Recombinant Protein Expression Systems Quiz

Which factor is important in determining the type of expression system used to produce recombinant proteins?

What does the type of post-translational modifications impact in the expression system for recombinant proteins?

Why is the destination of the expressed protein an important factor in selecting an expression system?

Which factor is important in determining the type of expression system used to produce recombinant proteins?

Why is the mass of the protein an important factor in selecting an expression system?

What role does the number of disulfide bonds play in the type of expression system selected for recombinant proteins?

Factors Influencing Expression System Selection

Type of Host Organism: Bacterial, yeast, insect, or mammalian systems each have unique advantages for producing different recombinant proteins based on their biology and capabilities.
Post-Translational Modifications: The type and extent of modifications like glycosylation, phosphorylation, and lipidation can affect protein functionality, stability, and immune recognition, necessitating an appropriate expression system capable of performing these modifications.
Destination of the Protein: The localization (intracellular, extracellular, membrane-bound) affects the choice of expression system since some systems may excel at secreting proteins, while others are better for intracellular function.
Mass of the Protein: Larger proteins may require expression systems that can accommodate complex folding pathways and higher yields, influencing the choice based on the system's capacity to produce and properly fold heavy proteins.
Number of Disulfide Bonds: Proteins with multiple disulfide bonds typically need to be expressed in systems like mammalian cells or in specialized bacterial strains designed to facilitate correct folding and bond formation, as improper bonding can lead to non-functional proteins.

Take the quiz to test your knowledge on factors that determine the type of expression system used to produce recombinant proteins. Assess your understanding of time, handling, protein quantity, mass, post-translational modifications, disulfide bonds, and protein destination in the expression process.