpose of using test strips in medical situations?
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What is the characteristic of non-reducing sugars?
What is the characteristic of non-reducing sugars?
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What is the purpose of adding hydrochloric acid to a sample when testing for non-reducing sugars?
What is the purpose of adding hydrochloric acid to a sample when testing for non-reducing sugars?
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What is the result of the iodine test when starch is present in a sample?
What is the result of the iodine test when starch is present in a sample?
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What is the purpose of adding sodium hydrogen carbonate to a sample when testing for non-reducing sugars?
What is the purpose of adding sodium hydrogen carbonate to a sample when testing for non-reducing sugars?
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Study Notes
Reducing Sugars
- Reducing sugars are a type of carbohydrate that can give away electrons to reduce other molecules.
- Examples of reducing sugars include monosaccharides (glucose, galactose, fructose) and most disaccharides (maltose, lactose).
- The Benedict's test is a procedure used to detect the presence of reducing sugars.
- The test involves adding Benedict's reagent (copper(II) sulfate) to a sample, heating it in a boiling water bath, and observing the color change.
- If reducing sugars are present, the copper(II) ions are reduced to copper(I) ions, forming an orangey-red precipitate (copper(I) oxide).
- The color change indicates the presence of reducing sugars, and the strength of the color change indicates the concentration of the sugar.
Benedict's Test
- The test tube is heated in a boiling water bath for 3-5 minutes to allow the reaction to occur.
- The Benedict's reagent is blue before the reaction, and changes color to green, yellow, or orange-red if reducing sugars are present.
- If the reaction mixture stays blue, there are no reducing sugars present.
- The color change can be used to estimate the initial concentration of reducing sugars.
Reagent Test Strips
- Reagent test strips are used to test for reducing sugars.
- The strip is dipped into the sample, and the resulting color is matched to a calibration card to estimate the concentration of reducing sugars.
- The test strips are commonly used in medical situations to test urine samples for diabetic patients.
Non-Reducing Sugars
- Non-reducing sugars are carbohydrates that do not give away electrons to reduce other molecules.
- Examples of non-reducing sugars include some disaccharides (sucrose).
- The Benedict's test does not work with non-reducing sugars.
- To test for non-reducing sugars, hydrochloric acid is added to the sample to break it down into its monosaccharides, which are then reducing sugars.
- The sample is then neutralized with sodium hydrogen carbonate, and the Benedict's test is repeated.
Starch
- Starch is a carbohydrate that can be tested for using the iodine test.
- The iodine test involves adding potassium iodide solution to the sample and gently shaking the tube.
- If starch is present, the solution changes color from yellow-brown to blue-black.
Reducing Sugars
- Reducing sugars are a type of carbohydrate that can give away electrons to reduce other molecules.
- Examples of reducing sugars include monosaccharides (glucose, galactose, fructose) and most disaccharides (maltose, lactose).
- The Benedict's test is a procedure used to detect the presence of reducing sugars.
Benedict's Test
- The test involves adding Benedict's reagent (copper(II) sulfate) to a sample, heating it in a boiling water bath, and observing the color change.
- Copper(II) ions are reduced to copper(I) ions, forming an orangey-red precipitate (copper(I) oxide) if reducing sugars are present.
- The color change indicates the presence of reducing sugars, and the strength of the color change indicates the concentration of the sugar.
- The test tube is heated in a boiling water bath for 3-5 minutes to allow the reaction to occur.
- The Benedict's reagent is blue before the reaction, and changes color to green, yellow, or orange-red if reducing sugars are present.
Reagent Test Strips
- Reagent test strips are used to test for reducing sugars.
- The strip is dipped into the sample, and the resulting color is matched to a calibration card to estimate the concentration of reducing sugars.
- The test strips are commonly used in medical situations to test urine samples for diabetic patients.
Non-Reducing Sugars
- Non-reducing sugars are carbohydrates that do not give away electrons to reduce other molecules.
- Examples of non-reducing sugars include some disaccharides (sucrose).
- The Benedict's test does not work with non-reducing sugars.
- To test for non-reducing sugars, hydrochloric acid is added to the sample to break it down into its monosaccharides, which are then reducing sugars.
Starch
- Starch is a carbohydrate that can be tested for using the iodine test.
- The iodine test involves adding potassium iodide solution to the sample and gently shaking the tube.
- If starch is present, the solution changes color from yellow-brown to blue-black.
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Description
Identify the characteristics and detection methods of reducing sugars, including monosaccharides and disaccharides, and understand the Benedict's test procedure.